MicroRNA microarray analysis of dopaminergic neurons from FFPE brain sections of patients with Parkinson's disease

• Project/Area Number: 24650222
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fusional brain recording science
• Research Institution: Teikyo University
• Principal Investigator: KIKUCHI-UTSUMI Kazue 帝京大学, 医学部, 助教 (90271759)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: レーザーマイクロダイセクション / ホルマリン固定パラフィン包埋標本 / microRNA / ドパミン神経細胞 / パーキンソン病 / レーザーマイクロダイセク ション / ホルマリン固定パラフィン 包埋切片標本 / FFPE / マイクロRNA / マイクロアレイ

Outline of Final Research Achievements

The purpose of this study is to establish miRNA microarray analysis of the dopaminergic neurons that is laser-micro dissected specimens from formalin-fixed, paraffin-embedded (FFPE) tissue sections of patients with Parkinson’s disease. A preliminary experiment using the rat FFPE sections was revealed that the RNA integrity of formalin-fixed dopaminergic neurons is exacerbated by the adoption of the protocol for immunohistochemistry. This poor RNA integrity makes it more difficult to perform miRNA microarray analysis. To improve the RNA integrity, immunohistochemistry protocols may require further modifications.