Elucidation of cell fate determination mechanism of pancreatic endocrine cells using in vivo imaging

• Project/Area Number: 24650228
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: University of Tsukuba
• Principal Investigator: TAKAHASHI Satoru 筑波大学, 医学医療系, 教授 (50271896)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 発生・分化 / 糖尿病 / 細胞・組織 / 応用動物 / 再生医学 / 発生

Research Abstract

In this study, it is intended to make clear that the fluctuation of the cell fate of pancreatic beta cells is one of the mechanisms of diabetes progression. We also want to reveal that the plasticity of pancreatic endocrine cells associated with the instability is related to insulin resistance and a precipitating factor for the disease. To this end, We want to establish evaluation systems using animal model a new real-time imaging techniques.

Research Products

• [Journal Article] Generation and Characterization of Ins1-cre-driver C57BL/6N for Exclusive Pancreatic Beta Cell-specific Cre-loxP Recombination (2014)
  • Author(s): Hasegawa Y, Daitoku Y, Mizuno S, Tanimoto Y, Mizuno-Iijima S, Matsuo M, Kajiwara N, Ema M, Oishi H, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K.
  • Journal Title: Exp Animal Volume: 63 Pages: 183-191
  • NAID: 130003391621
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/24770644
  • Peer Reviewed
• [Journal Article] Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination (2013)
  • Author(s): Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K-i.
  • Journal Title: Exp Animal Volume: 62 Pages: 295-304
  • NAID: 130003382716
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=Hasegawa+Y%2C+Daitoku+Y%2C+Sekiguchi+K
  • Peer Reviewed
• [Journal Article] In vivo monitoring of pancreatic β -cell mass and intrahepatic insulin gene activity in Ins1-luc BAC transgenic mice by bioluminescence imaging (2013)
  • Author(s): Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Ema M, Kudo T, Takahashi S
  • Journal Title: Plos One Volume: 8 Issue: 4 Pages: e60411-e60411
  • DOI: 10.1371/journal.pone.0060411
  • Peer Reviewed
• [Journal Article] In vivo monitoring of pancreatic β-cell mass and intrahepatic insulin gene activity in Ins1-luc BAC transgenic mice by bioluminescence imaging. (2013)
  • Author(s): Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Ema M, Kudo T, Takahashi S.
  • Journal Title: Plos One Volume: 8
  • Peer Reviewed
• [Journal Article] Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. (2013)
  • Author(s): Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Mizuno-Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K.
  • Journal Title: Experimental Animals Volume: 62 Pages: 295-304
  • NAID: 130003382716
  • Peer Reviewed
• [Journal Article] In vivo monitoring of pancreatic β-cell mass and intrahepatic insulin gene activity in Ins1-luc BAC transgenic mice by bioluminescence imaging. (2013)
  • Author(s): Katsumata T
  • Journal Title: Plos One. Volume: 8(4)
  • Peer Reviewed
• [Journal Article] Non-invasive monitoring ofβ-cell mass and fetalβ-cell genesis in mouse using bioluminescence imaging (2012)
  • Author(s): Sekiguchi Y, Owada J, Oishi H, Katsumata T, Ikeda R, Kudo T, Takahashi S.
  • Journal Title: Exp Animal Volume: 61 Pages: 445-451
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=Sekiguchi+Y%2C+Owada+J%2C+Oishi+H
  • Peer Reviewed
• [Journal Article] Non-invasive monitoring of β-cell mass and fetal β-cell genesis in mouse using bioluminescence imaging. (2012)
  • Author(s): Sekiguchi Y
  • Journal Title: Exp Animal. Volume: 61 Pages: 445-451
  • Peer Reviewed
• [Presentation] 膵内分泌細胞の発生・分化における大Maf群転写因子の役割 (2013)
  • Author(s): 大石久史、高橋 智
  • Organizer: 日本解剖学会
  • Place of Presentation: サンポートホール高松
  • Invited
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index.html
• [Remarks] 筑波大学 解剖学・発生学研究室
  • URL: http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index.html