Study of space radiation using ES cells
Project/Area Number |
24620007
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
宇宙生命学
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Research Institution | Osaka City University |
Principal Investigator |
MORITA Takashi 大阪市立大学, 医学(系)研究科(研究院), 教授 (70150349)
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Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Kayo 大阪市立大学, 医学(系)研究科(研究院), 准教授 (30311921)
KIZU Akane 大阪市立大学, 医学(系)研究科(研究院), 助教 (30623201)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 宇宙放射線 / ES細胞 / 染色体異常 / 国際宇宙ステーション / DNA20差鎖切断 / ヒストンH2AX / 遺伝子発現誘導 / Tet-0n / 宇宙 / 重粒子線 / ES細胞 / 発生 / 哺乳動物細胞 / アメリカ合衆国 / NASA 共同研究 |
Outline of Final Research Achievements |
It is important to estimate the influence of space radiation on the human body during long stays in space including missions to Internatonal Space Station (ISS), the Earth’s moon, or Mars. In order to protect space radiation, we investigate the effect of stimulation of DNA repair genes, such as histone H2AX. We made plasmid vector using Tet-on inducing system to produce histone H2AX. The vector was introduced in ES cells and H2AX gene was induced to express by Doxycyline addition. It resulted in up-regulation of H2AX gene expression and increased number of foci of γ-H2AX foci after irradiation.
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Report
(4 results)
Research Products
(10 results)
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[Presentation] The effects of space radiation to mouse embryonic stem cells in international space station (Stem Cells)2014
Author(s)
K. Yoshida, A. Kizu, K. Kitada, M. Hada2, K., Eguchi-Kasai, T. Teramura, H. Suzuki, T. Shimazu, S. Yano, M. Shirakawa, I. Osada, H. Kasahara, F. A. Cucinotta, T. Morita
Organizer
60th Annual International Meeting Radiation Research Society
Place of Presentation
Las Vegas,NV (USA)
Year and Date
2014-06-21 – 2014-06-24
Related Report
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[Presentation] Detection of Chromosome Aberration by using Histone H2AX-deficient2013
Author(s)
Yoshida, K.,Hada, Ml, Eguchi-Kasai, K., Teramura, T., Cucinotta, F.A., Morita, T.
Organizer
Heavy Ion in Therapy and Space Radiation Symposium 2013
Place of Presentation
千葉、京葉銀行文化会館
Related Report
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