| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Severe fatal stress induced long-lasting bidirectional behavioral abnormalities relevant to posttraumatic stress disorder in adult mice, along with upregulation of the transcript and protein for myosin VI (Myo6) only in the hippocampus known to be enriched of proliferating neural progenitor cells. In embryonal carcinoma pluripotent P19 cells endowed to proliferate and differentiate into neuronal and astroglial lineages, stable overexpression of Myo6 attenuated the size and the activities of MTT reduction and BrdU incorporation in neurospheres clustered of proliferating cells. In these stable transfectants, no significant change was seen in the activity to commit and differentiate into neuronal and astroglial lineages. We succeeded in the generation of mice carrying a conditional Myo6 allele flanked by loxP sites, which are useful for generating mice detractive of Myo6 from a particular cell type for future investigation of the role of Myo6 in a variety of plasma cells.
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