Functional mapping of deep brain structures using microendoscope

Research Project

Project/Area Number	24650223
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Fusional brain recording science
Research Institution	Kyoto University
Principal Investigator	HAYASHI Yuichiro 京都大学, 医学(系)研究科(研究院), 助教 (90378737)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	カルシウムイメージング / 内視鏡 / 歯状回 / 海馬 / オプトジェネティクス / チャネルロドプシン / 共焦点顕微鏡
Outline of Final Research Achievements	To visualize neural activity in deep brain structures, I developed an endoscope system for fluorescence imaging. Gradient index (GRIN) lens-based endoscope was implanted in a mouse expressing GCaMP7 calcium indicator. The mouse was head-restrained and placed on a spherical treadmill. Using the endoscope, calcium activity of dentate granule cells was visualized. Cellular signals in the fluorescent images were extracted using principal and independent component analysis. Approximately 40 signals were extracted from the images and their signal-to-noise ratio were 4-10.