| Project/Area Number |
24650229
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 再生 / イモリ / 肢 / 系譜解析 / 脱分化 / 幹細胞 / 筋 / 再生医学 / 動物 / 発生・再生 / 細胞・組織 / 遺伝子 |
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| Outline of Final Research Achievements |
We established a novel experimental system named ‘TGP line’ which enables us to investigate cellular and molecular mechanisms underlying limb regeneration in adult newts by combination of a highly efficient transgenic technique (TG) and tissue transplantation techniques (TP). Utilizing this system, here we carried out cell lineage tracing in the limb regeneration, and found that larval and adult newts regenerate their limbs by different cellular mechanisms. In addition, in the adult newt, we found that the key tissues of the limb regeneration, such as the skin, muscles, bones and nerves, rigidly give rise to the same type of tissues, with a few exceptions. These findings would certainly contribute to understanding of differences in regeneration-competency among vertebrates.
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