Development of a novel technique for the trans-synaptic gene manipulation

• Project/Area Number: 24650175
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: FUJIMOTO Satoshi 独立行政法人理化学研究所, 発生・再生科学総合研究センター, 研究員 (50586592)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 神経回路形成 / マウス / 遺伝子改変

Research Abstract

Neural circuits are established by specific neuronal connections via synapses. Therefore, gene manipulation system in a circuit level could be a powerful tool to understand the structural basis of neural computation. In this study, we attempted to make a novel genetically-encoded protein that enables us to trans-synaptically visualize and manipulate a specific neural circuit.

Research Products

• [Journal Article] Optical Clearing Using SeeDB (2014)
  • Author(s): Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Journal Title: Bio-protocol Volume: ISSN :2331-8325
  • URL: http://www.bio-protocol.org/wenzhang.aspx?id=1042
  • Peer Reviewed
• [Journal Article] SeeDB : a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Ke M-T, Fujimoto S, Imai T
  • Journal Title: Nature Neuroscience Volume: 16 Issue: 8 Pages: 1154-1161
  • DOI: 10.1038/nn.3447
  • Peer Reviewed
• [Presentation] SeeDB—A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: 43rd Annual Meeting Society for Neuroscience, November 9-13.2013
  • Place of Presentation: San Diego, California, USA
• [Presentation] SeeDB—A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: 国際シンポジウムOptogenetics2013
  • Place of Presentation: 東京(慶應義塾大学三田キャンパス北館ホール)
• [Presentation] SeeDB—A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: ECRO2013
  • Place of Presentation: Leuven, Belgium
• [Presentation] SeeDB—A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: Cold Spring Harbor Laboratory on Wiring the Brain
  • Place of Presentation: NY, USA
• [Presentation] SeeDB—A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: The 6th International Neural Microcircuit Conference Functional Mechanism of Cortical Microcircuit
  • Place of Presentation: 愛知県岡崎市(自然科学研究機構岡崎コンファレンスセンター)
• [Presentation] SeeDB : an aqueous optical clearing agent for imaging intact fluorescence and morphology in the mouse brain (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: 島根県松江市(くにびきメッセ)
• [Presentation] SeeDB: an aqueous optical clearing agent for imaging intact fluorescence and morphology in the mouse brain. (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: くにびきメッセ（島根県松江市）
• [Presentation] SeeDB―A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: The 6th International Neural Microcircuit Conference Functional Mechanism of Cortical Microcircuit
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター（愛知県岡崎市）
• [Presentation] SeeDB―A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: Cold Spring Harbor Laboratory on Wiring the Brain
  • Place of Presentation: ニューヨーク州、アメリカ
• [Presentation] SeeDB―A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: ECRO2013
  • Place of Presentation: レーベン、ベルギー
• [Presentation] SeeDB―A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: 国際シンポジウム Optogenetics2013
  • Place of Presentation: 慶應義塾大学三田キャンパス北館ホール（東京都）
• [Presentation] SeeDB―A simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction (2013)
  • Author(s): Meng-tsen Ke, Satoshi Fujimoto, Takeshi Imai
  • Organizer: 43rd Annual Meeting Society for Neuroscience
  • Place of Presentation: サンディエゴ、アメリカ
• [Remarks] 簡便で生体試料にやさしい組織透明化試薬「SeeDB」を開発 プレスリリース
  • URL: http://www.riken.jp/pr/press/2013/20130201_1/
• [Remarks] SeeDB resources
  • URL: https://sites.google.com/site/seedbresources/