| Project/Area Number |
24650183
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Shinshu University (2013-2014)
National Institute for Physiological Sciences (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | シナプス / 神経実験形態学 |
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| Outline of Final Research Achievements |
To overcome disorders caused by deficit of synapse maturation, such as autism, we attempted to promote synapse maturation using DREADD system. For this, we introduced one type of DREADD, hM3Dq, into layer II/III pyramidal neurons in cerebral cortex in mouse brains, followed by administration of CNO to induce hM3Dq signal. We found that the synapse maturation was promoted in the hM3Dq expressing neurons judged from the morphology of dendritic spines. Furthermore, we found that this signal induced serine phosphorylation in the cytoplasmic region of Neurexin-1 that was known to be involved in synapse maturation, due to the activation of PKC.
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