Radiation effects on the relation between oxidative stress and the neuronal development and function

• Project/Area Number: 24620012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命学
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: SAKASHITA Tetsuya 独立行政法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究員 (30311377)
• Co-Investigator(Kenkyū-buntansha): YANASE Sumino 大東文化大学, スポーツ健康科学部, 准教授 (90249061) ; SUZUKI Michiyo 独立行政法人日本原子力研究開発機構, 原子力科学研究部門・量子ビーム応用研究センター, 研究員 (10507437)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 宇宙放射線 / 神経回路 / 学習行動 / モデル生物 / 酸化ストレス / 宇宙放射線生物学

Outline of Final Research Achievements

To achieve the inter-planetary space mission, e.g. Mars mission, it is important to investigate the effects of space radiation exposure on the life system. We aimed to clarify the radiation effects on the relation between oxidative stress and the neuronal function, i.e. learning or behavior. First, we examined the radiation effects on the learning behavior along to the development, resulting on the maximum effects on the adult but early larvae. Next, we tried to the microbeam irradiation to reveal the sensitive area of the nervous network in C. elegans. As a result, we found that the whole body of C. elegans was sensitive to the radiation exposure on learning behavior.

Research Products

• [Journal Article] Detection and meas-urement of abnormal posture under stress-ful conditions in nematode C. elegans. (2014)
  • Author(s): K. Ide, T. Ishikawa, S. Arai, T. Morioka, H. Kaneda, M. Suzuki, T. Sakashita, N. Ishii, S. Yanase
  • Journal Title: C. elegans Topic Meeting: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs 2014 Volume: なし Pages: 95-95
  • Peer Reviewed
• [Journal Article] <i>PAK1</i>-deficiency/down-regulation reduces brood size, activates <i>HSP16.2</i> gene and extends lifespan in <i>Caenorhabditis elegan</i><i>s </i> (2013)
  • Author(s): Yanase S, Luo Y, Maruta H
  • Journal Title: Drug Discoveries & Therapeutics Volume: 7 Issue: 1 Pages: 29-35
  • DOI: 10.5582/ddt.2013.v7.1.29
  • NAID: 130005054758
  • ISSN: 1881-7831, 1881-784X
  • Peer Reviewed
• [Journal Article] Behavioral Resistance of Caenorhabditis elegans Against High-LET Radiation Exposure (2012)
  • Author(s): Sakashita T, Suzuki M, Hamada N, Shimozawa Y, Shirai-Fukamoto K, Yokota Y, Hamada-Sora S, Kakizaki T, Wada S, Funayama T, Kobayashi Y
  • Journal Title: Biol Sci Space Volume: 26 Pages: 7-11
  • NAID: 130004450620
  • Peer Reviewed
• [Journal Article] Innate immune genes including a mucin-like gene, mul-1, induced by ionizing radiation in Caenorhabditis elegans (2012)
  • Author(s): Kimura T, Takanami T, Sakashita T, Wada S, Kobayashi Y, Higashitani A
  • Journal Title: Radiat Res Volume: 178(4) Issue: 4 Pages: 313-20
  • DOI: 10.1667/rr2989.1
  • Peer Reviewed
• [Journal Article] Effects of Low- and High-LET Radiation on the Salt Chemotaxis Learning in Caenorhabditis elegans (2012)
  • Author(s): Sakashita T, Suzuki M, Hamada N, Shimozawa Y, Shirai-Fukamoto K, Yokota Y, Hamada-Sora S, Kakizaki T, Wada S, Funayama T, Kobayashi Y
