Development of a new method to generate the model animal of human chromosomal disorders
| Project/Area Number |
24650241
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Institute of Physical and Chemical Research (2013)
National Institute of Genetics (2012) |
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Principal Investigator |
TAMURA Masaru 独立行政法人理化学研究所, バイオリソースセンター, 開発研究員 (50370119)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マウス / 染色体異常疾患 / トリソミー / モデル動物 / CRE/loxP / トランスポゾン / モノソミー |
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| Research Abstract |
It is estimated that over 1% of human newborn have some kind of chromosomal anomalies including partial trisomy. Although incidence of chromosomal abnormalities is high, molecular mechanisms and causative genes of chromosomal disorders remain unclear, because we have no animal model of most part of chromosomal diseases. In this study, we challenged the development of the new method that created the model animal of chromosomal disorders using the CRE/loxP technology and local hopping system of transposon. We inserted the loxP and Tol2 transposon into the mouse genome by transgenic or homologous recombination techniques. We obtained several transgenic mouse lines and ES cell lines, and established the platform to generate the model animal of chromosomal disorders. In addition, we tried to develop the new phenotyping method using the micro computed tomography and contrast agents. These new techniques will help us to understand the molecular mechanism of human chromosomal disorders.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Over-dosage of Hand2 causes limb and heart defects in human chromosomal disorder, partial trisomy distal 4q2013
Author(s)
Tamura M., Hosoya M., Fujita M., Iida T., Amano T., Maeno A., Kataoka T., Otsuka T., Tanaka S, Tomizawa S. and Shiroishi T.
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Journal Title
Hum. Mol. Genet
Volume: 22
Issue: 12
Pages: 2471-2481
DOI
Related Report
Peer Reviewed
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[Presentation] X-Ray data (X-ray & micro-CT)2013
Author(s)
Masaru Tamura, Yasuyo Kozawa, Kazuhiro Hirota, Toshihiko Shiroishi, Shigeharu Wakan
Organizer
International Mouse Phenotyping Consortium Phenotyping Workshop
Place of Presentation
CNR Headquarters, Rome, Italy
Related Report
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[Presentation] X-Ray data (X-ray & micro-CT)2013
Author(s)
Tamura M., Kozawa Y., Hirota K., Shiroishi T. and Wakana S.
Organizer
International Mouse Phenotyping Consortium Phenotyping Workshop
Place of Presentation
CNR Headquarters (Rome, Italy)
Related Report
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