| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In a carefully maintained conventional serum-free culture medium, primary cultured rat hippocampal neurons could survive over 5~6 months (M), and significant portion of those neurons could remain nearly one year in the regular culture dishes. In this long term in vitro culture system, neurons initially proliferate, mature, then start to show evidence of senescence after a period of synaptic and/or neuronal elimination, and subsequently leading to the stage of neuronal loss or death, which could be arbitrarily divided into five consecutive phases; the growing phase I (~1M), the maturation phase II (1~2M), the early aging phase III (3~6M), the late aging (or middle senescence) phase IV (7~9M), and the late senescence phase V (10M and over). Even in the senescent phases, some neuronal dendrites retained spines containing postsynaptic components. We propose that this system could be a useful model for the study of non-replicative post-mitotic neuronal cell senescence.
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