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Single cell photoporation in mammalian embryos induced by a femtosecond laser

Research Project

Project/Area Number 24650230
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionTokyo Institute of Technology

Principal Investigator

TANAKA Mikiko  東京工業大学, 生命理工学研究科, 准教授 (40376950)

Co-Investigator(Kenkyū-buntansha) HOSOKAWA Yohichiro  奈良先端科学技術大学院大学, 物質創成科学研究科, 准教授 (20448088)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsレーザー / 遺伝子
Outline of Final Research Achievements

In this project, we demonstrate that the effective introduction of biomolecules into targeted single cells in mammalian embryos by photoporation using a femtosecond laser amplifier with a high pulse energy and a low repetition rate. Briefly, E9.0 mouse embryos with closed yolk sacs were cultured, and then injected ~10,000 Da biomolecules into the neural tube. Subsequently, laser pulses of 400 nJ were focused on the surface of the neural tube through a small slit made in the yolk sac. At 24 hours post-injection, biomolecules were successfully delivered to single neurons of mouse embryos. These results suggest that our single cell photoporation technique could be applied to a mammalian embryos.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (18 results)

All 2015 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (9 results) (of which Invited: 3 results) Remarks (7 results)

  • [Journal Article] レーザー技術を応用した発生学研究の新展開2013

    • Author(s)
      田中幹子
    • Journal Title

      Proceedings of the 79th Laser Materials Processing Conference

      Volume: 79 Pages: 31-35

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] バイオ分野に注目されるフェムト秒レーザー細胞プロセス2012

    • Author(s)
      細川陽一郎
    • Journal Title

      応用物理

      Volume: 82 Pages: 63-67

    • NAID

      10031140509

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] フェムト秒レーザーを用いた植物単細胞への巨大分子の導入2015

    • Author(s)
      片桐 大輔、坂口 さやか、米田 新、出村 拓、細川 陽一郎
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      東海大学湘南キャンパス
    • Year and Date
      2015-03-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Evaluation of trade-off between femtosecond laser manipulation and viability of plant cell2015

    • Author(s)
      Daisuke Katagiri, Sayaka Sakaguchi, Arata Yoneda, Taku Demura, Yoichiroh Hosokawa
    • Organizer
      PSROC 2015 Annual Meeting
    • Place of Presentation
      Hsinchu, Taiwan
    • Year and Date
      2015-01-28 – 2015-01-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] レーザー技術を応用した発生学研究の新展開2013

    • Author(s)
      田中幹子
    • Organizer
      第79回レーザー加工学講演会
    • Place of Presentation
      関西大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 植物試料へのフェムト秒レーザー分子導入のための圧力制御装置の開発2013

    • Author(s)
      新屋龍太郎, 坂口さや香, 細川陽一郎
    • Organizer
      第60回応用物理学会春季学術講演会
    • Place of Presentation
      神奈川工科大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 超短パルスレーザーによる高等動物胚へのDDS2012

    • Author(s)
      細川陽一郎
    • Organizer
      第51回日本生体医工学会大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] フェムト秒レーザーを用いた植物試料への単一細胞分子導入の検討

    • Author(s)
      新屋龍太郎,坂口さや香,細川陽一郎
    • Organizer
      第74回応用物理学会秋季学術講演会
    • Place of Presentation
      同志社大
    • Related Report
      2013 Research-status Report
  • [Presentation] Molecular Introduction into Single Plant Cell by a Femtosecond Laser Ablation

    • Author(s)
      Ryutaro Shinya, Sayaka Sakaguchi, Yoichiroh Hosokawa
    • Organizer
      The 12th International Conference on Laser Ablation
    • Place of Presentation
      Ischia, Italy
    • Related Report
      2013 Research-status Report
  • [Presentation] Femtosecond Laser-induced photoporation into Tabacco BY-2 cell

    • Author(s)
      Ryutaro Shinya, Hitoshi Endo, Takanori Iino, Sayaka Sakaguchi, Arata Yoneda, Taku Demura, Yoichiroh Hosokawa
    • Organizer
      The Physical Society of Republic of China (Taiwan) Annual Meeting 2014
    • Place of Presentation
      Taichung, Taiwan
    • Related Report
      2013 Research-status Report
  • [Presentation] フェムト秒レーザーによる高等脊椎動物胚の単一細胞への遺伝子導入

    • Author(s)
      田中幹子
    • Organizer
      超短パルスレーザー細胞プロセス研究会
    • Place of Presentation
      奈良先端大
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks] 東京工業大学大学院生命理工学研究科 田中幹子研究室 HP

    • Related Report
      2014 Annual Research Report
  • [Remarks] 奈良先端科学技術大学院大学 環境フォトニクススーパー研究グループ グリーンバイオナノ研究室 HP

    • URL

      http://mswebs.naist.jp/LABs/env_photo_greenbio/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京工業大学大学院生命理工学研究科田中幹子研究室 HP

    • Related Report
      2013 Research-status Report
  • [Remarks] 奈良先端科学技術大学院大学 グリーンバイオナノ(細川研究室)HP

    • Related Report
      2013 Research-status Report
  • [Remarks] 山形大学医学部越智陽城研究室 HP

    • Related Report
      2013 Research-status Report
  • [Remarks] 東京工業大学大学院生命理工学研究科生体システム専攻 田中(幹)研究室

    • Related Report
      2012 Research-status Report
  • [Remarks] 奈良先端科学技術大学院大学物質創成科学研究科 グリーンバイオナノ研究室

    • URL

      http://mswebs.naist.jp/LABs/env_photo_greenbio/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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