Development of a next-generation photo-stimulation device

• Project/Area Number: 24650214
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fusional basic brain science
• Research Institution: Saitama University
• Principal Investigator: NAKAI Junichi 埼玉大学, 脳科学融合研究センター, 教授 (80237198)
• Co-Investigator(Renkei-kenkyūsha): ANDO Keiko 埼玉大学, 脳科学融合研究センター, 特任 准教授 (40221741)
• Research Collaborator: OHKURA Masamichi 埼玉大学, 脳科学融合研究センター, 准教授 (70369172)
• Project Period (FY): 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 光遺伝学 / オプトジェネティクス / 第二高調波 / 極超短赤外線パルスレーザー / KTN 結晶 / 第二高調波発生 / 極長短赤外線パルスレーザー / KTN結晶

Research Abstract

In this study we developed a microscope equipment with which photosensitive molecules can be stimulated. In the equipment a pair of potassium tantalate niobate (KTN) crystals were used as a tunable lens, an electro-optic device that changes its refractive index by voltage. By changing the voltage which applied to the tunable KTN lens focus changed within ~23 microseconds. The speed of focus change was about 500~1000 times faster than that of the conventional piezo focuser. This technique can be applicable not only to scientific researches including neuroscience but also industrial field such as laser processing.

Research Products

• [Journal Article] Real-time visualization of neuronal activity during perception (2013)
  • Author(s): Muto A, Ohkura M, Abe G, Nakai J, Kawakami K
  • Journal Title: Current Biology Volume: 23 Issue: 4 Pages: 307-311
  • DOI: 10.1016/j.cub.2012.12.040
  • Peer Reviewed
• [Journal Article] Astrocytic Ca2+ signals are required for the functional integrity of tripartite synapses (2013)
  • Author(s): Tanaka M
  • Journal Title: Molecular Brain Volume: 6 Issue: 1 Pages: 6-6
  • DOI: 10.1186/1756-6606-6-6
  • Peer Reviewed
• [Journal Article] Cilia at the Node of Mouse Embryos Sense Fluid Flow for Left-Right Determination via Pkd2 (2012)
  • Author(s): Yoshiba S, Shiratori H, Kuo IY, Kawasumi A, Shinohara K, Nonaka S, Asai Y, Sasaki G, Belo JA, Sasaki H, Nakai J, Dworniczak B, Ehrlich BE, Pennekamp P, Hamada H
  • Journal Title: Science Volume: 338(6104):226-3 Issue: 6104 Pages: 226-231
  • DOI: 10.1126/science.1222538
  • Peer Reviewed
• [Journal Article] Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system (2012)
  • Author(s): K. Tanaka, K. Matsui, T. Sasaki. H. Sano, S. Sugio, K. Fan, K. Ikenaka, R. Hen, J. Nakai, Y. Yanagawa, H. Hasuwa, K. Deisseroth, A. Yamanaka
  • Journal Title: Cell Reports Volume: 2 Issue: 2 Pages: 397-406
  • DOI: 10.1016/j.celrep.2012.06.011
  • Peer Reviewed
• [Journal Article] An improved genetically encoded red fluorescent Ca2+ indicator for detecting opticallyevoked action potentials (2012)
  • Author(s): M. Ohkura, T. Sasaki. C. Kobayashi, Y. Ikegaya, J. Nakai
  • Journal Title: PLoS ONE Volume: 7 Issue: 7 Pages: 1-7
  • DOI: 10.1371/journal.pone.0039933
  • Peer Reviewed
• [Journal Article] Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals (2012)
  • Author(s): M. Ohkura, T. Sasaki. J. Sadakari, K. Gengyo-Ando, Y. Kagawa-Nagamura, C. Kobayashi, Y. Ikegaya, J. Nakai
  • Journal Title: PLoS ONE Volume: 7 Issue: 12 Pages: 1-10
  • DOI: 10.1371/journal.pone.0051286
