Analysis of bone metabolism in micro- and hypergravity using a novel hard-tissue model

• Project/Area Number: 24620004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命学
• Research Institution: Kanazawa University
• Principal Investigator: SUZUKI Nobuo 金沢大学, 環日本海域環境研究センター, 准教授 (60242476)
• Co-Investigator(Kenkyū-buntansha): KITAMURA Kei-Ichirou 金沢大学, 保健学系, 教授 (80283117) ; HATTORI Atsuhiko 東京医科歯科大学, 教養部, 教授 (70183901) ; IKEGAME Mika 岡山大学, 医歯(薬)学総合研究科, 准教授 (70282986) ; OMORI Katsunori 亜細亜大学, 経済学部, 教授 (20358534) ; 服部 淳彦 東京医科歯科大学, 教養部, 教授 (70183910)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 微小重力 / 過重力 / 骨芽細胞 / 破骨細胞 / 魚類のウロコ / 擬似微小重力 / 魚のウロコ / 3次元クリノスタット / 過重力応答 / 微小重力応答 / 加重力応答

Outline of Final Research Achievements

Because there is no suitable in vitro assay system, the detailed mechanism of bone resorption and formation in gravity has not yet been elucidated. Therefore, we have developed an in vitro assay system and examined bone metabolism using a fish scale that contains osteoblasts and osteoclasts in the calcified tissue. As a result, the scale sensitively responded to gravity. It became clear that this occurred not only at the gene level but also caused morphologic change. This result appears to provide the knowledge that elucidates the mechanism for this response to gravity.

