Detection of circadian rhythms from peripheral blood samples in the diagnosis of diseases of elderly people
| Project/Area Number |
24650217
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fusional basic brain science
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| Research Institution | Kyoto University |
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Principal Investigator |
OKAMURA Hitoshi 京都大学, 大学院・薬学研究科, 教授 (60158813)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 遺伝子 / 循環器・高血圧 / トランスレーションリサーチ / バイオテクノロジー |
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| Research Abstract |
We established a sensitive method for detecting clock genes at single cell levels in cells derived from human lymphocyte. We collected cells by cell sorter, and RNA isolation and reverse transcription to make cDNA at single step. We have established a method to detect all clock genes at the level of single cells in the peripheral blood.
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Report
(2 results)
Research Products
(10 results)
