Detection and analyses of unusual structure of stratum corneum

• Project/Area Number: 24650247
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Hirosaki University
• Principal Investigator: NAKAGAWA Kouichi 弘前大学, 保健学研究科, 教授 (00244393)
• Co-Investigator(Kenkyū-buntansha): SAWAMURA Daisuke 弘前大学, 大学院医学研究科, 教授 (60196334)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 生体情報 / 生体計測 / 皮膚角層 / 電子スピン共鳴 / イメージング / スピンプローブ / 皮膚科学 / スピンラベル / ESRイメージング / シミュレーション

Outline of Final Research Achievements

We investigated skin structure in relation to spin probe motions in various types of skin using continuous wave (CW) electron paramagnetic resonance (EPR) and 9 GHz EPR imaging. Spin probe (5-doxyl stearic acid) was used to investigate structural aspects of psoriasis vulgaris stratum corneum.

Research Products

• [Journal Article] Heavy-ion induced sucrose radicals investigated using EPR and UV spectroscopy (2015)
  • Author(s): K. Nakagawa, Y. Karakirova, N. D. Yordanov
  • Journal Title: J. Radiation Research Volume: in press Issue: 3 Pages: 405-412
  • DOI: 10.1093/jrr/rru108
  • Peer Reviewed / Open Access
• [Journal Article] Investigation of radical locations in various sesame seeds by CW EPR and 9 GHz EPR imaging (2014)
  • Author(s): K. Nakagawa and H. Hara
  • Journal Title: Free Radical Research Volume: 49 Issue: 1 Pages: 1-6
  • DOI: 10.3109/10715762.2014.967691
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Locations of radical species in black pepper seeds investigated by CW EPR and 9 GHz EPR imaging (2014)
  • Author(s): K. Nakagawa and B. Epel
  • Journal Title: Spectrochimica Acta Part A, Molecular & Biomolecular Spectroscopy Volume: 131 Pages: 342-346
  • DOI: 10.1016/j.saa.2014.04.100
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In Vivo Oxygen Imaging Workshop 2014参加報告 (2014)
  • Author(s): 中川公一
  • Journal Title: 電子スピンサイエンス Volume: 12 Pages: 113-114
• [Journal Article] Low dose dependence of sucrose radical generation (2014)
  • Author(s): Y. Sato, K. Nakagawa, and T. Takahashi
  • Journal Title: Proceeding of the 5th International Symposium on Radiation Emergency Volume: 39 Pages: 39-41
• [Journal Article] ESR Investigation of Sucrose Radicals Produced by 0.25-4.5 Gy Doses of X-ray Irradiation (2014)
  • Author(s): K. Nakagawa, K. Kobukai, and Y. Sato
  • Journal Title: J Radiat. Res. Volume: in press Issue: 4 Pages: 726-729
  • DOI: 10.1093/jrr/rru018
  • Peer Reviewed
• [Journal Article] Effects of Low Dose X-ray Irradiation of Eggshells on Radical Production (2014)
  • Author(s): K. Nakagawa
  • Journal Title: Free Radical Research Volume: in press Issue: 6 Pages: 679-683
  • DOI: 10.3109/10715762.2014.900174
  • Peer Reviewed
• [Journal Article] EPR spectroscopic investigation ofpsoriatic finger nails (2013)
  • Author(s): Nakagawa K, Minakawa S, Sawamura D
  • Journal Title: Skin Res Technol Volume: 19(4) Issue: 4 Pages: 450-453
  • DOI: 10.1111/srt.12068
  • Peer Reviewed
• [Journal Article] Stratum corneum structure of psoriasis vulgaris investigated by EPR spin-probe method (2013)
  • Author(s): K. Nakagawa, S. Minakawa, and D. Sawamura
