Developement of in vivo deep brain connection by fluorescence imaging

• Project/Area Number: 24650225
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Fusional brain recording science
• Research Institution: 独立行政法人国立精神・神経医療研究センター
• Principal Investigator: NORITAKA Ichinohe 独立行政法人国立精神・神経医療研究センター, 神経研究所 微細構造研究部, 部長 (00250598)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 線維連絡 / in vivo / 蛍光色素 / 機能・解剖連関 / in vivo 生体線維連絡 / 脳connection / 蛍光・定量トモグラフィ装置 / 超波長蛍光 / 生体内線維連絡可視化法 / 蛍光トレーサー / 脳深部イメージング / コレラトキシン

Research Abstract

We have succeded to develope that using in vivo fluorescence tomography equipment, CTB-Alexa 750 as a fiber tract tracer can be followed in vivo even in deep structures. Until now, our methods using CTB-Alexa 555 can only show coritical surface connections. This is the great development, especially, connection are concerned with deep brain region (e.g., amygdala, basal ganglia, hippocampus, thalamus, mideial prefrontal cortex, cingulate). Especially, function of connected area can be electrophysiologically examined.

Research Products

• [Journal Article] Characterization of claustral neurons by comparative gene expression profiling and dye-injection analyses (2014)
  • Author(s): Akiya Watakabe* , Noritaka Ichinohe, Kathleen S Rockland and Tetsuo Yamamori
  • Journal Title: Front. Syst. Neurosci Volume: なし
• [Journal Article] Marmoset monkeys evaluate third-party reciprocity (2014)
  • Author(s): Kawai, N., Yasue, M., Banno, T., & Ichinohe, N.
  • Journal Title: Biology Letters Volume: なし
• [Journal Article] Morphologic changes of dendritic spines of striatal neurons in the levodopa-induced dyskinesia model (2014)
  • Author(s): Nishijima H, Suzuki S, Kon T, Funamizu Y, Ueno T, Haga R, Suzuki C, Arai A, Kimura T, Suzuki C, Meguro R, Miki Y, Yamada J, Migita K, Ichinohe N, Ueno S, Baba M, Tomiyama M
  • Journal Title: Mov Disord Volume: 29 Suppl 3 Issue: 3 Pages: 336-343
  • DOI: 10.1002/mds.25826
  • Peer Reviewed
• [Journal Article] Developmental expression profiles of axon guidance signaling and the immune system in the marmoset cortex: potential molecular mechanisms of pruning of dendritic spines during primate synapse formation in late infancy and prepuberty (I) (2014)
  • Author(s): Sasaki T, Oga T, Nakagaki K, Sakai K, Sumida K, Hoshino K, Miyawaki I, Saito K, Suto F, Ichinohe N*
  • Journal Title: Biochem Biophys Res Commun Volume: 444 Issue: 3 Pages: 302-306
  • DOI: 10.1016/j.bbrc.2014.01.024
  • Peer Reviewed / Open Access
• [Journal Article] Developmental genetic profiles of glutamate receptor system, neuromodulator system, protector of normal tissue and mitochondria, and reelin in marmoset cortex: potential molecular mechanisms of pruning phase of spines in primate synaptic formation process during the end of infancy and prepuberty (II) (2014)
  • Author(s): Sasaki T, Oga T, Nakagaki K, Sakai K, Sumida K, Hoshino K, Miyawaki I, Saito K, Suto F, Ichinohe N
  • Journal Title: Biochem Biophys Res Commun Volume: 444 Issue: 3 Pages: 307-310
  • DOI: 10.1016/j.bbrc.2014.01.023
  • Peer Reviewed / Open Access
• [Journal Article] Postnatal development of layer III pyramidal cells in the primary visual, inferior temporal, and prefrontal cortices of the marmoset (2013)
  • Author(s): Oga T, Aoi H, Sasaki T, Fujita I, Ichinohe N.
  • Journal Title: Front Neural Circuits Volume: 8;7:31
  • Peer Reviewed
• [Journal Article] Pyramidal neurons in the superficial layers of rat retrosplenial cortex exhibit a late-spiking firing property (2013)
  • Author(s): Kurotani T, Miyashita T, Wintzer M, Konishi T, Sakai K, Ichinohe N, Rockland KS
  • Journal Title: Brain Struct Funct Volume: 218(1) Issue: 1 Pages: 239-254
  • DOI: 10.1007/s00429-012-0398-1
  • Peer Reviewed
• [Journal Article] Postnatal development of layer III pyramidal cells in the primary visual, inferior temporal, and prefrontal cortices of the marmoset (2013)
  • Author(s): Oga T, Aoi H, Sasaki T, Fujita I, Ichinohe N
  • Journal Title: Front Neural Circuits Volume: 7 Pages: 31-31
  • DOI: 10.3389/fncir.2013.00031
  • Peer Reviewed
• [Journal Article] Distinct feedforward and intrinsic neurons in posterior inferotemporal cortex revealed by in vivo connection imaging (2012)
  • Author(s): Ichinohe N, Borra E, Rockland K
  • Journal Title: Sci Rep. Volume: 2 Issue: 1 Pages: 934-934
  • DOI: 10.1038/srep00934
  • Peer Reviewed
• [Journal Article] Area-Specific Substratification of Deep Layer Neurons in the Rat Cortex (2012)
  • Author(s): Akiya Watakabe, Junya Hirokawa, Noritaka Ichinohe, Sonoko Ohsawa, Takeshi Kaneko, Kathleen S. Rockland, and Tetsuo Yamamori
  • Journal Title: The Journal of Comparative Neurology Volume: 520(16) Issue: 16 Pages: 3553-3573
  • DOI: 10.1002/cne.23160
  • Peer Reviewed
• [Book] 線維連絡に基づく他者意図認知の脳基盤(ブレインサイエンス・レビュー2012:編集:伊藤正男、川合述史) (2012)
  • Author(s): 一戸紀孝
  • Publisher: クバプロ
• [Remarks] 物体を認識する脳内神経伝達メカニズムを解明
  • URL: http://www.ncnp.go.jp/press/press_release121207.html