Ion characterization using energy-resolved ion-mobility tandem mass spectrometry

• Project/Area Number: 24619011
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Tokyo Institute of Technology (2015-2017); The Institute of Physical and Chemical Research (2012-2014)
• Principal Investigator: Hongo Yayoi 東京工業大学, 地球生命研究所, 研究員 (40435681)
• Project Period (FY): 2012-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 質量分析 / 分子構造解析 / イオン反応 / イオンモビリティー / イオン解離反応 / イオンモビリティー質量分析 / 機器分析 / 分析化学 / エレクトロスプレーイオン化 / エネルギー分解MS/MS / インドロジテルペン類 / 気相イオン衝突活性化 / 天然物化学 / イオンモビリティー分析

Outline of Final Research Achievements

New molecular structural characterization technique using an energy-resolved ion-mobility mass spectrometry (ER-IMS-MSn) was developed in the period. Data mining for the relationship between stability of the gas phase ion structures and ER-IMS MSn spectra was used to elucidate the structural characterization of cyclic molecules,such as depsipeptides. Ion isomerization during the collision activation experiments was obvious for relatively stable alkaline metal addition ions. So that, alkaline addition ions were suitable for the ion structural characterization.This technique enabled us to obtain additional structure information using mass spectrometry.

Research Products

• [Journal Article] Synthesis of imidazole-activated ribonucleotides using cyanogen chloride (2018)
  • Author(s): Yi, Ruiqin; Hongo, Yayoi; Fahrenbach, Albert C.
  • Journal Title: Chemical Communications Volume: 54 Issue: 5 Pages: 511-514
  • DOI: 10.1039/c7cc08489g
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Estimating the capacity for production of formamide by radioactive minerals on the prebiotic Earth (2018)
  • Author(s): Zachary R. Adam, Yayoi Hongo, H. James Cleaves II, Ruiqin Yi, Albert C. Fahrenbach, Isao Yoda, Masashi Aono
  • Journal Title: Scientific reports Volume: 8 Issue: 1 Pages: 265-265
  • DOI: 10.1038/s41598-017-18483-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Common and potentially prebiotic origin for precursors of nucleotide synthesis and activation, (2017)
  • Author(s): Fahrenbach, AC., Giurgiu, C., Tam, CP., Li, L., Hongo, Y., Aono, M., Szostak, JW.
  • Journal Title: Journal of American Chemical Society: Volume: 139(26) Issue: 26 Pages: 8780-8783
  • DOI: 10.1021/jacs.7b01562
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast. (2016)
  • Author(s): Sun X, Hirai G, Ueki M, Hirota H, Wang Q, Hongo Y, Nakamura T, Hitora Y, Takahashi H, Sodeoka M, Osada H, Hamamoto M, Yoshida M, Yashiroda Y.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 20856-20856
  • DOI: 10.1038/srep20856
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cytoprotective Effects of Lysophospholipids from Sea Cucumber Holothuria atra (2015)
  • Author(s): Nishikawa Y., Furukawa A., Shiga I., Muroi Y., Ishii T., Hongo Y., Takahashi S., Sugawara T., Koshino H., Ohnishi M.
  • Journal Title: PLOS ONE Volume: 10 Issue: 8 Pages: e0135701-e0135701
  • DOI: 10.1371/journal.pone.0135701
  • NAID: 120005763416
  • Peer Reviewed / Open Access
• [Journal Article] RK-270A-C, new oxindole derivatives isolated from a microbial metabolites fraction library of Streptomyces sp. RK85-270. (2015)
  • Author(s): Jang JP, Nogawa T, Uramoto M, Okano A, Futamura Y, Shimizu T, Takahashi S, Jang JH, Ahn JS, Osada H.
  • Journal Title: J Antibiot (Tokyo) Volume: 68 Issue: 4 Pages: 293-295
  • DOI: 10.1038/ja.2014.141
  • Peer Reviewed / Open Access
• [Journal Article] Structural Revision of Kynapcin-12 by Total Synthesis, and Inhibitory Activities against Prolyl Oligopeptidase and Cancer Cells (2014)