  • Peer Reviewed
• [Presentation] Development and application of G-CaMP-type genetically encoded Ca2+ indicators (2012)
  • Author(s): Ohkura M, Nakai J
  • Organizer: 2012 Fall Janelia Conference "Fluorescent Proteins and Biological Sensors III
  • Place of Presentation: Ashburn USA
• [Presentation] Dynamic neuromuscular regulation in freely crawling C. elegans: multicellular Ca2+ imaging using GCaMPs (2012)
  • Author(s): Gengyo-Ando K, Usami A, Kagawa-Nagamura Y,Ohkura M, Ikegaya Y, Matsuki N, Nakai J
  • Organizer: 第35回神経科学学会年会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Development and application of G-CaMP-type genetically encoded Ca2+ indicators. (2012)
  • Author(s): Ohkura M, Nakai J
  • Organizer: 2012 Fall Janelia Conference “Fluorescent Proteins and Biological Sensors III.”
  • Place of Presentation: Ashburn USA
  • Invited
• [Presentation] Dynamic neuromuscular regulation in freely crawling C. elegans: multicellular Ca2+ imaging using G-CaMPs. (2012)
  • Author(s): Gengyo-Ando K, Usami A, Kagawa-Nagamura Y, Ohkura M, Ikegaya Y, Matsuki N, Nakai J
  • Organizer: 第35回神経科学学会年会
  • Place of Presentation: 名古屋国際会議場
  • Invited
• [Remarks] 埼玉大学脳科学融合研究センター
  • URL: http://subsi.saitama-u.ac.jp/
• [Remarks] 報道関連情報(1)Kawabata I, Kashiwagi I, Obashi K, Ohkura M, Nakai J, Wynshaw-Boris A, Yanagawa Y and Okabe S:Moving synapses on developing neurons. Nature 姉妹誌注目のハイライト、2012 年3月7日.
• [Remarks] (2)日経産業新聞: Muto A, Ohkura M et al, CurrentBiology 23, 1-5, 2013の論文に関 る記事が掲載された。内容:脳活動生きたまま観察.報道年月日:H25.2.13
• [Remarks] Muto A, Ohkura M et al, Current Biology 23,1-5, 2013 の論文が国内外のインターネット サイト(以下)に大きく取り上げられた。
• [Remarks] (1)ロイター
  • URL: http://www.reuters.com/news/video-videoChannel=6
• [Remarks] (2)サイエンスマガジン
  • URL: http://news.sciencemag.org/sciencenow/2013/01/whats-your-fish-thinking.html
• [Remarks] (3)サイエンティフィックアメリカン
  • URL: https://www.scientificamerican.com/article.cfm?id=scientists-watch-a-fish-think
• [Remarks] (4)ナショナルジオグラフィック
  • URL: http://phenomena.nationalgeographic.com/2013/01/31/fish-watches-food-scientists-watch-fishs-thoughts/
• [Remarks] (5)ワイアード
  • URL: http://www.wired.com/wiredscience/2013/02/fish-neuron-video/
• [Remarks] (6)ニューサイエンティスト
  • URL: http://www.newscientist.com/article/dn23123-fluorescent-protein-lets-us-read-a-fishs-thoughts.html
• [Remarks] (7)ネイキッドサイエンティスト
  • URL: http://www.thenakedscientists.com/HTML/content/news-archive/news/1000067/
• [Remarks] (8)ロサンゼルスタイムズ
  • URL: http://www.latimes.com/news/science/sciencenow/la-sci-sn-fish-thought-brain-swim-zebrafish-20130131,0,5543599.story
• [Remarks] (9)マイナビニュース
  • URL: http://news.mynavi.jp/news/2013/02/01/182/
• [Remarks] (10)カラパイア
  • URL: http://karapaia.livedoor.biz/archives/52116852.html
• [Remarks] (11)GIZMOND世界初!脳の電気的な動きをリアルタイムで可視化
  • URL: http://www.gizmodo.jp/2013/02/post_11642.html
• [Remarks] (12)コモンポスト
  • URL: http://commonpost.info/?tag=%E3%82%BC%E3%83%96%E3%83%A9%E3%83%95%E3%82%A3%E3%83%83%E3%82%B7%E3%83%A5
• [Remarks] (13)アメーバニュース
  • URL: http://news.ameba.jp/20130214-547/
• [Remarks] (3)大倉正道:蛍光分子センサーの開発と神経回路研究への応用.日本経団連埼玉埼経協ニュース371,18-18,(2012).
• [Remarks] 埼玉大学脳科学融合研究センター
  • URL: http://subsi.saitama-u.ac.jp/