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Fundamental study for development of biomonitoring system using accurate corona discharges and mass spectrometry

Research Project

Project/Area Number 24619005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field オミクス計測科学
Research InstitutionYokohama City University

Principal Investigator

SEKIMOTO Kanako  横浜市立大学, 生命ナノシステム科学研究科, 助教 (40583399)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsコロナ放電 / 質量分析 / 大気イオン / 負イオン / プロトン親和力 / アミノ酸 / ペチュニア / リアルタイム直接分析法(DART) / 反応イオン / 大気反応イオン
Outline of Final Research Achievements

One of the most interesting topics in omics is the development of in situ analysis techniques for biomolecules. In this study, a biomonitoring system that makes it possible to ionize low molecular weight biomolecules selectively and in situ has been developed using accurate corona discharges and mass spectrometry. The accurate corona discharges lead to the regular formation of atmospheric negative ions with various different gas-phase acidities, which serve as reactant ions to ionize gas-phase analytes via ion-molecule reactions. The system developed here can provide the information about both molecular weight and structure of a given analyte, which contributes towards the application to unknown compound analysis.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (30 results)

All 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 10 results,  Acknowledgement Compliant: 2 results) Presentation (14 results) (of which Invited: 3 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Ionization characteristics of amino acids in direct analysis in real time mass spectrometry2014

    • Author(s)
      K. Sekimoto, M. Sakakura, T. Kawamukai, H. Hike, T. Shiota, F. Usui, Y. Bando and M. Takayama
    • Journal Title

      Analyst

      Volume: 139 Issue: 10 Pages: 2589-2599

    • DOI

      10.1039/c3an02193a

    • NAID

      120006530537

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Formation of Benzyl Carbanion in Collision-Induced Dissociation of Deprotonated Phenylalanine Homologues2014

    • Author(s)
      K. Sekimoto, N. Matsuda and M. Takayama
    • Journal Title

      Mass Spectrometry

      Volume: 3 Issue: 1 Pages: A0027-A0027

    • DOI

      10.5702/massspectrometry.A0027

    • NAID

      130004972829

    • ISSN
      2186-5116, 2187-137X
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Field measurement of nitromethane from automotive emissions at a busy interaction using proton-transfer-reaction mass spectrometry2014

    • Author(s)
      S. Inomata, Y. Fujitani, A. Fushimi, H. Tanimoto, K. Sekimoto and H. Yamada
    • Journal Title

      Atmospheric Environment

      Volume: 96 Pages: 301-309

    • DOI

      10.1016/j.atmosenv.2014.07.058

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] <b>Application of Mass Spectrometry to Studies on Secondary Organic Aerosol Formation </b>2014

    • Author(s)
      廣川淳、猪俣敏、高橋けんし、坂本陽介、谷本浩志、関本奏子
    • Journal Title

      Earozoru Kenkyu

      Volume: 29 Issue: S1 Pages: s18-s26

    • DOI

      10.11203/jar.29.s18

    • NAID

      130003393425

    • ISSN
      0912-2834, 1881-543X
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] コロナが拓く新技術2014

    • Author(s)
      関本 奏子
    • Journal Title

      ケミカルエンジニヤリング

      Volume: 59 Pages: 46-53

    • Related Report
      2013 Research-status Report
  • [Journal Article] Collision-induced dissociation analysis of negative atmospheric ion adducts in atmospheric pressure corona discharge ionization mass spectrometry2013

    • Author(s)
      K. Sekimoto and M. Takayama
    • Journal Title

      J. Am. Soc. Mass Spectrom.

      Volume: 24 Issue: 5 Pages: 780-788

    • DOI

      10.1007/s13361-013-0576-2

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] On-line measurements of gaseous nitro-organic compounds in diesel vehicle exhaust by proton-transfer-reaction mass spectrometry2013

    • Author(s)
      S. Inomata, H. Tanimoto, Y. Fujitani, K. Sekimoto, K. Sato, A. Fushimi, H. Yamada, S. Hori, Y. Kumazawa, A. Shimono and T. Hikida
    • Journal Title

