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Functional expression of mammalian ion channels and analysis of the molecular mechanisms

Research Project

Project/Area Number 24650203
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionInstitute for Molecular Science

Principal Investigator

FURUTANI Yuji  分子科学研究所, 生命・錯体分子科学研究領域, 准教授 (80432285)

Co-Investigator(Kenkyū-buntansha) NAKAJO Koichi  生理学研究所, 分子生理研究系, 助教 (80390699)
Co-Investigator(Renkei-kenkyūsha) TSUKAMOTO Hisao  分子科学研究所, 生命・錯体分子科学研究領域, 助教 (90579814)
KIMURA Tetsunari  理化学研究所, 播磨事業所, 研究員 (70506906)
KUBO Yoshihiro  生理学研究所, 分子生理研究系, 教授 (80211887)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords赤外分光法 / イオンチャネル / 生物物理学 / 分子科学 / 振動分光法
Research Abstract

Ion channel proteins work for selective permeation of ions in response to various stimuli and regulate electrical signals in living organisms. To understand the molecular mechanisms of these channels, the precise structural information with atomic resolution is required. In this study, mammalian ion channel proteins, which are important targets in medical and pharmaceutical sciences, have been studied by attenuated total reflection (ATR) FT-IR spectroscopy which gives us precise molecular information. After screening of several ion channel proteins, we have succeeded to resolve amide I bands of selectivity filter of KCNK1 channel, which is involved in regulation of resting membrane potential of cell, by using ATR FT-IR spectroscopy.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (27 results)

All 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (20 results) (of which Invited: 14 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Large spectral change due to amide modes of a β-sheet upon the formation of an early photointermediate of middle rhodopsin2013

    • Author(s)
      Furutani, Y., Okitsu, T., Reissig, L., Mizuno, M., Homma, M., Wada, A., Mizutani, Y., Sudo, Y.
    • Journal Title

      J. Phys. Chem. B

      Volume: 117 Issue: 13 Pages: 3449-3458

    • DOI

      10.1021/jp308765t

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ATR-FTIR Spectroscopy Revealed the Different Vibrational Modes of the Selectivity Filter Interacting with K+ and Na+ in the Open and Collapsed Conformations of the KcsA Potassium Channel2012

    • Author(s)
      Furutani, Y., Shimizu, H., Asai, Y., Fukuda, T., Oiki, S. and Kandori, H
    • Journal Title

      J. Phys. Chem. Lett

      Volume: 3 Issue: 24 Pages: 3806-3810

    • DOI

      10.1021/jz301721f

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distortion of the Amide-I and -II Bands of an α-helical Membrane Protein, pharaonis Halorhodopsin, Depends on Thickness of Gold Films Utilized for Surface-Enhanced Infrared Absorption Spectroscopy2012

    • Author(s)
      H. Guo, T. Kimura, and Y. Furutani
    • Journal Title

      Chem. Phys.

      Volume: - Pages: 8-16

    • DOI

      10.1016/j.chemphys.2012.11.011

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamics of Dangling Bonds of Water Molecules in pharaonis Halorhodopsin during Chloride Ion Transportation2012

    • Author(s)
      Furutani, Y., Fujiwara, K., Kimura, T., Kikukawa, T., Demura, M., Kandori, H
    • Journal Title

      J. Phys. Chem. Lett

      Volume: 3 Issue: 20 Pages: 2964-2969

    • DOI

      10.1021/jz301287n

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Water Molecules and Ions in Membrane Proteins Studied by FTIR th来のカリウムチャネルの発現コンストラクSpectroscopy2013

    • Author(s)
      Yuji Furutani
    • Organizer
      the 2012 OCARINA Annual International Meeting
    • Place of Presentation
      Osaka City Univ. Media Center, Osaka, Japan
    • Year and Date
      2013-05-04
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 哺乳動物カリウムチャネルの赤外分光解析2013

    • Author(s)
      塚本寿夫、中條浩一、久保義弘、古谷祐詞
    • Organizer
      平成24年度生物物理学会中部支部講演会
    • Place of Presentation
      名古屋(名古屋大学)(口頭)
    • Year and Date
      2013-02-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Stimulus-Induced Difference FTIR Spectroscopy on Ion-Channel and Ion-Pump Proteins2013

    • Author(s)
      Yuji Furutani
    • Organizer
      Sixth Korea-Japan Seminars on Biomolecular Sciences : Experiments and Simulations
    • Place of Presentation
      Okazaki Conference Center, Okazaki, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 哺乳類two-pore型カリウムチャネルTWIK-1の全反射赤外分光解析2013

    • Author(s)
      塚本寿夫、中條浩一、久保義弘、古谷祐詞
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都(京都国際会議場)
    • Related Report
      2013 Final Research Report
  • [Presentation] Water Molecules and Ions in Membrane Proteins Studied by FTIR Spectroscopy2013

    • Author(s)
      Yuji Furutani
    • Organizer
      Annual Meeting on Photochemistry 2013
    • Place of Presentation
      Ehime University, Matsuyama, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Water Molecules and Ions in Membrane Proteins Studied by Stimulus-induced Difference FTIR Spectroscopy2013

