Novel in vivo imaging with the real time tracking system
| Project/Area Number |
24621001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
睡眠科学
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| Research Institution | Hokkaido University |
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Principal Investigator |
HAMADA Toshiyuki 北海道大学, 医学(系)研究科(研究院), 特任准教授 (20360208)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 時計遺伝子 / 行動リズム / 光バイオイメージング / ルシフェラーゼ / ルシフェリン / 体内時計 / 時計遺伝子発現 / 睡眠 |
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| Outline of Final Research Achievements |
In this study, I developed a new system recording the gene expression in multiple areas of the freely moving mice with analyzing the body temperature and sleep-awake rhythm. In addition, new program to analyze the sleep-awake by tracing technique of the target area in freely moving mice was added into the system. Using this system, I succeeded in visualizing the internal desynchronization in freely moving mice. There were two type of tissues to express the phase shifts of clcok gene expression. One showed rapid phase shift with the same speed of that of locomotor activity rhythm, the others showed slow phase shifts. Present system is a powerful system for analyzing many areas of biomedical research, enabling simultaneous long term tracking of behavior and gene expression in multiple tissues.
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Report
(4 results)
Research Products
(13 results)
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[Presentation] 浜田俊幸, 石川正純, Kenneth Sutherland, 宮本直樹, 白土博樹, 本間さと, 本間研一2014
Author(s)
浜田俊幸, 石川正純, Kenneth Sutherland, 宮本直樹, 白土博樹, 本間さと, 本間研一
Organizer
第２０回日本時間生物学会学術大会
Place of Presentation
九州大学「福岡県・福岡市」
Year and Date
2014-11-09 – 2014-11-10
Related Report
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