Phenotype analysis on conditional knockout mice defective of myosin VI
Project/Area Number |
24650196
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Kanazawa University |
Principal Investigator |
YONEDA Yukio 金沢大学, 薬学系, 教授 (50094454)
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | ミオシンVI / PTSD / 神経幹細胞 / 胚性腫瘍細胞 / 神経細胞 / アストログリア細胞 / 細胞増殖能 / 細胞分化能 / ミオシンファミリー / 多能性P19細胞 / ミオシン / フロックスマウス / Creリコンビナーゼ |
Outline of Final Research Achievements |
Severe fatal stress induced long-lasting bidirectional behavioral abnormalities relevant to posttraumatic stress disorder in adult mice, along with upregulation of the transcript and protein for myosin VI (Myo6) only in the hippocampus known to be enriched of proliferating neural progenitor cells. In embryonal carcinoma pluripotent P19 cells endowed to proliferate and differentiate into neuronal and astroglial lineages, stable overexpression of Myo6 attenuated the size and the activities of MTT reduction and BrdU incorporation in neurospheres clustered of proliferating cells. In these stable transfectants, no significant change was seen in the activity to commit and differentiate into neuronal and astroglial lineages. We succeeded in the generation of mice carrying a conditional Myo6 allele flanked by loxP sites, which are useful for generating mice detractive of Myo6 from a particular cell type for future investigation of the role of Myo6 in a variety of plasma cells.
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Report
(4 results)
Research Products
(73 results)
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[Journal Article] Myosin VI reduces proliferation, but not differentiation, in pluripotent P19 cells2013
Author(s)
Takeshi Takarada, Miki Kou, Noritaka Nakamichi, Masato Ogura, Yuma Ito, Ryo Fukumori, Hiroshi Kokubo, Gabriela B. Acosta, Eiichi Hinoi and Yukio Yoneda
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Journal Title
PLoS ONE
Volume: 8
Issue: 5
Pages: e63947-e63947
DOI
Related Report
Peer Reviewed
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[Journal Article] An analysis of skeletal development in osteoblast- and chondrocyte-specific Runx2 knockout mice2013
Author(s)
Takeshi Takarada, Eiichi Hinoi, Ryota Nakazato, Hiroki Ochi, Cheng Xu, Azusa Tsuchikane, Shu Takeda, Gerard Karsenty, Takaya Abe, Hiroshi Kiyonari, and Yukio Yoneda
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Journal Title
J. Bone Miner. Res
Volume: 未定
Issue: 10
Pages: 2064-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Positive regulation of osteoclastic differentiation by growth differentiation factor-15 up-regulated in osteocytic cells under hypoxia2012
Author(s)
Eiichi Hinoi, Hiroki Ochi, Takeshi Takarada, Eri Nakatani, Takashi Iezaki, Hiroko Nakajima, Hiroyuki Fujita, Yoshifumi Takahata, Shinya Hidano, Takashi Kobayashi, Shu Takeda, Yukio Yoneda
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Journal Title
J Bone Miner Res
Volume: 4
Issue: 4
Pages: 938-949
DOI
Related Report
Peer Reviewed
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