Development of highly accurate and sensitive quantification method for protein and peptide in biological matrix
| Project/Area Number |
24619012
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KINUMI Tomoya 独立行政法人産業技術総合研究所, 計測標準研究部門, 主任研究員 (90293125)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Yoshihiro 産業技術総合研究所, 健康工学研究部門, グループ長 (10291080)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 質量分析 / 同位体希釈 / タンパク質 / ペプチド / 血清 / 定量分析 / 化学修飾 / C－ペプチド / C反応性タンパク質 / タンパク質定量 |
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| Outline of Final Research Achievements |
Highly accurate and sensitive quantification of serum protein and peptide by mass spectrometry is a key technology which provides the standard value to calibrate and compare the results in clinical chemistry and other research field related to quantification. We performed amino acid analyses of pure C-peptide and C reactive protein with high accuracy, and established certified reference materials of these proteins. To achieve highly accurate and sensitive quantification for serum C-peptide and C reactive protein, we also developed the quantification methods utilizing purification and N-terminal modification methods to enhance the signal intensity.
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Report
(4 results)
Research Products
(14 results)
