| Project/Area Number |
24650236
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Nara Medical University (2013)
Osaka University (2012) |
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Principal Investigator |
HORIE Kyoji 奈良県立医科大学, 医学部, 教授 (30333446)
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| Project Period (FY) |
2012-04-01 – 2013-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 発生工学 / ES細胞 / ゲノム / 遺伝子 / バイオテクノロジー / ハプロイド / ベクター |
|---|
| Research Abstract |
Embryonic stem cells are characterized by unlimited self-renewal capacity and pluripotecy to differentiate into various cell lineages. In depth analyses of these two characteristics are needed in order to apply ES cells toward regenerative medicine. In the present study, we applied gene trap strategy to recently developed mouse haploid ES cells and demonstrated feasibility of gene inactivation through insertional mutageneisis. A large scale application of our mutagenesis scheme would accelerate phenotype-based screen for the genes essential for self-renewal potential and maintenance of pluripotency
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