  • Journal Title: Appl. Magn. Reson. Volume: 44 Issue: 8 Pages: 941-948
  • DOI: 10.1007/s00723-013-0453-y
  • Peer Reviewed
• [Journal Article] 関東支部 第2回油化学セミナー弘前 (2013)
  • Author(s): 中川公一
  • Journal Title: オレオサイエンス Volume: 13 Pages: 502-503
• [Journal Article] 北京で開催されたAPES 2012紀行 (2013)
  • Author(s): 中川公一
  • Journal Title: 電子スピンサイエンス Volume: 11
• [Presentation] Effects of 0.10-10 Gy X-ray Irradiation on Eggshell Radical Production (2014)
  • Author(s): K. Nakagawa
  • Organizer: The 9th Asia Pacific EPR/ESR Symposium (APES2014)
  • Place of Presentation: Nara Culture Center, Ｎａｒａ city, Nara, Ｊａｐａｎ
  • Year and Date: 2014-11-11 – 2014-11-15
• [Presentation] EPR Spin Probe Investigation of Surface Structure Analyses of Skin (2014)
  • Author(s): K. Nakagawa，Ｓ． Ｍｉｎａｋａｗａ， Ｄ． Ｓａｗａｍｕｒａ
  • Organizer: The 9th Asia Pacific EPR/ESR Symposium (APES2014)
  • Place of Presentation: Nara Culture Center, Ｎａｒａ city, Nara, Ｊａｐａｎ
  • Year and Date: 2014-11-11 – 2014-11-15
• [Presentation] 電子スピン共鳴(ESR)法による皮膚乾癬の研究 (2014)
  • Author(s): 中川公一、皆川智子、澤村大輔
  • Organizer: 第78回日本皮膚科学会東部支部学術大会
  • Place of Presentation: ホテル青森（青森県、青森市）
  • Year and Date: 2014-10-04 – 2014-10-05
• [Presentation] 電子スピン共鳴(ESR)法を用いた乾癬の角層構造の薬効に関する研究 (2014)
  • Author(s): 川村優太、中川公一、皆川智子、澤村大輔
  • Organizer: 第78回日本皮膚科学会東部支部学術大会
  • Place of Presentation: ホテル青森（青森県、青森市）
  • Year and Date: 2014-10-04 – 2014-10-05
• [Presentation] 低線量X-線照射で生ずるスクロースラジカルのESR法の研究 (2014)
  • Author(s): 中川公一, 佐藤 譲, 小武海 拳
  • Organizer: 平成26年度 化学系学協会東北大会
  • Place of Presentation: 山形大学工学部（山形県、米沢市）
  • Year and Date: 2014-09-19 – 2014-09-20
• [Presentation] ESR-スピンプローブ法による皮膚角層の構造に関する研究 (2014)
  • Author(s): 中川公一、皆川智子、澤村大輔
  • Organizer: 日本油化学会第53回年会
  • Place of Presentation: ロイトン札幌（北海道、札幌市）
  • Year and Date: 2014-09-10 – 2014-09-11
• [Presentation] EPR Imaging determines locations of radical species in black pepper seeds (2014)
  • Author(s): Kouichi Nakagawa and Boris Epel
  • Organizer: 1st Asian Conference on Oleo Science
  • Place of Presentation: Royton Sapporo, Sapporo city，Hokkaido, Ｊａｐａｎ
  • Year and Date: 2014-09-07 – 2014-09-10
• [Presentation] Detection and Analyses of Structural Changes of Various Stratum Corneum (2014)
  • Author(s): K. Nakagawa
  • Organizer: The 3rd International Conference and Exhibition on Cosmetology & Trichology
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2014-07-21 – 2014-07-23
  • Invited
• [Presentation] Electron Paramagnetic Resonance (EPR) Investigation of Skin Structure of Psoriasis Vulgaris (2014)
  • Author(s): Kouichi Nakagawa, Satoko Minakawa, and Daisuke Sawamura
  • Organizer: The 3rd International Conference and Exhibition on Cosmetology & Trichology
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2014-07-21 – 2014-07-23
• [Presentation] Effects of Low Dose X-ray Irradiation (0.10-10 Gy) of Eggshells on Radical Production (2014)
  • Author(s): K. Nakagawa
  • Organizer: The 2nd Awaji International Workshop on Electron Spin Science and Technology: Biological and Material Science oriented Application (2nd AWEST 2014)
