| Project/Area Number |
24650238
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
IMOTO Issei (TACHIBANA Issei) 徳島大学, 大学院・へルスバイオサイエンス研究部, 教授 (30258610)
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| Co-Investigator(Renkei-kenkyūsha) |
TAJIMA Atsushi 徳島大学, 大学院・へルスバイオサイエンス研究部, 准教授 (10396864)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | リサーチバイオリソース / ラット疾患モデル動物 / エクソーム / 次世代シーケンサー / 責任遺伝子変異 / ゲノム情報 / 連鎖解析 / ラット / 疾患モデル動物 |
|---|
| Research Abstract |
In order to identify genes responsible for phenotypes observed in disease-model rats and determine molecular mechanisms producing those phenotypes, we modified our self-made rat exon capture system, which can easily analyze larger sets of exons in a liquid phase manner. We constructed an effective pipeline to analyze next-generation sequencing data with larger information of polymorphism and gene annotation data in rats. Through combining those systems with linkage analyses, we constructed effective and systematic tools for rat genome analysis. We finally applied this tool to a newly established rat model with unique phenotype, and validated usefulness of our system by collecting candidate variations possibly responsible for its phenotype.
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