      Atmospheric Environment

      Volume: 73 Pages: 195-203

    • DOI

      10.1016/j.atmosenv.2013.03.035

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Collision-Induced Dissociation Study of the Adduct Ions Produced in NO<sub>3</sub><sup>−</sup>-Free Area of Atmospheric Pressure Negative Corona Discharges under Ambient Air Conditions2013

    • Author(s)
      K. Sekimoto, N. Matsuda, M. Takayama
    • Journal Title

      Mass Spectrometry

      Volume: 2 Issue: 1 Pages: A0020-A0020

    • DOI

      10.5702/massspectrometry.A0020

    • NAID

      130003380161

    • ISSN
      2186-5116, 2187-137X
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of nitromethane emission from automotive exhaust”, Atmospheric Environment2013

    • Author(s)
      K. Sekimoto, S. Inomata, H. Tanimoto, A. Fushimi, Y. Fujitani, K. Sato and H. Yamada
    • Journal Title

      Atmospheric Environment

      Volume: 81 Pages: 523-531

    • DOI

      10.1016/j.atmosenv.2013.09.031

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mass Spectrometric Study of Negative Atmospheric Ion Water Clusters Formed by Atmospheric Pressure Corona Discharges2013

    • Author(s)
      関本 奏子,高山 光男
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 62 Issue: 11 Pages: 955-963

    • DOI

      10.2116/bunsekikagaku.62.955

    • NAID

      10031196843

    • ISSN
      0525-1931
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Surface analysis of traces stained with discharge on plane electrode in atmospheric pressure negative corona2012

    • Author(s)
      Kanako Sekimoto and Mitsuo Takayama
    • Journal Title

      Journal of Electrostatics

      Volume: 70 Issue: 4 Pages: 363-373

    • DOI

      10.1016/j.elstat.2012.05.004

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Ionization characteristics of amino acids in direct analysis in real time mass spectrometry using atmospheric pressure helium plasma2015

    • Author(s)
      K. Sekimoto and M. Takayama
    • Organizer
      20th Symposium on Application of Plasma Processes
    • Place of Presentation
      Tatranska Lomunica, Slovakia
    • Year and Date
      2015-01-17 – 2015-01-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Formation of benzyl carbanion in collision-induced dissociation of deprotonated phenylalanine homologues2014

    • Author(s)
      K. Sekimoto, N. Matsuda and M. Takayama
    • Organizer
      20th International Mass Spectrometry Conference
    • Place of Presentation
      Geneva, Switzerland
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Ionization mechanism of amino acids in direct analysis in real time-mass spectrometry (DART-MS)2014

    • Author(s)
      K. Sekimoto, M. Sakakura, T. Kawamukai, H. Hike, T. Shiota, F. Usui, Y. Bando and M. Takayama
    • Organizer
      The 5th Asia Oceania Mass Spectrometry Conference
    • Place of Presentation
      Beijing, China
    • Year and Date
      2014-07-16 – 2014-07-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Ionization characteristics of amino acids in direct analysis in real time-mass spectrometry (DART-MS)2014

    • Author(s)
      K. Sekimoto, M. Sakakura, T. Kawamukai, H. Hike, T. Shiota, F. Usui, Y. Bando and M. Takayama
    • Organizer
      The 62th ASMS Conference on Mass Spectrometry
    • Place of Presentation
      Baltimore, MD, USA
    • Year and Date
      2014-06-15 – 2014-06-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Mass spectrometric analysis of negative ion formation in atmospheric pressure corona discharges with point-to-plane electrodes2014

    • Author(s)
      K. Sekimoto and M. Takayama
    • Organizer
      5th International Conference on Plasma Medicine
    • Place of Presentation
      Nara, Japan
    • Year and Date
      2014-05-18 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] リアルタイム直接分析(DART)法におけるアミノ酸のイオン化メカニズム2014

