Development of Novel Analytical Method for Amine-containing Metabolites and Application to Metabolomics-like Technique
| Project/Area Number |
24619009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TODOROKI KENICHIRO 静岡県立大学, 薬学部, 准教授 (70341451)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA HIDEYUKI 福岡大学, 薬学部, 准教授 (20301690)
HAYAMA TADASHI 福岡大学, 薬学部, 准教授 (90549693)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | フルオラスケミストリー / アミノ酸 / タンデムマス法 / メタボロミクス / 分析化学 / 質量分析 / 固相抽出 / フルオラス分離技術 / アミン性代謝物 |
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| Outline of Final Research Achievements |
In this study, we developed a novel direct tandem mass spectrometric method for amine-containing metabolites including amino acids utilizing a fluorous derivatization and purification technique. Amino acids were perfluoroalkylated with 2H,2H,3H,3H-perfluoroundecan-1-al in the presence of 2-picoline borane via reductive amination. The derivatives were purified by perfluoroalkyl-modified silica-based monolithic solid-phase extraction (monolithic F-SPE), and directly analyzed by tandem mass spectrometry using electrospray ionization without liquid chromatographic separation. The perfluoroalkyl derivatives could be sufficiently distinguished from non-fluorous compounds, i.e. the biological matrix, due to their fluorous interaction. Thus, rapid and accurate determination of amino acids was accomplished. The method was validated with human plasma samples and applied to the analysis of amino acids in the plasma of mice with maple syrup urine disease or phenylketonuria.
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Report
(5 results)
Research Products
(60 results)
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[Journal Article] Assessment of the Efficacy of Anticancer Drugs by Amino Acid Metabolomics Using Fluorescence Derivatization-HPLC2014
Author(s)
Ryoko Tomita, Kenichiro Todoroki, Kazuyuki Machida, Sho Nishida, Hiroshi Maruoka, Hideyuki Yoshida, Toshihiro Fujioka, Manabu Nakashima, Masatoshi Yamaguchi, Hitoshi Nohta
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Journal Title
Analytical Sciences
Volume: 30
Issue: 7
Pages: 751-758
DOI
NAID
ISSN
0910-6340, 1348-2246
Related Report
Peer Reviewed / Open Access
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[Journal Article] Correlation Between the Effects of Local and Intracerebroventricular Infusions of Galanin on 5-HT Release Studied by Microdialysis, and Distribution of Galanin and Galanin Receptors in Prefrontal Cortex, Ventral Hippocampus, Amygdala, Hypothalamus, and Striatum of Awake Rats2014
Author(s)
Shimako Yoshitake, Eugenia Kuteeva, Tomas Hokfelt, Francoise Mennichen, Elvar Theodorsson, Masatoshi Yamaguchi, Jan Kehr, Takashi Yoshitake
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Journal Title
Synapse
Volume: 68
Issue: 5
Pages: 179-193
DOI
Related Report
Peer Reviewed
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