Research Products

• [Journal Article] Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone (2014)
  • Author(s): Yachiguchi K, Sekiguchi T, Nakano M, Hattori A, Yamamoto M, Kitamura K, Maeda M, Tabuchi Y, Kondo T, Kamauchi H, Nakabayashi H, Srivastav AK, Hayakawa K, Sakamoto T, Suzuki N
  • Journal Title: Zoolog Sci Volume: 31 Issue: 5 Pages: 330-337
  • DOI: 10.2108/zs130265
  • NAID: 120005463340
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Increased PGE2 has a positive correlation with plasma calcium during goldfish reproduction. (2014)
  • Author(s): Suzuki, N., Maruyama, Y., Nakano, M., Hattori, A., Honda, M., Shimazaki, Y., Sekiguchi, T., Kamauchi, H., Mishima, H., Wada, S., Srivastav, A.K., Hayakawa, K. and Oshima, Y.
  • Journal Title: J. Fac. Agr., Kyushu Univ. Volume: 59 Pages: 97-101
  • Peer Reviewed
• [Journal Article] Development of Oral Epithelial Cell Line ROE2 with Differentiation Potential from Transgenic Rats Harboring Temperature-Sensitive Simian Virus40 Large T-Antigen Gene (2014)
  • Author(s): Tabuchi Y, Wada S, Ikegame M, Kariya A, Furusawa Y, Hoshi N, Yunoki T, Suzuki N, Takasaki I, Kondo T, Suzuki Y
  • Journal Title: Experimental Animals Volume: 63 Issue: 1 Pages: 31-44
  • DOI: 10.1538/expanim.63.31
  • NAID: 130003391601
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Increased PGE2 has a positive correlation with plasma calcium during goldfish reproduction. (2014)
  • Author(s): Suzuki, N., Maruyama, Y., Nakano, M., Hattori, A., Honda, M., Shimazaki, Y., Sekiguchi, T., Mishima, H., Wada, S., Srivastav, A.K., Hayakawa, K. and Oshima, Y.
  • Journal Title: J. Fac. Agr., Kyushu Univ. Volume: 59
  • Peer Reviewed
• [Journal Article] Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish. (2014)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., Toriba, A., Hayakawa, K., Chowdhury, V.S., Endo, M., Chiba, A., Sekiguchi, T., Nakano, M., Tabuchi, Y., Kondo, T., Wada, S., Mishima, H., Hattori, A. and Suzuki, N.
  • Journal Title: Env. Sci. Poll. Res. Volume: 21
  • NAID: 120005253213
  • Peer Reviewed
• [Journal Article] Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales (2013)
  • Author(s): Yano S, et al. (14番目)
  • Journal Title: Zoological Science Volume: 30 Issue: 3 Pages: 217-223
  • DOI: 10.2108/zsj.30.217
  • Peer Reviewed
• [Journal Article] Zebrafish scales respond differently to in vitro dynamic and static acceleration: analysis of interaction between osteoblasts and osteoclasts. (2013)
  • Author(s): Kitamura, K., Takahira, K., Inari, M., Satoh, Y., Hayakawa, K., Tabuchi, Y., Ohgai, K., Nishiuchi, T., Kondo, T., Mikuni-Takagaki, Y., Chen, W., Hattori, A. and Suzuki, N.
  • Journal Title: Comp. Biochem. Physiol. Part A Volume: 166 Issue: 1 Pages: 74-80
  • DOI: 10.1016/j.cbpa.2013.04.023
  • Peer Reviewed
• [Journal Article] Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish. (2012)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., et al.
  • Journal Title: Env. Sci. Poll. Res. Volume: 12 Issue: 10 Pages: 1347-1355
  • DOI: 10.1007/s11356-012-1347-5
  • NAID: 120005253213
  • Peer Reviewed / Open Access
• [Journal Article] Prostaglandin E2 increases both osteoblastic and osteoclastic activities in the scales of goldfish and participates in the calcium metabolism in goldfish (2012)
  • Author(s): Omori, K., Wada, S., Maruyama, Y., Hattori, A., Kitamura, K., Sato, Y., Nara, M., Funahashi, H., Yachiguchi, K., Hayakawa, K., Endo, M., Kusakari, R., Yano, S., Srivastav, A.K., Kusui, T., Ejiri, S., Chen, W., Tabuchi, Y., Furusawa, Y., Kondo, T., Sasayama, Y. Nishiuchi, T., Nakano, M., Sakamoto, T. and Suzuki, N.
  • Journal Title: Zool. Sci Volume: 29 Issue: 8 Pages: 499-504
  • DOI: 10.2108/zsj.29.499
  • Peer Reviewed
• [Journal Article] Expression of osteoblast-specific genes during spontaneous goldfish scale regeneration and role of cell-to-cell contact in controlling the onset of resorption/regeneration process found in intra-scalepocket autotransplantation of modified scales (2012)
  • Author(s): Thamamongood, T.A, Furuya, R., Fukuba, S., Nakamura, M., Suzuki, N., Hattori, A.
  • Journal Title: Bone Volume: (in press) Issue: 6 Pages: 1240-1249
  • DOI: 10.1016/j.bone.2012.03.021
  • Peer Reviewed
• [Journal Article] Excellent thermal control ability of cell biology experiment facility (CBEF) on ground basis experiments and board experiments in kibo of the international space station. (2012)
  • Author(s): Yano S., et al.
  • Journal Title: Biol. Sci. Space Volume: 26 Pages: 12-20
  • Peer Reviewed
