| Project/Area Number |
24650188
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Ryota 順天堂大学, 医学部, 准教授 (40407284)
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| Co-Investigator(Renkei-kenkyūsha) |
HATTORI Nobutaka 順天堂大学, 医学部, 教授 (80218510)
URABE Takao 順天堂大学, 医学部, 教授 (60291663)
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| Project Period (FY) |
2012
|
| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | HSP27 / 脳梗塞 / 脳保護 / small heat shock protein / 一過性脳虚血再還流モデル |
|---|
| Research Abstract |
We examined the method of producing activated recombinant HSP27 for future clinical application of the new cerebral infarction treatment by HSP27 protein. Dephosphorylated recombinant HSP27 which made and purified from Escherichia coli had low brain protection activity. The recombinant HSP27 protein was phosphorylated. Phosphorylated recombinant HSP27 had brain protection. Infarct volume was reduced by 52% in stroke model mice when phosphorylated recombinant HSP27 was administered. We developed the method of mass-producing activated phosphoryled recombinant HSP27 in vitro.
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