Basic research on the ultrastructural framework of the route for diffuse modulatory transmission in CNS
Project/Area Number |
24650181
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Nerve anatomy/Neuropathology
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Research Institution | Iwate Medical University |
Principal Investigator |
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Research Collaborator |
ISHIDA Kinji 岩手医科大学, 医歯薬総合研究所, 技師長
HANASAKA Tomohito 岩手医科大学, 医歯薬総合研究所
MATSUURA Eri 岩手医科大学, 医歯薬総合研究所
OGASAWARA Katsutosi 岩手医科大学, 医歯薬総合研究所
NOZAKI Takayuki 岩手医科大学, 医歯薬総合研究所
ASADA Chikako 岩手医科大学, 医歯薬総合研究所
RICHARDSON William D University College London, Professor
ATTWELL David University College London, Professor
ANDERSON Patrick N University College London, Professor
LIEBERMAN Alexander R University College London, Emeritus Professor
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 細胞間隙 / 反射電子像 / 電子線トモグラフィー / 三次元再構築 / シナプス間隙 / Ranvier絞輪 / グリア細胞 / 拡散的神経情報伝達 / 電子顕微鏡 / 髄鞘 / ランヴィエ絞輪 / 反射電子 / 連続切片 / 三次元構築 / 中枢神経 |
Outline of Final Research Achievements |
Elucidation of the diffuse modulation system (DMS) is important to solve the role of neurons in the brainstem nuclei. To understand the 3D-ultrastructure (3DU) of the extra-cellular space (ECS), which support DMS transmission, we employed three high-technologies for electron microscopy, (i) rapid-freeze/substitution at ultra-low temperature (-269C) by liquid helium method (He-RF), (ii) double-axis electron beam tomography (DT), and (ii) back-scattered electron beam image analysis (BSE). We obtained following new findings: (1) ECS occupied about 3.7% of whole tissue in volume; (2) He-RF and DT demonstrateddetails of 3DU in the synaptic cleft ; (3) BSE made clear 3DU of the node: glial processes enwrapped only 25% area of node axon, and 75% open to ECS; (4) tear-drop like protrusions from the node with denatured organelle were found and exhibited phagocytic appearance by glia, suggesting the possibility of a new concept that axon-derived debris are processed by glia.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Altered magnetic resonance images of brain and social behaviors of hatchling, and expression of thyroid hormone receptor βmRNA in cerebellum of embryos after Methimazole administration2014
Author(s)
Gen Haba, Hidekazu Nishigori, Makoto Sasaki, Koujiro Tohyama, Kohsuke Kudo, Yutaka Matsumura, Toru Sugiyama, Keisuke Kagami, Yu Tezuka, Atsushi Sanbe, Hideo Nishigori
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Journal Title
Psychopharmacology
Volume: 231
Issue: 1
Pages: 221-230
DOI
Related Report
Peer Reviewed
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[Journal Article] Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships2013
Author(s)
Sawai T, Kamataki A, Uzuki M, Ishida K, Hanasaka T, Ochi K, Hashimoto T, Kubo T, Morikawa A, Ochi T, Tohyama K
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Journal Title
Microscopy (Oxf)
Volume: 62
Issue: 2
Pages: 317-320
DOI
Related Report
Peer Reviewed
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