Development of humanized-liver mice using hepatocyte derived from pediatric liver/biliary tract diseases and investigation of pathological mechanism of liver/biliary tract diseases.
| Project/Area Number |
24650244
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
TANOUE Akito 独立行政法人国立成育医療研究センター, 薬剤治療研究部, 部長 (60301800)
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| Research Collaborator |
NAKAMURA Kazuaki 独立行政法人国立成育医療研究センター, 研究所薬剤治療研究部・実験薬理研究室, 室長 (80392356)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 疾患モデル / ヒト肝型マウス / 胆道閉鎖症 / 免疫不全肝障害マウス / 疾患肝細胞移植マウス / 病態モデル |
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| Research Abstract |
In this study, we tried for development of humanized-liver mice in which the liver is reconstitute with human hepatocyte derived from some liver/biliary tract diseases. We demonstrated that we could produce some humanized-liver mice which have disease derived hepatocyte. Especially, we tried for producing humanized-liver using hepatocyte derived from biliary atresia. We showed that hepatocyte derived from biliary atresia produced human albumin and expressed drug metabolism enzymes in mouse liver. These results indicated that hepatocytes have normal hepatic functions even in biliary atresia. The results of this study indicated the possibility for development of humanized-disease liver mice and these mice may be useful for investigating pathological mechanism of liver/biliary tract diseases.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Hepatocyte transplantation using the living donor reduced-graft in a baby with ornithine transcarbamylase deficiency : a novel source for hepatocytes2014
Author(s)
Enosawa S, Horikawa R, Yamamoto A, Sakamoto S, Shigeta T, Nosaka S, Fujimoto J, Tanoue A, Nakamura K, Umezawa A, Matsubara Y, Matsui A, Kasahara M
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Journal Title
Liver Transplantation
Volume: 20
Issue: 3
Pages: 391-393
DOI
Related Report
Peer Reviewed
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[Presentation] HEPATOCYTES TRANSPLANTATION FOR AN INFANT OF ORNITHINE TRANSCARBAMYLASE DEFICIENCY USING CELLS ISOLATED FROM LIVING DONOR REDUCED-GRAFT TISSUE2014
Author(s)
Matsui A, Horikawa R, Yamamoto A, Sakamoto S, Shigeta T, Nosaka S, Fujimoto J, Tanoue A, Nakamura K, Umezawa A, Matsubara Y, Kasahara M.
Organizer
47th Annual Meeting of The European Society for Paediatric Gastroenterology, Hepatology and Nutrition
Place of Presentation
The Jerusalem International Convention Center
Related Report
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[Presentation] HEPATOCYTES TRANSPLANTATION FOR AN INFANT OF ORNITHINE TRANSCARBAMYLASE DEFICIENCY USING CELLS ISOLATED FROM LIVING DONOR REDUCED-GRAFT TISSUE2014
Author(s)
Matsui A, Horikawa R, Yamamoto A, Sakamoto S, Shigeta T, Nosaka S, Fujimoto J, Tanoue A, Nakamura K, Umezawa A, Matsubara Y, Kasahara M
Organizer
47th Annual Meeting of The European Society for Paediatric Gastroenterology, Hepatology and Nutrition
Place of Presentation
The Jerusalem International Convention Centre
Related Report
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