| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We developed Live single-cell MS method which allows serial analysis from single cell content recovery to direct ionization of included molecules extracted with ionization solvent using a nanospray chip followed by mass spectrometric measurement. In order to apply this method for low-polar molecules, we made a nanospray-oscillation device using a piezo element to nebulize solvent. Ionization was achieved by a corona discharge device. We found that signal of low-polar molecules was increased whereas background signal of environmental origin was also increased. To improve background noise, we made a device for ionization in clean charcoal-treated nitrogen gas-environment. We confirmed that signals of low-polar molecules were increased compared to the original method.
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