  • Author(s): Takahashi S., Yoshida A., Uesugi S., Hongo Y., Kimura K., Matsuoka K. and Koshino H.
  • Journal Title: Bioorganic and Medicinal Chemistry Letters Volume: 24 Issue: 15 Pages: 3373-3376
  • DOI: 10.1016/j.bmcl.2014.05.091
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Structural elucidation and synthesis of vialinin C, a new inhibitor of TNF-α production (2014)
  • Author(s): Ye Y-Q, Negishi C, Hongo Y, Koshino H, Onose J, Abe N, Takahashi S.
  • Journal Title: Bioorg. Med. Chem. Volume: 22 Issue: 8 Pages: 2442-2446
  • DOI: 10.1016/j.bmc.2014.02.058
  • Peer Reviewed
• [Journal Article] RK-1355A and B, novel quinomycin derivatives isolated from a microbial metabolites fraction library based on NPPlot screening. (2014)
  • Author(s): Lim CL, Nogawa T, Uramoto M, Okano A, Hongo Y, Nakamura T, Koshino H, Takahashi S, Ibrahim D, Osada H.
  • Journal Title: J Antibiot (Tokyo) Volume: 67 Issue: 4 Pages: 323-329
  • DOI: 10.1038/ja.2013.144
  • Peer Reviewed
• [Journal Article] Detection of oxygen addition peaks for terpendole E and related indole-diterpene alkaloids in a positive mode ESI-MS (2014)
  • Author(s): Hongo Y., Nakamura T., Takahashi S., Motoyama T., Hayashi T., Hirota H., Osada H., Koshino H.
  • Journal Title: J. Mass Spectrom. Volume: 49 Issue: 6 Pages: 537-542
  • DOI: 10.1002/jms.3360
  • Peer Reviewed / Open Access
• [Presentation] 2018 Y. Hongo, N. Y. Martín, M.Aono, S.heref S. Mansy Effective oligopeptide formations in heat drying (2018)
  • Author(s): Yayoi. Hongo, Noel Yeh. Martín, Masashi.Aono, .Sheref S. Mansy
  • Organizer: 2018 ELSI 6th International Symposium
  • Int'l Joint Research
• [Presentation] Characterization of analogues of amino acids and sugars in radiolysis products using solid phase micro extraction (SPME) -GC-MS (2017)
  • Author(s): Yayoi. Hongo, Kayoko. Yamada, Hans.-J. Hübschmann, Ruiquin. Yi
  • Organizer: Goldschmidt Conference 2017
  • Int'l Joint Research
• [Presentation] Application of Solid Phase Micro Extraction (SPME) and On-Fiber Derivatization (2017)
  • Author(s): Yayoi Hongo, Kayoko Yamada, Hans-J..Hübschmann, Ruiquin Yi
  • Organizer: 日本質量分析学会総合討論会
• [Presentation] 分子・原子を見る目－質量分析－がとらえる地球・生命・化学 (2016)
  • Author(s): 本郷やよい
  • Organizer: 日本分析化学会中部支部 若手交流会・第１６回高山フォーラム
  • Place of Presentation: 高山市図書館「煥章館」(岐阜)
  • Year and Date: 2016-11-11
  • Invited
• [Presentation] イオンモビリティー質量分析法を用いた気相イオン分離と分子構造解析への応用（D2002） (2016)
  • Author(s): 本郷やよい
  • Organizer: 日本分析化学会 第76回分析化学討論会
  • Place of Presentation: 岐阜薬科大学・岐阜大学
  • Year and Date: 2016-05-28
  • Invited
• [Presentation] DART-MSによるモノテルペン類香気の発散追跡 (2015)
  • Author(s): 本郷やよい
  • Organizer: 本質量分析学会 関東談話会
  • Place of Presentation: 日本電子（株）東京支店 大会議室 （東京）
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] フラクションライブラリーとスペクトルデータベースを利用した新規化合物探索 (2015)
  • Author(s): 野川俊彦・ Jang, Jun-pil・ Lim, Chung Liang・ 岡野亜紀子・ 本郷やよい・ 中村健道・ 清水猛・ 高橋俊二・ 長田裕之
  • Organizer: 日本質量分析学会 第６３回質量分析総合討論会
  • Place of Presentation: つくば国際会議場（つくば）
  • Year and Date: 2015-06-16
• [Presentation] 分析結果に見る意外なマススペクトルをイオン化原理から検証する (2015)
  • Author(s): 平岡賢三・本郷やよい
  • Organizer: 日本質量分析学会 第６３回質量分析総合討論会
  • Place of Presentation: つくば国際会議場（つくば）
  • Year and Date: 2015-06-16
  • Invited
• [Presentation] 物質を捉える～質量分析が可能にする研究～ (2015)
  • Author(s): 本郷やよい
  • Organizer: 第81回北陸質量分析談話会
  • Place of Presentation: 福井県立大学福井キャンパス交流センター （福井）
  • Year and Date: 2015-06-06
  • Invited
• [Presentation] DART-MSを用いた発散香気成分のリアルタイム測定 (2015)
  • Author(s): 本郷 やよい
  • Organizer: 2015年第1回TMS研究会(日本質量分析学会）
  • Place of Presentation: 化学会館ホール（東京）
  • Year and Date: 2015-01-23
  • Invited
• [Presentation] DART質量分析による発散香気成分のリアルタイム測定 (2014)
  • Author(s): 本郷やよい、坂倉幹始，塩田晃久，関本奏子
  • Organizer: 第58回 香料・テルペンおよび精油化学に関する討論会
