Development of a novel in vivo imaging system and its application to place cells
| Project/Area Number |
24650209
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neurophysiology and muscle physiology
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KUNITAKE Takato 宮崎大学, 医学部, 助教 (20234461)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | in vivo イメージング / 自由行動 / 海馬 / 神経活動 |
|---|
| Research Abstract |
In the present work, we tried to develop a novel in vivo imaging system, which can be equipped with a freely moving animal for a long period, to understand higher brain functions.
We first made green fluorescence protein (GFP)-expressing lentivirus vector, and then injected it to mouse hippocampus CA1 region. A few weeks later, strong GFP signals were observed in the region of the acute slice under a fluorescence microscope. In addition, the lentivirus infection had no influence on electrophysiological properties, including long-term potentiation (LTP) expression, of GFP-expressed neurons. Next, we tried to observed fluorescence images of the similar slice via gradient index (GRIN) lens, however, the images were faint. GRIN lens is thought to play a key role in in vivo imaging system because it transfer the images from the inside of the brain to the outside. In the future, therefore, it is necessary to improve the system by increasing signal-to-noise ratio.
|
Report
(3 results)
Research Products
(5 results)
