| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
We designed two types of the chemical probes to induce the ionization of the target molecules. Matrix-like compound of 2-anthoracencarboxylic acid (2-AC) was directly bound to the target molecule of substance P (Sub-P). The complex compound (Sub-P/2-AC) was able to be ionized in MALDI-MS. In addition, 1-AC and 9-AC did not work. We could not identify that the complexes of Sub-P/1-AC and Sub-P/9-AC were synthesized or the complexes could be ionized in MALDI-MS.
In another way, the target molecules are selected with amino-cyclodextrin by the host-guest interaction, and the selected molecules can be ionized selectly because the amino group can be a charge center in amino-cyclodextrin. Sesamin is a labile compound and decomposes in (+)ESI-MS, but the complex of sesamin and amino-cyclodextrin can be detected in ESI-MS without the decomposition. In this case, the target molecules interacting with cyclodextrin can be ionized as the host-guest complex.
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