| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Human pluripotent stem cell (hPSC)-derived cardiomyocytes are expected to be a valuable cell source for transplantation therapies. Although many methods to obtain a large amount of the cardiomyocytes from the hPSCs have been developed, effective protocols for selective induction of the specific cardiac cell types such as ventricular, atrial, and pacemaker cells are yet to be established. To address the issue, we investigated the expression of cell surface proteins at different stages of cardiac development and found some markers that may be useful to identify the developmental origin of cardiomyocytes. We also established NKX2-5eGFP / MLC2vmCherry hESCs, which enable labeling, tracing and purifying the ventricular-specific cells from the differentiated cells. The culture system we established in the study allows monitoring the development process of the cardiac tissue cells from early to late stages.
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