    • Author(s)
      関本 奏子,坂倉 幹始,川向 孝知,比毛 浩,塩田 晃久,碓井 史彦,板東 泰彦,高山 光男
    • Organizer
      第62回質量分析総合討論会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-14 – 2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Molecular mass determination using collision-induced dissociation of negative atmospheric ion adducts generated in ambient corona discharges2013

    • Author(s)
      Kanako Sekimoto, Mitsuo Takayama
    • Organizer
      The 61th ASMS Conference on Mass Spectrometry
    • Place of Presentation
      Minneapolis, MN, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Collision-induced dissociation study of negative atmospheric ion adducts in atmospheric pressure corona discharge ionization mass spectrometry2013

    • Author(s)
      Kanako Sekimoto, Mitsuo Takayama
    • Organizer
      The 4th Asia Oceanic Mass Spectrometry Conference
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Effects of ionic and neutral species on Esherichia coli (E. coli) inactivation in atmospheric pressure corona discharges2013

    • Author(s)
      Kanako Sekimoto, Rena Gonda, Mitsuo Takayama
    • Organizer
      The 21th International Symposium on Plasma Chemistry
    • Place of Presentation
      Cairns, Australia
    • Related Report
      2013 Research-status Report
  • [Presentation] 負極性コロナ放電内のNO3- フリー領域におけるイオン化メカニズム2013

    • Author(s)
      関本 奏子,高山 光男
    • Organizer
      第61回質量分析総合討論会
    • Place of Presentation
      つくば,日本
    • Related Report
      2013 Research-status Report
  • [Presentation] Ionization of organic compounds using negative atmospheric ions formed in atmospheric pressure corona discharges2013

    • Author(s)
      Kanako Sekimoto, Mitsuo Takayama
    • Organizer
      19th Symposium on Application of Plasma Processes
    • Place of Presentation
      Vratna, Slovakia
    • Related Report
      2012 Research-status Report
  • [Presentation] pplication of negative atmospheric ions formed in corona discharges to specific analyte ionization under ambient conditions2012

    • Author(s)
      Kanako Sekimoto, Mitsuo Takayama
    • Organizer
      The 60th ASMS Conference on Mass Spectrometry
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Hydrogen cyanide formation in atmospheric pressure DC corona discharges2012

    • Author(s)
      Kanako Sekimoto, Mitsuo Takayama
    • Organizer
      The 21th Europhysics Conference on the Atomic and Molecular Physics of Ionized Gases
    • Place of Presentation
      Viana do Castelo, Portugal
    • Related Report
      2012 Research-status Report
  • [Presentation] Specific analyte ionization using negative atmospheric ions formed in atmospheric pressure corona discharges2012

    • Author(s)
      Kanako Sekimoto, Mitsuo Takayama
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Research-status Report
  • [Book] 現代質量分析学(第5章 イオン化法3;アンビエントイオン化法)2013

    • Author(s)
      関本 奏子,高山 光男
    • Total Pages
      400
    • Publisher
      化学同人
    • Related Report
      2013 Research-status Report
  • [Book] 現代質量分析学(第5章 イオン化法3;アンビエントイオン化法)2013

    • Author(s)
      高山光男,早川滋雄,瀧浪欣彦,和田芳直 編(関本奏子.高山光男)
    • Total Pages
      500
    • Publisher
      化学同人
    • Related Report
      2012 Research-status Report
  • [Remarks] アンビエントイオン化質量分析における世界最小のイオン源が販売開始

    • URL

      http://www.yokohama-cu.ac.jp/univ/pr/press/140610.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 横浜市立大学 質量分析学研究室

    • URL

      http://masspecs.sci.yokohama-cu.ac.jp

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] イオン化装置及び質量分析装置2013

    • Inventor(s)
      関本 奏子,高山 光男,奥村 大輔
    • Industrial Property Rights Holder
      株式会社島津製作所・公立大学法人横浜市立大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-08-02
    • Related Report
      2013 Research-status Report
    • Overseas

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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