| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
The subject of this study is to establish new method for evaluation of chirality using mass spectrometry only. Chiral hosts were used to detect chirality in mass spectrometry. One enantiomer of the host was labeled with isotope atoms to distinguish each enantiomer in mass spectrometry. Pairs of isotope labeled enantiomer and the other unlabeled enantiomer of hosts such as chiral crown ethers were synthesized to recognize enantiomers of chiral ammonium ions. For free amino acids, labeled/unlabeled enantiomeric pair of copper complex was synthesized. The chiral crown ethers showed high chiral discrimination ability toward chiral ammonium ion guest in FABMS. That ability of the chiral hosts was clarified to depend on the steric structure neighboring the stereo-center of the guests. The correlation between the steric factors of the guests and the results of MS was found in multivariate analysis. Using copper complex, enantiomeric excess of free amino acids was determined using ESIMS.
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