Establishment of a culturing system that enables selective induction of specific cardiac cell types from human pluripotent stem cells
Project/Area Number |
24615003
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Regenerative medicine
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Research Institution | Kyoto University |
Principal Investigator |
YAMAUCHI Kaori 京都大学, 再生医科学研究所, 研究員 (50503653)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 心筋細胞 / 多能性幹細胞 / 分化 / 分化誘導 / ヒト胚性幹細胞 |
Outline of Final Research Achievements |
Human pluripotent stem cell (hPSC)-derived cardiomyocytes are expected to be a valuable cell source for transplantation therapies. Although many methods to obtain a large amount of the cardiomyocytes from the hPSCs have been developed, effective protocols for selective induction of the specific cardiac cell types such as ventricular, atrial, and pacemaker cells are yet to be established. To address the issue, we investigated the expression of cell surface proteins at different stages of cardiac development and found some markers that may be useful to identify the developmental origin of cardiomyocytes. We also established NKX2-5eGFP / MLC2vmCherry hESCs, which enable labeling, tracing and purifying the ventricular-specific cells from the differentiated cells. The culture system we established in the study allows monitoring the development process of the cardiac tissue cells from early to late stages.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells2014
Author(s)
Ken Igawaa, Chikara Kokubu, Kosuke Yusa, Kyoji Horie, Yasuhide Yoshimura, Kaori Yamauchi, Hirofumi Suemori, Hiroo Yokozeki, Masashi Toyoda, Nobutaka Kiyokawa, Hajime Okita, Yoshitaka Miyagawa, Hidenori Akutsu, Akihiro Umezawa, Ichiro Katayama, Junji Takeda.
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Journal Title
Stem Cells Transl Med
Volume: 3
Issue: 9
Pages: 992-1001
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A chemical probe that labels human pluripotent stem cells2014
Author(s)
Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato S, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M
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Journal Title
Cell reports
Volume: 6
Issue: 6
Pages: 1165-74
DOI
NAID
Related Report
Peer Reviewed / Open Access
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