  • Journal Title: Biol Sci Space Volume: 26 Pages: 21-25
  • NAID: 130004450622
  • Peer Reviewed
• [Journal Article] Timing mechanism and effective activation energy concerned with aging and lifespan in the long-lived and thermosensory mutants of Caenorhabditis elegans (2012)
  • Author(s): Suda H, Sato K, Yanase S
  • Journal Title: Mechanisms of Ageing & Development Volume: 133 Pages: 600-610
  • Peer Reviewed
• [Presentation] Responses of the Salt Chemotaxis Learning in Wild Type and Mutant C. elegans to Microbeam Irradiation (2015)
  • Author(s): Sakashita T, Suzuki M, Hattori Y, Ikeda H, Mutou Y, Yokota Y, Funayama T, Hamada N, Shirai K, Kobayashi Y
  • Organizer: The Fifth International Society of Radiation Neurobiology Conference
  • Place of Presentation: 高崎シティギャラリー（高崎市，日本）
  • Year and Date: 2015-02-21
• [Presentation] マイクロビーム照射に対する線虫変異体の化学走性学習の応答 (2014)
  • Author(s): 坂下哲哉, 鈴木芳代, 服部佑哉, 池田裕子, 武藤泰子, 横田裕一郎, 舟山知夫, 浜田信行, 白井花菜, 小林泰彦, 浜田信行, 白井花菜
  • Organizer: 第9回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎シティギャラリー（高崎市，日本）
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] 線虫の筋運動に対する炭素イオンマイクロビーム局部照射の影響 (2014)
  • Author(s): 鈴木 芳代，坂下 哲哉，服部 佑哉，小林 泰彦
  • Organizer: 第9回高崎量子応用研究シンポジウム要旨集
  • Place of Presentation: 高崎シティギャラリー（高崎市，日本）
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] 線虫の行動における生存戦略について ―走性に着目して― (2014)
  • Author(s): 坂下哲哉
  • Organizer: 日本放射線影響学会第57回大会
  • Place of Presentation: かごしま県民交流センター（鹿児島市，日本）
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] 放射線による線虫の運動抑制における放射線産生ROSの関与 (2014)
  • Author(s): 鈴木 芳代，坂下 哲哉，服部 佑哉，小林 泰彦
  • Organizer: 日本放射線影響学会第57回大会
  • Place of Presentation: かごしま県民交流センター（鹿児島市，日本）
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] 線虫変異体の化学走性学習に対する重イオンマイクロビーム局部照射の影響 (2014)
  • Author(s): 坂下哲哉, 鈴木芳代, 服部佑哉, 池田裕子, 武藤泰子, 横田裕一郎, 舟山知夫, 浜田信行, 白井花菜, 小林泰彦
  • Organizer: 日本宇宙生物科学会第28回大会
  • Place of Presentation: 大阪府立大学（堺市，日本）
  • Year and Date: 2014-09-22 – 2014-09-23
• [Presentation] Oxidative stress suppresses locomotion and pumping motion in Caenorhabditis elegans. (2014)
  • Author(s): Michiyo Suzuki, Tetsuya Sakashita, Yuya Hattori, Yasuhiko Kobayashi
  • Organizer: 2014 C. elegans Development, Cell Biology, and Gene Expression Topic Meeting in association with The 6th Asia-Pacific C. elegans Meeting
  • Place of Presentation: 奈良県新公会堂（奈良市，日本）
  • Year and Date: 2014-07-15 – 2014-07-19
• [Presentation] Detection and meas-urement of abnormal posture under stress-ful conditions in nematode C. elegans. (2014)
  • Author(s): K. Ide, T. Ishikawa, S. Arai, T. Morioka, H. Kaneda, M. Suzuki, T. Sakashita, N. Ishii, S. Yanase
  • Organizer: C. elegans Topic Meeting: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs
  • Place of Presentation: ウィスコンシン大学（マディソン市，米国）
  • Year and Date: 2014-07-10 – 2014-07-13
• [Presentation] Exploration of the site responsible for the radiation response of the salt chemotaxis learning in C. elegans using heavy-ion microbeam (2013)
  • Author(s): Sakashita T, Suzuki M, Mutou Y, Hattori Y, Ikeda H, Yokota Y, Funayama T, Hamada N, Fukamoto K, Kobayashi Y