  • Place of Presentation: Awaji Yumebutai International Conference Center, Awaji Island, Hyogo, Japan
  • Year and Date: 2014-06-15 – 2014-06-17
• [Presentation] EPR Spin Probe Investigation of Skin Structure (2014)
  • Author(s): Kouichi Nakagawa, Satoko Minakawa, and Daisuke Sawamura
  • Organizer: The 2nd Awaji International Workshop on Electron Spin Science and Technology: Biological and Material Science oriented Application (2nd AWEST 2014)
  • Place of Presentation: Awaji Yumebutai International Conference Center, Awaji Island, Hyogo, Japan
  • Year and Date: 2014-06-15 – 2014-06-17
• [Presentation] 角層細胞間脂質モデルの構造と特性 (2014)
  • Author(s): 箱田優也, 田中佳祐, 油井研一, 酒井健一, 酒井秀樹, 阿部正彦, 橋本悟, 中川公一, 坂本一民
  • Organizer: 第39回日本香粧品学会
  • Place of Presentation: 有楽町朝日ホール（東京都、千代田区）
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] 9 GHz ESR法による皮膚疾患と皮膚の構造解析 (2014)
  • Author(s): 中川公一
  • Organizer: 第74回分析化学討論会
  • Place of Presentation: 日本大学工学部（福島県、郡山市）
  • Year and Date: 2014-05-24 – 2014-05-25
• [Presentation] 尋常性乾癬のESR法による構造的解析 (2013)
  • Author(s): 中川公一、皆川智子、澤村大輔
  • Organizer: 第66回日本酸化ストレス学会学術集会
  • Place of Presentation: 愛知県名古屋市
• [Presentation] 9 GHz EPR Investigation of Skin Surface (2013)
  • Author(s): K. Nakagawa, S. Minakawa, and D. Sawamura
  • Organizer: The 1st Awaji International Workshop on Electron Spin Science and Technologu: Biological and Material Science Oriented Application
  • Place of Presentation: Awaji Island, Hyogo
  • Invited
• [Presentation] Skin Structure of Psoriasis Vulgaris Investigated by EPR Spin-Probe Method (2013)
  • Author(s): K. Nakagawa, S. Minakawa, and D. Sawamura
  • Organizer: The 55th ANNUAL ROCKY MOUNTAIN CONFERENCE ON MAGNETIC RESONANCE
  • Place of Presentation: Denver, Colorado, USA
• [Presentation] Sucrose Radicals Induced by Low Dose Irradiation with X-ray (2013)
  • Author(s): K. Nakagawa, K. Kobukai, Y. Sato, T. Takahashi
  • Organizer: The 55th ANNUAL ROCKY MOUNTAIN CONFERENCE ON MAGNETIC RESONANCE
  • Place of Presentation: Denver, Colorado, USA
• [Presentation] ESR法による皮膚乾癬の皮膚構造に関する研究 (2013)
  • Author(s): 中川公一、皆川智子、澤村大輔
  • Organizer: 日本油化学会第52回年会
  • Place of Presentation: 宮城県仙台市
• [Presentation] ESR Investigation of Sucrose Radicals Produced by Low Dose X-ray Irradiation (2013)
  • Author(s): Kouichi Nakagawa, Ken Kobukai, Yuzuru Sato, Takuto Takhashi
  • Organizer: International Symposium for the 70th Anniversary of the Tohoku Branch of the Chemical Society of Japan
  • Place of Presentation: Sendai, Ｍｉｙａｇｉ，Japan
• [Presentation] Low dose dependence of sucrose radical generation (2013)
  • Author(s): Y. Sato, T. Takahashi, K. Kobukai, and K. Nakagawa
  • Organizer: The 5th International Symposium on Radiation Emergency
  • Place of Presentation: Aomori-shi, Aomori, Japan
• [Presentation] 9 GHz EPR INVESTIGATION OF SKIN STRUCTURE OF PSORIASIS VULGARIS (2013)
  • Author(s): Kouichi Nakagawa, Satoko Minakawa, Daisuke Sawamura
  • Organizer: 2013 Biennial Meeting of Society for Free Radical Research Asia, Biennial Meeting of Society for Free Radical Research Asia
  • Place of Presentation: Tao-Yuan, Taiwan
