Toward a development of the novel drug-screening system based on monitoring autophagy dynamics
| Project/Area Number |
24650189
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脳神経疾患 / 脳・神経 / トランスレーショナルリサーチ / 動物 / 神経科学 / 脳･神経 |
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| Outline of Final Research Achievements |
The autophagy-endolysosomal system is an evolutionally conserved degradation system that is tightly linked to a wide variety of physiological processes. Dysfunction of this system is associated with many neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). In this study, we sought to generate a novel analytical tool for the autophagy-endolysosomal system in vivo. First, we generated the expression construct encoding mKeimaRed_LC3B chimeric protein, and successfully detected the bimodal excitation spectrum of mKeimaRed_LC3B in HeLa cells under starved conditions. Then, we microinjected it into fertilized eggs from C57BL/6 mice, and transplanted them into the oviduct of pseudopregnant recipient mice. As a result, we have successfully established 2 lines of transgenic mouse expressing mKeima-Red_LC3B, particularly in heart, skeletal muscle and the central nervous system. These mice could become useful tools for development of the new drug-screening systems.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Six SQSTM1 mutations in a Chinese amyotrophic lateral sclerosis cohort.2015
Author(s)
Yang, Y., Tang, L., Zhang, N., Pan, L., Hadano, S., and Fan, D. -S.
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Journal Title
Amyotroph. Lateral Scler. Frontotempo. Degen.
Volume: in press
Issue: 5-6
Pages: 378-384
DOI
Related Report
Peer Reviewed
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[Journal Article] SQSTM1 mutations in Han Chinese populations with sporadic amyotrophic lateral sclerosis.2014
Author(s)
Chen, Y. P., Zheng, Z. Z., Chen, X. P., Huang, R., Yang, Y., Yuan, L. X., Pan, L., Hadano, S., and Shang, H. -F.
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Journal Title
Neurobiol. Aging
Volume: 35
Issue: 3
Pages: 726.e7-726.e9
DOI
Related Report
Peer Reviewed
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[Presentation] p62/SQSTM1 deficiency accelerates motor neuron degeneration in SOD1H46R transgenic mice.2014
Author(s)
Pan, L., Otomo, A., Koike, M., Uchiyama, Y., Aoki, M., Abe, K., Ishii, T., Yanagawa, T., Shang, H. -F., Yoshii, F., and Hadano, S.
Organizer
25th International Symposium on ALS/MND
Place of Presentation
Brussels, Belgium
Year and Date
2014-12-05 – 2014-12-07
Related Report
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[Presentation] Loss of p62/SQSTM1, an autophagy substrate, aggravates motor dysfunction in a SOD1H46R-expressing mouse ALS model.2014
Author(s)
Hadano, S., Pan, L., Otomo, A., Abe, K., Koike, M., Uchiyama, Y., Aoki, M., Ishii, T., Yanagawa, T., Shang, H. -F., and Yoshii, F.
Organizer
第37回日本神経科学大会
Place of Presentation
横浜
Year and Date
2014-09-11 – 2014-09-13
Related Report
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[Presentation] Loss of p62/SQSTM1 exacerbates motor dysfunction in a mutant SOD1-expressing mouse ALS model.2013
Author(s)
Pan, L., Otomo, A., Abe, K., Ogawa, H., Chiba, T., Koike, M., Uchiyama, Y., Aoki, M., Yoshii, F., Ishii, T., Yanagawa, T., and Hadano, S.
Organizer
24th International Symposium on ALS/MND
Place of Presentation
Milan, Italy
Related Report
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[Presentation] SQSTM1 mutations in sporadic Chinese patients with amyotrophic lateral sclerosis.2013
Author(s)
Chen, Y., Chen, X., Huang, R., Zheng, Z., Wei, Q., Guo, X., Pan, L., Hadano, S., and Shang, H.
Organizer
24th International Symposium on ALS/MND
Place of Presentation
Milan, Italy
Related Report
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