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Detection of circadian rhythms from peripheral blood samples in the diagnosis of diseases of elderly people

Research Project

Project/Area Number 24650217
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Fusional basic brain science
Research InstitutionKyoto University

Principal Investigator

OKAMURA Hitoshi  京都大学, 大学院・薬学研究科, 教授 (60158813)

Project Period (FY) 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords遺伝子 / 循環器・高血圧 / トランスレーションリサーチ / バイオテクノロジー
Research Abstract

We established a sensitive method for detecting clock genes at single cell levels in cells derived from human lymphocyte. We collected cells by cell sorter, and RNA isolation and reverse transcription to make cDNA at single step. We have established a method to detect all clock genes at the level of single cells in the peripheral blood.

Report

(2 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (10 results)

All 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (2 results) (of which Invited: 1 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Development of diurnal micturition pattern in mice after weaning.2013

    • Author(s)
      Negoro H
    • Journal Title

      Journal of Urology

      Volume: 189 Issue: 2 Pages: 740-746

    • DOI

      10.1016/j.juro.2012.07.140

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of urinary bladder ConnexTn43 ana tne circadian clock in coordination of diurnal micturition rhythm2012

    • Author(s)
      Negoro H
    • Journal Title

      Nature Communications

      Volume: 3 Issue: 1 Pages: 809-809

    • DOI

      10.1038/ncomms1812

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rhythmic nucleotide synthesis in tne liver: Temporal segregation of metabolites2012

    • Author(s)
      Fustin J-M
    • Journal Title

      Cell Reports

      Volume: 1 Issue: 4 Pages: 341-349

    • DOI

      10.1016/j.celrep.2012.03.001

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 体内時計概論2012

    • Author(s)
      山田 裕之、岡村 均
    • Journal Title

      Bio Clinica

      Volume: 27 Pages: 532-537

    • Related Report
      2012 Final Research Report
  • [Presentation] 生体リズム異常と疾病:臓器の時計とその異常2012

    • Author(s)
      岡村 均
    • Organizer
      第21回日本小児泌尿器科学術総会
    • Place of Presentation
      岡山コンベンショんセンター(岡山市)
    • Year and Date
      2012-07-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 生体リズム異常と疾病:臓器の時計とその異常2012

    • Author(s)
      岡村 均
    • Organizer
      第21回日本小児泌尿器科学術総会
    • Place of Presentation
      岡山コンベンションセンター(岡山市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] 臓器円環による生体恒常性のダイナミクス~神経・免疫・循環・内分泌系の連関による維持,ライフステージに応じた変容と破綻2013

    • Author(s)
      永井良三
    • Total Pages
      222
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] 臓器円環による生体恒常性のダイナミクス~神経・免疫・循環・内分泌系の連関による維持,ライフステージに応じた変容と破綻 (実験医学増刊 Vol. 31-5)2013

    • Author(s)
      永井 良三
    • Total Pages
      222
    • Publisher
      羊土社
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.pharm.kyoto-u.ac.jp/system-biology/

    • Related Report
      2012 Final Research Report
  • [Remarks] 京都大学薬学研究科システムバイオロジー分野

    • URL

      http://www.pharm.kyoto-u.ac.jp/system-biology/

    • Related Report
      2012 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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