Establishing a new method to analyze neuronal activities of all individual neurons of C. elegans CNS.

• Project/Area Number: 24650167
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: Kyushu University
• Principal Investigator: ISHIHARA Takeshi 九州大学, 理学(系)研究科(研究院), 教授 (10249948)
• Co-Investigator(Renkei-kenkyūsha): TERAMOTO Takayuki 九州大学, 大学院理学研究院, 准教授 (90571836) ; KUGE Sayuri 九州大学, 大学院理学研究院, 学術研究員 (50260104)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 神経科学 / イメージング / 線虫 / 情報処理

Outline of Final Research Achievements

To visualize the informational processing in CNS, we planned to device a method that enable us to analyze the activities of all individual neurons in living C. elegans. To do this, we improved a microscopy system, optimized Ca2+ probe and image processing including tracking neurons by pattern matching. By using this system, we succeeded in evaluating more than 20 individual neurons and in observing a synchronous activity.

Research Products

• [Journal Article] Automated detection and tracking of many cells by using 4D live-cell imaging data (2014)
  • Author(s): Terumasa Tokunaga, Osamu Hirose, Shotaro Kawaguchi, Yu Toyoshima, Takayuki Teramoto, Hisaki Ikebata, Sayuri Kuge, Takeshi Ishihara, Yuichi Iino, Ryo Yoshida
  • Journal Title: Bioinformatics Volume: 5;30(12) Issue: 12 Pages: 43-51
  • DOI: 10.1093/bioinformatics/btu271
  • Peer Reviewed / Open Access
• [Journal Article] 神経系の丸ごと観測による神経回路の解析 (2014)
  • Author(s): 飯野雄一・寺本孝行・石原健
  • Journal Title: 生体の科学 Volume: 65 Pages: 416-417
• [Journal Article] Forgetting in C. elegans is accelerated by neuronal communication via the TIR-1/JNK-1 pathway. (2013)
  • Author(s): Inoue A, Sawatari E, Hisamoto N, Kitazono T, Teramoto T, Fujiwara M, Matsumoto K, Ishihara T.
  • Journal Title: Cell Reports Volume: 3(3) Issue: 3 Pages: 808-819
  • DOI: 10.1016/j.celrep.2013.02.019
  • Peer Reviewed
• [Journal Article] Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour (2012)
  • Author(s): Takayuki Uozumi, Takaaki Hirotsu, Kazushi Yoshida, Ryuji Yamada, Akiya Suzuki, Gun Taniguchi, Yuichi Iino & Takeshi Ishihara
  • Journal Title: Scientific Reports Volume: 2 Issue: 1 Pages: 500-500
  • DOI: 10.1038/srep00500
  • Peer Reviewed
• [Journal Article] Odour concentration-dependent olfactory preference change in C.elegans (2012)
  • Author(s): Kazushi Yoshida
  • Journal Title: Nature Communications Volume: 3 Issue: 1 Pages: 739-739
  • DOI: 10.1038/ncomms1750
  • Peer Reviewed
• [Presentation] 線虫中枢神経系のまるごと計測および定量解析に向けた取り組み (2015)
  • Author(s): 寺本孝行、 吉田亮
  • Organizer: 定量生物学の会第七回年会
  • Place of Presentation: 福岡
  • Year and Date: 2015-01-11 – 2015-01-12
  • Invited
• [Presentation] 線虫、C. elegansの単一神経細胞におけるカルシウムダイナミクス (2014)
  • Author(s): 久下(原) 小百合、寺本 孝行、 石原 健
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] Active forgetting of the olfactory memory in C.elegans. (2014)
  • Author(s): Takeshi Ishihara,Akitoshi Inoue, Etsuko Sawatari, Tomohiro Kitazono, Manabi Fujiwara, Takayuki Teramoto
  • Organizer: NEUROBIOLOGY:DIVERSE SPECIES & CONSERVED PRINCIPLES.
  • Place of Presentation: 中国 蘇州
  • Year and Date: 2014-09-16 – 2014-09-20
  • Invited
• [Presentation] Tracking of Ca2+ dynamics in a whole single neuron (2014)
  • Author(s): Sayuri Kuge, Takayuki Teramoto, Takeshi Ishihara
  • Organizer: 2014 C. elegans topic meeting: Neuronal Development, Synaptic Function and behavior
  • Place of Presentation: 米国 マディソン
  • Year and Date: 2014-07-07 – 2014-07-09
• [Presentation] 4-D Ca2+ imaging of the multiple neurons in a local circuit regulating behavioral choice (2013)
  • Author(s): Takayuki Teramoto, Yuta Yamamoto, and Takeshi Ishihara
  • Organizer: 19th International C.elegans Meeting
  • Place of Presentation: ロサンジェルス アメリカ合衆国
• [Presentation] C. elegansの中枢神経系まるごとのカルシウムイメージングに向けて (2013)
  • Author(s): 寺本 孝行、石原 健
  • Organizer: 包括的脳科学研究推進支援ネットワーク 平成25 年度 夏のワークショップ
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] 4-D Ca2+ imaging of the multiple neurons that regulate the sensory integration underlying behavioral choice (2012)
  • Author(s): Takayuki Teramoto
  • Organizer: EMBO Conference Series C.elegans Neurobiology
  • Place of Presentation: EMBL Heidelberg, Germany
• [Presentation] 線虫の行動選択を制御する神経回路とその活動の可視化 (2012)
  • Author(s): 石原 健
  • Organizer: 文部科学省新学術領域研「システム分子行動学」公開班会議
  • Place of Presentation: 仙台
• [Presentation] 線虫C. elegansにおける３次元構成画像の解析に基づく多数の神経活動の同時測定 (2012)
  • Author(s): 石原 健
  • Organizer: バイオイメージ・インフォマティクス ワークショップ2012
  • Place of Presentation: 神戸
• [Presentation] 4-D Ca2+イメージングによる線虫C. elegansの行動選択を制御する複数ニューロンの活動の可視化と計測 (2012)
  • Author(s): 寺本 孝行
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Invited
• [Remarks] 九州大学理学研究院生物科学部門分子遺伝学研究室
  • URL: http://www.biology.kyushu-u.ac.jp/~bunsiide/
• [Remarks] 九州大学分子遺伝学研究室
  • URL: http://www.biology.kyushu-u.ac.jp/~bunsiide/