• [Journal Article] Determination of calcium sensing receptor in the scales of goldfish and induction of its mRNA expression by acceleration loading. (2012)
  • Author(s): Kakikawa, M., et al.
  • Journal Title: Biol. Sci. Space Volume: 26 Pages: 26-31
  • Peer Reviewed
• [Journal Article] Osteoblasts and osteoclasts in goldfish regenerating scales respond to mechanical loading: Analysis of osteoblastic and osteoclastic marker mRNA expression. (2012)
  • Author(s): Kitamura, K., et al.
  • Journal Title: Biol. Sci. Space Volume: 26 Pages: 42-46
  • Peer Reviewed
• [Presentation] 宇宙空間における微小重力への金魚再生ウロコ破骨細胞の応答 (2015)
  • Author(s): 池亀美華，田畑 純，服部淳彦，鈴木信雄
  • Organizer: 第120回 日本解剖学会総会･全国学術集会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] 魚類のウロコを用いた宇宙生物学的研究：キンギョのウロコ及び骨疾患モデルラットの骨代謝に対するブロモメラトニンの新規作用 (2015)
  • Author(s): 鈴木信雄，矢野幸子，大森克徳，北村敬一郎，清水宣明，西内 巧，染井正徳，関口俊男，渡辺良成，池亀美華，近藤 隆，田渕圭章，鈴木 徹，遠藤雅人，竹内俊郎，江尻貞一，三島弘幸，嶋津 徹、関 あずさ，舟橋久幸，高垣裕子，笠原春夫，永瀬 睦，田谷敏貴，長野慎太郎，宮下知之，服部淳彦
  • Organizer: 第29回宇宙環境利用シンポジウム
  • Place of Presentation: 宇宙航空研究開発機構 宇宙科学研究所 相模原キャンパス
  • Year and Date: 2015-01-24 – 2015-01-25
• [Presentation] 骨モデル（魚のウロコ）を用いて宇宙実験：骨を壊す細胞の変化． (2014)
  • Author(s): 鈴木信雄
  • Organizer: 平成26年度日本動物学会中部支部大会、公開講演会
  • Place of Presentation: コンセール能登
  • Year and Date: 2014-11-25
  • Invited
• [Presentation] 破骨細胞及び骨芽細胞の重力応答：骨モデル（ウロコ）を用いた解析 (2014)
  • Author(s): 山本 樹，池亀美華，田渕圭章，矢野幸子，遠藤雅人，近藤 隆，北村敬一郎，関口俊男，服部淳彦，鈴木信雄
  • Organizer: 平成26年度日本動物学会中部支部大会
  • Place of Presentation: のと勤労者プラザ
  • Year and Date: 2014-11-24
• [Presentation] 走査型及び透過型電子顕微鏡によるキンギョウロコの収納ポケットの形態観察 (2014)
  • Author(s): 舟橋久幸，鬼木弘明，赤塚涼佑，耿啓達，鈴木信雄，服部淳彦
  • Organizer: 第46回日本臨床分子形態学会
  • Place of Presentation: 日本大学
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] 過重力及び擬似微小重力に対する破骨細胞及び骨芽細胞の応答解析：骨モデル（ウロコ）を用いた解析 (2014)
  • Author(s): 山本 樹，池亀美華，田渕圭章，矢野幸子，遠藤雅人，近藤 隆，北村敬一郎，関口俊男，服部淳彦，鈴木信雄
  • Organizer: 日本宇宙生物科学会第28回大会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2014-09-22 – 2014-09-23
• [Presentation] 宇宙実験を基盤にした骨疾患治療薬の開発 (2014)
  • Author(s): 鈴木信雄，関あずさ，高垣裕子，田渕圭章，矢野幸子，北村敬一郎，関口俊男，池亀美華，近藤 隆，染井正徳，服部淳彦
  • Organizer: 日本宇宙生物科学会第28回大会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2014-09-22 – 2014-09-23
• [Presentation] Response of osteoclasts and receptor activator of nuclear factor kappa-B ligand expression in the regenerating scales of goldfish under microgravity. (2014)
  • Author(s): Ikegame, M., Hattori, A., Tabuchi, Y., Kitamura, K., Yamamoto, T., Nakano, M., Yano, S., Yamamoto, T. And Suzuki, N.
  • Organizer: 2nd International Symposium on Mechanobiology
  • Place of Presentation: Ｏｋａｙａｍａ Ｕｎｉｖ．
  • Year and Date: 2014-05-20 – 2014-05-23
  • Invited
• [Presentation] 超音波による機械的刺激の骨芽細胞及び破骨細胞に対する作用：魚のウロコを用いた解析． (2014)
  • Author(s): 鈴木信雄，北村敬一郎，古澤之裕，田渕圭章，近藤 隆，池亀美華，清水宣明，和田重人，服部淳彦
  • Organizer: 日本超音波医学会第87回学術集会
  • Place of Presentation: 横浜国際会議場
  • Year and Date: 2014-05-09 – 2014-05-11
  • Invited
• [Presentation] Development and application of a fish scale in vitro assay system. (2013)
  • Author(s): Suzuki, N.
  • Organizer: The 9th International Satellite Symposium on the Comparative Endocrinology of Calcium Regulation
  • Place of Presentation: 神戸国際会議場（神戸市、兵庫県）
  • Invited
• [Presentation] キンギョウロコのアパタイトの解析－赤外ラマン分光によるアプローチ． (2013)
  • Author(s): 奈良雅之，服部淳彦，大西晃宏，赤塚陽子，矢野幸子，鈴木信雄，松田准一
  • Organizer: 第22回日本バイオイメージング学会
  • Place of Presentation: 東京大学薬学部（東京都）
• [Presentation] 微小重力におけるウロコの破骨細胞の応答． (2013)
  • Author(s): 鈴木信雄，池亀美華，山本 樹，北村敬一郎，田渕圭章，矢野幸子，服部淳彦
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山大学（岡山県）
• [Presentation] 擬似微小重力に対する骨モデル（ウロコ）の破骨細胞及び骨芽細胞の応答解析． (2013)
  • Author(s): 山本 樹，池亀美華，田渕圭章，矢野幸子，遠藤雅人，近藤 隆，中野真樹，北村敬一郎，関口俊男，清水宣明，服部淳彦，鈴木信雄
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県）
• [Presentation] ウロコを用いた宇宙実験 (2012)
  • Author(s): 鈴木信雄
  • Organizer: 宇宙メラトニン研究会
  • Place of Presentation: 金沢大学、石川県
• [Presentation] 宙空間におけるウロコの破骨細胞の形態及び細胞活性の変化． (2012)
  • Author(s): 鈴木信雄，池亀美華，北村敬一郎，矢野幸子，服部淳彦
  • Organizer: 平成24年度日本動物学会中部支部例会
  • Place of Presentation: 信州大学、長野県
• [Presentation] 骨モデル（魚のウロコ）に対する宇宙放射線（重粒子線）の影響． (2012)
  • Author(s): 上西篤志，丸山雄介，中野真樹，松本謙一郎，大森克徳，田渕圭章，和田重人，近藤 隆， 遠藤雅人，北村敬一郎，早川和一，清水宣明，関口俊男，服部淳彦，鈴木信雄
  • Organizer: 平成24年度日本動物学会中部支部例会
  • Place of Presentation: 信州大学、長野県
• [Presentation] 新規メラトニン誘導体－骨粗鬆症の治療に向けて．
  • Author(s): 鈴木信雄，関 あずさ，関口俊男，染井正徳，高垣裕子，矢野幸子，服部淳彦
  • Organizer: 第5回抗加齢内分泌研究会
  • Place of Presentation: 立教大学（東京都）
  • Invited
• [Presentation] キンギョのウロコを使って宇宙実験：宇宙に行くと骨を壊す細胞はどのように変化するのか？
  • Author(s): 鈴木信雄
  • Organizer: 動物学会関東支部公開講演会
  • Place of Presentation: 東京医科歯科大学、東京都
  • Invited