| Project/Area Number |
24650216
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Fusional basic brain science
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| Research Institution | Kyoto University |
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Principal Investigator |
WANG Dan Ohtan 京都大学, 物質-細胞統合システム拠点, 助教 (50615482)
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| Co-Investigator(Renkei-kenkyūsha) |
OKAMOTO Akimitsu (60314233)
SUGI Takuma (70571305)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | イメージング / RNA / 空間分布 / 転写 / 小脳 / RNA ダイナミズム / in vivo / イメージング技術 / プローブテクノロジー / 記憶 |
|---|
| Research Abstract |
De novo transcription is required for long-term plasticity and lasting memory formation. We are developing live-cell RNA imaging techniques to visualize RNA dynamics in a living and functional neuronal circuit where memory information is stored. To do so, we have delivered light-on probes into the living mouse cerebella and imaged RNA molecules at single cell level. Curiously, we have observed spatial organization of RNA molecules that are concentrated at specific intranuclear foci. We are currently optimizing the system to apply the imaging method to memory studies.
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