Single cell photoporation in mammalian embryos induced by a femtosecond laser
| Project/Area Number |
24650230
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TANAKA Mikiko 東京工業大学, 生命理工学研究科, 准教授 (40376950)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOKAWA Yohichiro 奈良先端科学技術大学院大学, 物質創成科学研究科, 准教授 (20448088)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | レーザー / 遺伝子 |
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| Outline of Final Research Achievements |
In this project, we demonstrate that the effective introduction of biomolecules into targeted single cells in mammalian embryos by photoporation using a femtosecond laser amplifier with a high pulse energy and a low repetition rate. Briefly, E9.0 mouse embryos with closed yolk sacs were cultured, and then injected ~10,000 Da biomolecules into the neural tube. Subsequently, laser pulses of 400 nJ were focused on the surface of the neural tube through a small slit made in the yolk sac. At 24 hours post-injection, biomolecules were successfully delivered to single neurons of mouse embryos. These results suggest that our single cell photoporation technique could be applied to a mammalian embryos.
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Report
(4 results)
Research Products
(18 results)
