Ionization chemical probes for selected target molecules with click reaction
| Project/Area Number |
24619010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Suntory Foundation for Life Sciences |
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Principal Investigator |
YAMAGAKI Tohru 公益財団法人サントリー生命科学財団, その他部局等, 研究員 (40313209)
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| Co-Investigator(Kenkyū-buntansha) |
HORIKAWA Manabu 公益財団法人サントリー生命科学財団
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 質量分析 / イオン化 / ホスト・ゲスト / 相互作用 / ionization / cyclodextrin / host-guest / ESI-MS / イメージングMS / 植物花弁 / フラボノイド / 配糖化 / 糖鎖 / 化学プローブ / イメージング / 生命動態 |
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| Outline of Final Research Achievements |
We designed two types of the chemical probes to induce the ionization of the target molecules. Matrix-like compound of 2-anthoracencarboxylic acid (2-AC) was directly bound to the target molecule of substance P (Sub-P). The complex compound (Sub-P/2-AC) was able to be ionized in MALDI-MS. In addition, 1-AC and 9-AC did not work. We could not identify that the complexes of Sub-P/1-AC and Sub-P/9-AC were synthesized or the complexes could be ionized in MALDI-MS.
In another way, the target molecules are selected with amino-cyclodextrin by the host-guest interaction, and the selected molecules can be ionized selectly because the amino group can be a charge center in amino-cyclodextrin. Sesamin is a labile compound and decomposes in (+)ESI-MS, but the complex of sesamin and amino-cyclodextrin can be detected in ESI-MS without the decomposition. In this case, the target molecules interacting with cyclodextrin can be ionized as the host-guest complex.
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Report
(4 results)
Research Products
(8 results)