  • Place of Presentation: 和歌山大学
  • Year and Date: 2014-09-22 – 2014-09-24
• [Presentation] Structure and energy dependent ion isomerizations of folates detected using ER-IMS/MSn (2014)
  • Author(s): Yayoi Hongo, Hiroyuki Koshino, Shunya Takahashi, Takemichi Nakamura, Takae Takeuchi
  • Organizer: 20th International Mass Spectrometry Conference
  • Place of Presentation: Geneva, Swaziland
  • Year and Date: 2014-08-24 – 2014-08-29
• [Presentation] 関連化合物との比較解析を用いたブラジル産イソギンチャク由来アシルアミノ酸bunodosine類のタンデム質量分析による構造解析 (2014)
  • Author(s): 本郷やよい、越野広雪、中村健道
  • Organizer: 第62回質量分析総合討論会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] ESI-MS分析で観測されるTerpendoleイオンの酸化とその抑制 (2013)
  • Author(s): 本郷 やよい、中村 健道、高橋 俊哉、本山 高幸、 林 敏明、廣田 洋、長田 裕之、越野 広雪
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学、(宮城県仙台市)
• [Presentation] Energy-resolved ion mobility tandem mass spectrometry: A new approach for probing fragmentation pathways involving ion isomerization (2012)
  • Author(s): Hongo, Y.; Nakamura, T, Takeuchi, T., Takahashi,S., Koshino, H.
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: 京都国際会議場、(京都)
• [Presentation] Ion mobility mass spectrometry of IgGFc glycopeptides from different subclasses (2012)
  • Author(s): Tajiri, M., Hongo, Y.; Nakamura, T, Hirose, K., Wada, N.
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: 京都国際会議場、(京都)
• [Presentation] nergy-resolved ion-mobility tandem mass spectrometry: a new tool for probing gas-phase isomerization and fragmentation of small molecules (2012)
  • Author(s): Hongo, Y.; Nakamura, T, Takeuchi, T.
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: 京都国際会議場、(京都)
• [Presentation] Elucidation of 2-alkylmalonyl-CoA biosynthesis in Reveromycin production
  • Author(s): 宮澤岳、高橋俊二、Suresh Panthee、本郷やよい、Lauren Ray、林輝雄、清水猛、中村健道、Gregory L Challis、 長田裕之
  • Organizer: KRIBB-RIKEN Chemical Biology Joint Symposium
  • Place of Presentation: 2013年5月29日 梧倉、韓国
• [Presentation] Identification of a target for collismycin A, a cytotoxic microbial product, by proteomic profiling
  • Author(s): 川谷誠、井上尭、室井誠、二村友史、青野晴美、浦本昌和、本郷やよい、越野広雪、五十嵐康弘、安藤直子、長田裕之
  • Organizer: AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics
  • Place of Presentation: ボストン、米国
• [Presentation] Isolation of novel bioactive compounds from fraction library of microorganisms based on NPPlot screening
  • Author(s): Lim Chung Liang、野川俊彦、浦本昌和、岡野亜紀子、本郷やよい、中村健道、越野広雪、高橋俊二、
  • Organizer: 第５５回天然有機化合物討論会
  • Place of Presentation: 京都 （同志社大学寒梅館）
• [Presentation] Ion mobility mass spectrometry and MM conformational search of glycopeptides
  • Author(s): 田尻道子、竹内孝江，Fe i f e I Zhu, Maissa M Gaya, 本郷やよい，中村健道，廣瀬賢治，David E Clemmer, 和田芳直
  • Organizer: 第61回質量分析総合討論会
  • Place of Presentation: つくば
• [Presentation] エネルギー分解イオンモビリティー質量分析を用いたイオン異性体の検出と分子構造解析
  • Author(s): 本郷やよい、越野広雪 中村健道
  • Organizer: 第61回質量分析総合討論会
  • Place of Presentation: つくば
• [Presentation] エネルギー分解イオンモビリティー質量分析を用いたイオン異性体の検出と分子構造解析
  • Author(s): 本郷やよい
  • Organizer: 高分子分析研究懇談会第369回例会
  • Place of Presentation: 東京 五反田
  • Invited
• [Presentation] エネルギー分解 イオンモビリティー質量分析法(ER-IMS-MSn)を用いた
  • Author(s): 本郷やよい
  • Organizer: 日本質量分析学会BMS研究会・関東談話会
  • Place of Presentation: 東京 微生物化学研究所
  • Invited
• [Presentation] 精密質量測定と分子式推定
  • Author(s): 本郷やよい
  • Organizer: 理研シンポジウム 物質構造解析2014
  • Place of Presentation: RIKEN, Wako
• [Presentation] Interpretation of MS/MS spectrum including ion isomerization;Characterization of folates using hybrid mass spectrometry
  • Author(s): Yayoi Hongo, Takemichi Nakamura, Shunya Takahashi, Hiroyuki Koshino
  • Organizer: RIKEN&;Max Planck Joint Research Center for Systems Chemical Biology The Second Symposium
  • Place of Presentation: RIKEN, Wako