| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The nervous system is structurally and functionally asymmetric along the midline. However, the mechanism underlying the formation of the left-right brain asymmetry is largely unknown. We previously reported that the filopodia of the growth cones at the tips of the neurites autonomously rotate in the direction of the right-handed screw by way of myosin V and suggested that the filopodial rotation could trigger the formation of brain asymmetry. Here we hypothesized that the filopodial rotation originates in the chiral molecular structure of myosin V. To test this hypothesis, we developed the methods of molecular manipulation of the chiral structure of myosin V. To quantitatively analyze the effect of the manipulated myosin V on the filopodial motility, we further developed an efficient method of 3D time-lapse imaging with differential interference contrast microscopy.
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