Research Project
Grant-in-Aid for Challenging Exploratory Research
The autophagy-endolysosomal system is an evolutionally conserved degradation system that is tightly linked to a wide variety of physiological processes. Dysfunction of this system is associated with many neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). In this study, we sought to generate a novel analytical tool for the autophagy-endolysosomal system in vivo. First, we generated the expression construct encoding mKeimaRed_LC3B chimeric protein, and successfully detected the bimodal excitation spectrum of mKeimaRed_LC3B in HeLa cells under starved conditions. Then, we microinjected it into fertilized eggs from C57BL/6 mice, and transplanted them into the oviduct of pseudopregnant recipient mice. As a result, we have successfully established 2 lines of transgenic mouse expressing mKeima-Red_LC3B, particularly in heart, skeletal muscle and the central nervous system. These mice could become useful tools for development of the new drug-screening systems.
All 2015 2014 2013 2012 Other
All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (25 results) (of which Invited: 3 results) Book (1 results)
Amyotroph. Lateral Scler. Frontotempo. Degen.
Volume: in press Issue: 5-6 Pages: 378-384
10.3109/21678421.2015.1009466
Neurobiol. Aging
Volume: 35 Issue: 3 Pages: 726.e7-726.e9
10.1016/j.neurobiolaging.2013.09.008
PLoS One
Volume: 7巻 Issue: 3 Pages: e33409-e33409
10.1371/journal.pone.0033409
Free Radical Biol. Med.
Volume: 53 Issue: 11 Pages: 2028-2042
10.1016/j.freeradbiomed.2012.09.010
Neurol. Res. Int
Volume: 2012 Pages: 498428-498428
10.1155/2012/498428