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A new multitasking gene specifically developed for the analysis of brain functions

Research Project

Project/Area Number 24650195
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNiigata University

Principal Investigator

SAKIMURA Kenji  新潟大学, 脳研究所, 教授 (40162325)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords遺伝子 / ノックアウトマウス / Cre/loxP / Flp/frt / 脳機能解析
Research Abstract

In the analysis of brain functions using conditional knockout mice, the ability of Cre driver mice is important and determines the quality of research. The purpose of this study is to produce a new multitasking cassette which can express a specific gene in a cell-specific manner without affecting the endogenous gene expression. When a multitasking cassette is inserted into a specific driver gene, its driver gene is knocked out. Next, the stop sequences are removed, Cre can be activated under the corresponding promoter with the endogenous gene expression kept intact. Also, the corresponding gene expression can be rescued by the Flpase. After checking the function of this cassette in cultured cells, we next produced a knockin mouse with the cassette integrated in the Abat gene. The generated Abat-knockin mice and their Cre activity is being checked using the lacZ reporter mice. This system can contribute in generating driver mice with only one line, for the use of many purposes.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2013 2012 Other

All Presentation (16 results) Remarks (1 results)

  • [Presentation] 発達期小脳における顆粒細胞選択的かつ調節可能な組換え酵素Cre発現マウスの作製2013

    • Author(s)
      Peng Fei
    • Organizer
      分子生物学会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットES細胞から遺伝子改変動物作製の効率化2013

    • Author(s)
      中務胞
    • Organizer
      凍結胚を用いたキメラ胚作出法分子生物学会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Low affinity kainate receptors are involved with motor functions in mouse2013

    • Author(s)
      I. WATANABE
    • Organizer
      Neuroscience2013
    • Place of Presentation
      アメリカ・サンディエゴ
    • Year and Date
      2013-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Nna1 is related to motor learning, anxiety-related behaviors and dynamics of glutamate receptor subunits2013

    • Author(s)
      L. ZHOU
    • Organizer
      Neuroscience2013
    • Place of Presentation
      アメリカ・サンディエゴ
    • Year and Date
      2013-11-11
    • Related Report
      2013 Final Research Report
  • [Presentation] カイニン酸受容体サブユニットGluK2ノックアウトマウスでは、運動障害がみられるKainate receptor GluK2 subunit-deficient mouse shows motor dis coordination2013

    • Author(s)
      渡辺和泉
    • Organizer
      第36回日本神経科学大会/第56回日本神経化学会NEURO2013
    • Place of Presentation
      京都府京都市
    • Year and Date
      2013-06-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantitative analysis of kainite subunits and delta subunits of glutamate receptor in the mouse brain2013

    • Author(s)
      K.Sakimura
    • Organizer
      ISN24th Biennial Meeting
    • Place of Presentation
      メキシコ・カンクン
    • Year and Date
      2013-04-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Systematic generation of conditional gene targeting mice developed using C57BL/6 ES cell line RENKA for analysis of brain functions2012

    • Author(s)
      M.Abe
    • Organizer
      Neuroscience2012
    • Place of Presentation
      New Orleans(USA)
    • Year and Date
      2012-10-14
    • Related Report
      2013 Final Research Report
  • [Presentation] C57BL/6系統ES細胞RENKAを用いた系統的遺伝子改変マウス作製法の効率2012

    • Author(s)
      夏目里恵
    • Organizer
      第59回日本実験動物学会総会
    • Place of Presentation
      大分県別府市
    • Year and Date
      2012-05-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantitative analysis of kainite subunits and delta subunits of glutamate receptor in the mouse brain

    • Author(s)
      K.Sakimura,et al.
    • Organizer
      ISN 24th Biennial Meeting
    • Place of Presentation
      メキシコ・カンクン
    • Related Report
      2013 Annual Research Report
  • [Presentation] カイニン酸受容体サブユニットGluK2 ノックアウトマウスでは、運動障害がみられる  Kainate receptor GluK2 subunit-deficient mouse shows motor dis coordination

    • Author(s)
      渡辺和泉 他5名
    • Organizer
      第36回日本神経科学大会/第56回日本神経化学会NEURO2013
    • Place of Presentation
      京都府京都市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Nna1 is related to motor learning, anxiety-related behaviors and dynamics of glutamate receptor subunits

    • Author(s)
      L. ZHOU, et al.
    • Organizer
      Neuroscience2013
    • Place of Presentation
      アメリカ・サンディエゴ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Low affinity kainate receptors are involved with motor functions in mouse

    • Author(s)
      I. WATANABE , et al.
    • Organizer
      Neuroscience2013
    • Place of Presentation
      アメリカ・サンディエゴ
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラットES細胞から遺伝子改変動物作製の効率化:凍結胚を用いたキメラ胚作出法

    • Author(s)
      中務胞 他4名
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      兵庫県神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 発達期小脳における顆粒細胞選択的かつ調節可能な組換え酵素Cre発現マウスの作製

    • Author(s)
      Peng Fei 他4名
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      兵庫県神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] C57BL/6系統ES細胞RENKAを用いた系統的遺伝子改変マウス作製法の効率

    • Author(s)
      夏目里恵
    • Organizer
      第59回日本実験動物学会総会
    • Place of Presentation
      大分県別府市
    • Related Report
      2012 Research-status Report
  • [Presentation] Systematic generation of conditional gene targeting mice developed using C57BL/6 ES cell line RENKA for analysis of brain functions

    • Author(s)
      M.Abe
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      New Orleans(USA)
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.bri.niigata-u.ac.jp/~cellular/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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