| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
In this study, I developed a new system recording the gene expression in multiple areas of the freely moving mice with analyzing the body temperature and sleep-awake rhythm. In addition, new program to analyze the sleep-awake by tracing technique of the target area in freely moving mice was added into the system. Using this system, I succeeded in visualizing the internal desynchronization in freely moving mice. There were two type of tissues to express the phase shifts of clcok gene expression. One showed rapid phase shift with the same speed of that of locomotor activity rhythm, the others showed slow phase shifts. Present system is a powerful system for analyzing many areas of biomedical research, enabling simultaneous long term tracking of behavior and gene expression in multiple tissues.
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