Establishment of a novel mutant mouse strain showing age-related pigmentation and epigenetic changes in the age-related pigmentation
Project/Area Number |
24650233
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory animal science
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Research Institution | Nagoya University |
Principal Investigator |
ISHIKAWA Akira 名古屋大学, 生命農学研究科, 准教授 (20211724)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 遺伝学 / 遺伝子 / マウス / エピジェネティクス / エピジェネティックス |
Outline of Final Research Achievements |
During development of a mixed strain carrying the Oca2p-cas (oculocutaneous albinism II; pink-eyed dilution castaneus) gene discovered from Indonesian wild mice, a novel spontaneous mutant showing age-related pigmentation was found. A line fixed for this mutant phenotype was established by selective breeding of the mutant phenotype. mRNA-sequencing analysis indicated no remarkable differences in expression levels of genes related to melanin synthesis between mutant and control mice. Whole-genome DNA methylation analysis identified two autosomes with high and low methylated regions. In vitro serum-free epidermal cell culture supplemented with L-tyrosine greatly induced the differentiation of melanocytes in mutant mice compared with controls.
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Report
(4 results)
Research Products
(5 results)