Ion mobility mass spectrometry of IgG Fc glycopeptides from different subclasses

• Project/Area Number: 24619014
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Research Institute, Osaka Medical Center for Maternal and Child Health
• Principal Investigator: TAJIRI Michiko 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), その他部局等, 流動研究員 (70581312)
• Co-Investigator(Renkei-kenkyūsha): HONGO Yayoi 独立行政法人理化学研究所, 連携支援ユニット, 専任技師 (40435681) ; NAKAMURA Takemichi 独立行政法人理化学研究所, 連携支援ユニット, 専任研究員 (10360611)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 糖ペプチド / 糖タンパク質 / イオンモビリティー / 質量分析 / 衝突断面積 / 糖鎖－ペプチド間相互作用 / 免疫グロブリンG / 糖鎖-ペプチド間相互作用 / 糖鎖ｰペプチド間相互作用

Outline of Final Research Achievements

Glycosylation alters the physicochemical properties and biological activities of proteins. We analyzed glycopeptides using ion mobility mass spectrometry (IMS MS) and compared the data with the conformations calculated by molecular mechanics (MM) to investigate the interaction between protein and glycan in the vicinity of the attachment site.
In general, the collision cross sections (CCSs) of glycopeptides were smaller than those of peptides. Our findings from IMS MS and MM calculation suggested that the interaction between the innermost glycan and peptide backbone contributes to the compactness of glycopeptide ions in gas phase and the attachment of glycans gives a large influence on the conformation of peptide backbones independent of the glycan structures.

Research Products

• [Presentation] 糖ペプチドのイオンモビリティー質量分析法 (2015)
  • Author(s): 田尻道子、竹内孝江、本郷やよい、中村健道、廣瀬賢治、和田芳直
  • Organizer: 第63回質量分析総合討論会
  • Place of Presentation: 茨城，つくば
  • Year and Date: 2015-06-17 – 2015-06-19
• [Presentation] Ion mobility mass spectrometry and MM conformational search of glycopeptides (2014)
  • Author(s): Michiko Tajiri
  • Organizer: 30th Asilomar Conference on Mass Spectrometry (米国質量分析学会)
  • Place of Presentation: 米国，カリフォルニア，パシフィックグローブ
  • Year and Date: 2014-10-10 – 2014-10-14
  • Invited
• [Presentation] Ion mobility mass spectrometry and MM conformational search of glycopeptides (2014)
  • Author(s): Michiko Tajiri, Takae Takeuchi, Yayoi Hongo, Takemichi Nakamura, Kenji Hirose, Yoshinao Wada
  • Organizer: 第20回質量分析国際会議（IMSC2014）
  • Place of Presentation: スイス，ジュネーブ
  • Year and Date: 2014-08-24 – 2014-08-29
• [Presentation] 糖ペプチドのイオンモビリティー質量分析と分子力学法を用いたコンフォメーション探索 (2014)
  • Author(s): 田尻道子、竹内孝江、本郷やよい、中村健道、廣瀬賢治、和田芳直
  • Organizer: 第62回質量分析総合討論会
  • Place of Presentation: 大阪，吹田
  • Year and Date: 2014-05-14 – 2014-05-16
• [Presentation] Ion mobility mass spectrometry of IgG Fc glycopeptides from different subclasses (2013)
  • Author(s): Michiko Tajiri, Feifei Zhu, Maissa M Gaya, Yoshinao Wada, David E Clemmer
  • Organizer: the 61st. ASMS Conference (American Society for Mass Spectrometry)
  • Place of Presentation: 米国，ミネアポリス
• [Presentation] Ion mobility mass spectrometry of IgG Fc glycopeptides from different subclasses (2013)
  • Author(s): Michiko Tajiri
  • Organizer: 4th Asia Oseania Mass Spectrometry Conference
  • Place of Presentation: 台湾，台北
  • Invited
• [Presentation] 糖ペプチドのイオンモビリティー質量分析と分子力学法を用いたコンフォメーション探索 (2013)
  • Author(s): 田尻道子、竹内孝江、Feifei Zhu、Maissa M Gaya、本郷やよい、中村健道、廣瀬賢治、David E Clemmer、和田芳直
  • Organizer: 第61回質量分析総合討論会
  • Place of Presentation: 茨城，つくば
• [Presentation] Ion mobility mass spectrometry and MM conformational search of glycopeptides (2013)
  • Author(s): Michiko Tajiri, Takae Takeuchi, Yoshinao Wada
  • Organizer: 5th JCS International Symposium on Theoretical Chemistry
  • Place of Presentation: 奈良，奈良
  • Invited
• [Presentation] Ion mobility mass spectrometry of IgG Fc glycopeptides from different subclasses (2013)
  • Author(s): Michiko Tajiri, Feifei Zhu, Maissa M Gaya, Yoshinao Wada, David E Clemmer
  • Organizer: the 61st ASMS Conference (American Society for Mass Spectrometry)
  • Place of Presentation: 米国，ミネアポリス（ミネソタ州）
• [Presentation] Ion mobility mass spectrometry of IgG Fc glycopeptides from different subclasses (2012)
  • Author(s): Michiko Tajiri, Yayoi Hongo, Takemichi Nakamura, Kenji Hirose, Yoshinao Wada
  • Organizer: the 60th ASMS Conference (American Society for Mass Spectrometry)
  • Place of Presentation: カナダ，バンクーバー
• [Presentation] Ion mobility mass spectrometry of IgG Fc glycopeptides from different subclasses (2012)
  • Author(s): Michiko Tajiri, Yayoi Hongo, Takemichi Nakamura, Kenji Hirose, Yoshinao Wada
  • Organizer: The 19th International Mass Spectrometry Conference (IMSC2012)
  • Place of Presentation: 京都