  • Organizer: Heavy Ion in Therapy and Space Radiation Symposium 2013
  • Place of Presentation: 京葉銀行文化プラザ（千葉市, 日本）
• [Presentation] マイクロビーム照射に対するMock条件付け線虫の化学走性の応答 (2013)
  • Author(s): 坂下哲哉, 下澤容子, 鈴木芳代, 服部佑哉, 池田裕子, 武藤泰子, 横田裕一郎, 舟山知夫, 小林泰彦, 浜田信行, 白井花菜
  • Organizer: 第8回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎シティギャラリー（高崎市, 日本）
• [Presentation] Intermittent hyperoxia-induced hormesis decreases aerobic respiration via ins/IGF-1 and p53/CEP-1 signalings in C. elegans (2013)
  • Author(s): Yanase S, Shoyama T, Suda H, Ishii N
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: University of California（ロサンゼルス, 米国）
• [Presentation] Cell-level modeling and simulation of the pharyngeal pumping in Caenorhabditis elegans (2013)
  • Author(s): Hattori Y, Suzuki M, Tsuji T, Kobayashi Y
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: University of California（ロサンゼルス, 米国）
• [Presentation] マイクロビームを用いた線虫の化学走性学習に及ぼす放射線影響部位の探索 (2013)
  • Author(s): 坂下哲哉, 鈴木芳代, 服部佑哉, 池田裕子, 武藤泰子, 横田裕一郎, 舟山知夫, 浜田信行, 深本花菜, 小林泰彦
  • Organizer: 第３回国際放射線神経生物学会大会
  • Place of Presentation: 万国津梁館 （名護市, 日本）
• [Presentation] マイクロビームを用いた線虫の化学走性学習に及ぼす放射線影響部位の探索 (2012)
  • Author(s): 坂下哲哉, 鈴木芳代, 服部佑哉, 池田裕子, 武藤泰子, 横田裕一郎, 舟山知夫, 浜田信行, 深本花菜, 小林泰彦
  • Organizer: 日本放射線影響学会第55回大会
  • Place of Presentation: 東北大学 （仙台市, 日本）
• [Presentation] 線虫の行動に観られる高LET放射線耐性 (2012)
  • Author(s): 坂下哲哉, 鈴木芳代, 浜田信行, 下澤容子, 白井花菜, 横田裕一郎, 浜田桜, 柿崎竹彦, 和田成一, 舟山知夫, 小林泰彦
  • Organizer: 第26回日本宇宙生物科学会学術集会
  • Place of Presentation: 阿波観光ホテル （徳島市, 日本）
• [Presentation] Computational inferences on neurotransmission in chemotaxis learning in Caenorhabditis elegans (2012)
  • Author(s): Michiyo Suzuki, Tetsuya Sakashita, Yasuhiko Kobayashi, Toshio Tsuji
  • Organizer: EMBO Conference Series: C. elegans Neurobiology
  • Place of Presentation: EMBL （Heidelberg, Germany）
• [Presentation] Computational study of neurotransmission in chemotaxis of Caenorhabditis elegans (2012)
  • Author(s): Michiyo Suzuki, Tetsuya Sakashita, Toshio Tsuji, Yasuhiko Kobayashi
  • Organizer: Foundations of Systems Biology in Engineering (FOSBE) 2012
  • Place of Presentation: 慶応大学鶴岡キャンパス （鶴岡市, 日本）
• [Presentation] Supplemental cellular protection by a carotenoid astaxanthin extends lifespan via ins/IGF-1 signaling in C. elegans
  • Author(s): Yazaki K, Yoshikoshi C, Oshiro S, Takahashi J, Yanase S
  • Organizer: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting 2012
  • Place of Presentation: UW Madison （WI, USA）
• [Presentation] Supplemental cellular protection by a marine carotenoid extends lifespan via ins/IGF-1 signaling in nematode C. elegans
  • Author(s): Yazaki K, Yoshikoshi C, Oshiro S, Takahashi J, Yanase S
  • Organizer: BIT’s 1st Annual International Congress of Marine Algae-2012
  • Place of Presentation: World Expo Center （Dalian, China）
• [Book] PAKs, RAC/CDC42(p21)-activated kinases: Towards the cure of cancer and other PAK-dependent diseases (edited by Hiroshi Maruta) Chapter 7: PAK1 controls the lifespan (2013)
  • Author(s): S. Yanase, H. Maruta
  • Publisher: Elsevier