    • Author(s)
      Yuji Furutani
    • Organizer
      Seventh International Conference on Advanced Vibrational Spectroscopy
    • Place of Presentation
      Kobe Convention Center, Kobe, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Stimulus-Induced Difference FTIR Spectroscopy Reveals Structural Dynamics thしていることと一致している。さらに、現在of Membrane Proteins2013

    • Author(s)
      Yuji Furutani
    • Organizer
      Symposium on Molecular Science Hierarchical Structure from Quantum to Functions of Biological Systems
    • Place of Presentation
      Hotel Kitano Plaza Rokkoso, Kobe, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 哺乳類two-pore型カリウムチャネルTWIK-1の全反射赤外分光解析2013

    • Author(s)
      塚本寿夫、中條浩一、久保義弘、古谷祐詞
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都国際会館(京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ion-protein Interactions Studied by ATR-FTIR Spectroscopy2012

    • Author(s)
      Yuji Furutani
    • Organizer
      17^<th> Malaysian Chemical Congress (17MCC)
    • Place of Presentation
      Putra World Trade Centre, Kuala Lumpur, Malaysia
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 生物物理学的解析を目指した、哺乳培養細胞を用いた哺乳類カリウムイオンチャネルの大量発現の試み2012

    • Author(s)
      塚本寿夫、中條浩一、久保義弘、古谷祐詞
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋(名古屋大学)(ポスター)
    • Related Report
      2013 Final Research Report
  • [Presentation] Ion-Protein Interactions Studied by ATR-FTIR Spectroscopy2012

    • Author(s)
      Yuji Furutani
    • Organizer
      The 16th East Asian Workshop on Chemical Dynamics
    • Place of Presentation
      National Tsing Hua University, Hsinchu, Taiwan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 生物物理学的解析を目指した、哺乳培養細胞を用いた哺乳類カリウムイオンチャネルの大量発現の試み2012

    • Author(s)
      塚本寿夫、中條浩一、久保義弘、古谷祐詞
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(愛知県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Stimulus-Induced Difference FTIR Spectroscopy Reveals Structural Dynamics of Membrane Proteins

    • Author(s)
      Yuji Furutani
    • Organizer
      15th Japan-Korea Symposium on Molecular Science Hierarchical Structure from Quantum to Functions of Biological Systems
    • Place of Presentation
      ホテル北野プラザ六甲荘 (神戸)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Water Molecules and Ions in Membrane Proteins Studied by Stimulus-induced Difference FTIR Spectroscopy

    • Author(s)
      Yuji Furutani
    • Organizer
      Seventh International Conference on Advanced Vibrational Spectroscopy
    • Place of Presentation
      神戸コンベンションセンター(神戸)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Water Molecules and Ions in Membrane Proteins Studied by FTIR Spectroscopy

    • Author(s)
      Yuji Furutani
    • Organizer
      Annual Meeting on Photochemistry 2013
    • Place of Presentation
      愛媛大学(愛媛県松山市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Stimulus-Induced Difference FTIR Spectroscopy on Ion-Channel and Ion-Pump Proteins

    • Author(s)
      Yuji Furutani
    • Organizer
      Sixth Korea-Japan Seminars on Biomolecular Sciences: Experiments and Simulations
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 哺乳動物カリウムチャネルの赤外分光解析

    • Author(s)
      塚本寿夫、中條浩一、久保義弘、古谷祐詞
    • Organizer
      平成24年度 生物物理学会中部支部 講演会
    • Place of Presentation
      名古屋大学(愛知県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Water Molecules and Ions in Membrane Proteins Studied by FTIR Spectroscopy

    • Author(s)
      Yuji Furutani
    • Organizer
      The 5th International Conference as the 2012 OCARINA Annual International Meeting
    • Place of Presentation
      Osaka City Univ. Media Center (Osaka)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Ion-protein Interactions Studied by ATR-FTIR Spectroscopy

    • Author(s)
      Yuji Furutani
    • Organizer
      17th Malaysian Chemical Congress (17MCC) 2012
    • Place of Presentation
      Putra World Trade Centre (Malaysia)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Ion-Protein Interactions Studied by ATR-FTIR Spectroscopy

    • Author(s)
      Yuji Furutani
    • Organizer
      The 16th East Asian Workshop on Chemical Dynamics
    • Place of Presentation
      National Tsing Hua University (Taiwan)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 「微生物型ロドプシンの光誘起イオン輸送メカニズムの解明」,オプトジェネティクス-光光学と遺伝学による行動制御技術の最前線-2013

    • Author(s)
      古谷祐詞
    • Publisher
      NTS
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://groups.ims.ac.jp/organization/furutani_g/

    • Related Report
      2013 Final Research Report
  • [Remarks] 分子科学研究所生命・錯体分子科学研究領域 古谷グループ

    • URL

      http://groups.ims.ac.jp/organization/furutani_g/index.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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