Characterization of neuronal regenerating factors secreted from rat bone marrow stromal cells
| Project/Area Number |
24615007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Aino University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IDE Chizuka 藍野大学, 医療保健学部・再生医療研究所, 教授 (70010080)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 神経再生 / 骨髄間質細胞 / 脊髄損傷 |
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| Outline of Final Research Achievements |
There are no effective therapies for spinal cord injury (SCI). We have reported that transplantation of bone marrow stromal cells (BMSCs) has beneficial effects on SCI. In the present study, we analyzed trophic factors secreted from BMSCs. At first, we improved the neural bioassay of neurite outgrowth using cultured hippocampal neurons of newborn rats. Next, we collected serum-free conditioned medium (CM) of rat BMSCs. We purified the effective factors on SCI from the CM by combination of liquid chromatographies and identified them using mass spectrometry systems.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Effects of bone marrow stromal cell transplantation through CSF on the subacute and chronic spinal cord injury in rats2013
Author(s)
Nakano N, Nakai Y, Seo TB, Homma T, Yamada Y, Ohta M, Suzuki Y, Nakatani T, Fukushima M, Hayashibe M, Ide C
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Journal Title
PloS ONE
Volume: 8(9)
Issue: 9
Pages: e73494-e73494
DOI
Related Report
Peer Reviewed
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[Presentation] Inhibitory effect of ETAS against amyloid beta-induced cellular disorder in PC12 cells.2013
Author(s)
Ogasawara J., Ito T., Takanari J., Sakurai T., Kizaki T., Sato S., Ishibashi Y., Nagasawa J., Saito D., Nakano N., Izawa T., and Ohno H.
Organizer
The 21st International Congress on Nutrition and Integrative Medicine
Place of Presentation
Sapporo
Related Report
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