| Project/Area Number |
24650159
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | Keio University |
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Principal Investigator |
OKA KOTARO 慶應義塾大学, 理工学部, 教授 (10276412)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 線虫 / 神経回路 / 特定神経細胞破壊 / 細胞膜電位計測 / 細胞カルシウム計測 / マイクロ流体デバイス / 細胞内カルシウム計測 / マイクロデバイス / カルシウムイメージング / 微小環境制御 / 匂い刺激 / 行動観察 / 膜電位イメージング / 単一神経細胞情報処理 / 神経細胞破壊 |
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| Research Abstract |
Caenorhabditis elegans is known as one of model animals, and it has been used for understanding the relationship between neural functions and behaviors. The worm senses odors and shows taxis toward the source of favorite odor by detecting its concentration gradient. However, little is known how exactly worms can discriminate the concentration of odor and change their head turning behavior in regard to the response of olfactory neurons. For demonstrating the role of single neurons in specific behaviors, we investigated the neural activity from a single neuron by Ca and membrane potential imaging, and also quantitative behaviors by developing new types of micro-fluidic devices. We succeeded to visualize the Ca oscillation synchronized with the head movement during odor stimulation in AIY interneuron.
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