| Project/Area Number |
24650181
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Research Collaborator |
ISHIDA Kinji 岩手医科大学, 医歯薬総合研究所, 技師長
HANASAKA Tomohito 岩手医科大学, 医歯薬総合研究所
MATSUURA Eri 岩手医科大学, 医歯薬総合研究所
OGASAWARA Katsutosi 岩手医科大学, 医歯薬総合研究所
NOZAKI Takayuki 岩手医科大学, 医歯薬総合研究所
ASADA Chikako 岩手医科大学, 医歯薬総合研究所
RICHARDSON William D University College London, Professor
ATTWELL David University College London, Professor
ANDERSON Patrick N University College London, Professor
LIEBERMAN Alexander R University College London, Emeritus Professor
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細胞間隙 / 反射電子像 / 電子線トモグラフィー / 三次元再構築 / シナプス間隙 / Ranvier絞輪 / グリア細胞 / 拡散的神経情報伝達 / 電子顕微鏡 / 髄鞘 / ランヴィエ絞輪 / 反射電子 / 連続切片 / 三次元構築 / 中枢神経 |
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| Outline of Final Research Achievements |
Elucidation of the diffuse modulation system (DMS) is important to solve the role of neurons in the brainstem nuclei. To understand the 3D-ultrastructure (3DU) of the extra-cellular space (ECS), which support DMS transmission, we employed three high-technologies for electron microscopy, (i) rapid-freeze/substitution at ultra-low temperature (-269C) by liquid helium method (He-RF), (ii) double-axis electron beam tomography (DT), and (ii) back-scattered electron beam image analysis (BSE).
We obtained following new findings: (1) ECS occupied about 3.7% of whole tissue in volume; (2) He-RF and DT demonstrateddetails of 3DU in the synaptic cleft ; (3) BSE made clear 3DU of the node: glial processes enwrapped only 25% area of node axon, and 75% open to ECS; (4) tear-drop like protrusions from the node with denatured organelle were found and exhibited phagocytic appearance by glia, suggesting the possibility of a new concept that axon-derived debris are processed by glia.
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