| Project/Area Number |
24650157
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Komiya Ken 東京工業大学, 総合理工学研究科(研究院), 助教 (20396790)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | DNAコンピュータ / DNAナノテクノロジー / ナノマシン / ナノ反応場 / ニッキング酵素 / DNAウォーカ / DNAzyme / ＤＮＡコンピュータ / 生体生命情報学 / 知能ロボティックス / 高分子合成 |
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| Outline of Final Research Achievements |
In this research project, construction of a dynamical reaction field at a nano-meter scale was investigated by taking advantage of an autonomous walking device, consisting of a set of DNA strands and an enzyme, for achieving autonomous implementation of a multi-step polymerization reaction. We revealed the optimal length of DNA sequences and the appropriate enzymes that could improve the walking performance of the device, via absorbance measurement, fluorescence measurement and electrophoresis. In addition, we revealed the use of DNAzyme is preferable for improving the efficiency and flexibility in synthesis reaction. Currently, construction of a programmable and dynamical reaction field at a nano-meter scale is under further investigation by integrating the obtained knowledge.
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