| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The mechanisms of muscle protein synthesis and degradation by citrulline which is not used for protein synthesis were elucidated using C2C12 myotube cells. C2C12 cells treated with citrulline showed suppression of protein degradation through autophagy/lysosome proteolytic system. It is suggested that this suppression was regulated by mTOR. Intracellular arginine concentration was not increased by citrulline treatment, therefore the effects of citrulline may due to citrulline itself.
Saccharopine, a metabolite of lysine, suppressed protein degradation in C2C12 myotube cells by reduction of autophagy/lysosome proteolytic system. This suppression is considered to be regulated by Akt/mTOR.
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