• [Presentation] スクロースへの低線量X-線と重粒子線照射で生ずるラジカルのEPR解析 (2013)
  • Author(s): 中川公一、佐藤譲、高橋拓斗
  • Organizer: 第52回電子スピンサイエンス学会年会
  • Place of Presentation: 埼玉県さいたま市
• [Presentation] EPR-スピンプローブ法による乾癬の皮膚構造の研究 (2013)
  • Author(s): 中川公一、皆川智子、澤村大輔
  • Organizer: 第52回電子スピンサイエンス学会年会
  • Place of Presentation: 埼玉県さいたま市
• [Presentation] ESR法による皮膚角層脂質の構造解析 (2013)
  • Author(s): 中川公一
  • Organizer: 分析化学と化学分析の青森フォーラム2013
  • Place of Presentation: 青森県弘前市
  • Invited
• [Presentation] 9 GHz EPRイメージング法の医科学への応用：皮膚疾患と皮膚構造の解析, (2013)
  • Author(s): 中川公一
  • Organizer: 分子研研究会: 先端スピン計測技術による分子性物質研究の現状と展望
  • Place of Presentation: 愛知県岡崎市
• [Presentation] A 9 GHz EPR imager for thin materials, such as skin (2012)
  • Author(s): Kouichi Nakagawa, Yasunori Ohba, Boris Epel, and Hitoshi Hirata
  • Organizer: The 8th Asia Pacific EPR/ESR Symposium (APES2012)
  • Place of Presentation: Beijing, China
  • Invited
• [Presentation] EPR Investigation of Sucrose Radicals Produced by X-ray and Heavy-ion Irradiations (2012)
  • Author(s): K. Nakagawa, K. Kobukai, K. Nakatsukasa, and A. Hironaka
  • Organizer: The 8th Asia Pacific EPR/ESR Symposium (APES2012)
  • Place of Presentation: Beijing, China
• [Presentation] Skin Lipid Structure Investigated by EPR Spin-probe Method and a Possible EPR-Imaging Application (2012)
  • Author(s): K. Nakagawa, Y. Ohba
  • Organizer: World Congress on Oleo Sicence and 29th ISF Congress
  • Place of Presentation: Sasebo
• [Presentation] A 9 GHz EPR Imager: A Possible Application to Skin (2012)
  • Author(s): Kouichi Nakagawa, Yasunori Ohba, Boris Epel, and Hitoshi Hirata
  • Organizer: The 2nd International Symposium on Electron Spin Science (ISESS 2012)
  • Place of Presentation: Sendai
• [Presentation] さまざまなスクロースへの重粒子線とX-線照射で生ずるラジカルのEPR解析 (2012)
  • Author(s): 小武海 拳、中務 孝一、弘中 輝、中川公一
  • Organizer: 第51回電子スピンサイエンス学会年会
  • Place of Presentation: 札幌
• [Presentation] EPR法による皮膚乾癬の研究、 (2012)
  • Author(s): 中川公一、濱欠慎司、皆川智子、澤村大輔
  • Organizer: 第51回電子スピンサイエンス学会年会
  • Place of Presentation: 札幌
• [Presentation] 放射線照射で生ずるスクロースラジカルのEPR法による研究 (2012)
  • Author(s): 中川公一、小武海 拳
  • Organizer: 平成24年度 化学系学協会東北大会
  • Place of Presentation: 秋田
• [Presentation] 皮膜用9 GHz ESRイメージング装置の試作 (2012)
  • Author(s): 中川公一、大庭裕範、Boris Epel、平田 拓
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島
• [Book] Psoriasis: Types, Triggers and Treatment Strategies (2013)
  • Author(s): K. Nakagawa, and D. Sawamura
  • Total Pages: 230
  • Publisher: Nova Science Publishers, Inc.
• [Book] Psoriasis: Types, Triggers and Treatment Strategies (2013)
  • Author(s): K. Nakagawa and D. Sawamura
  • Publisher: Nova Science Publishers, Inc.
• [Remarks] 中川リサーチグループ
  • URL: http://www.hs.hirosaki-u.ac.jp/~nakagawa/
• [Remarks] researchmap (JST)
  • URL: http://researchmap.jp/read0186203/
• [Remarks] Kouichi Nakagawa (Google scholar)
  • URL: http://scholar.google.co.jp/citations?user=wqcm6r8AAAAJ&hl=ja
• [Remarks] 弘前大学大学院保健学研究科
  • URL: http://www.hs.hirosaki-u.ac.jp/kouhou/hokengaku/cms/index.cgi/kenkyu/r_housha/185
• [Remarks] 中川リサーチグループ
  • URL: http://www.hs.hirosaki-u.ac.jp/~nakagawa/
• [Remarks] 弘前大学大学院保健学研究科
  • URL: http://www.hs.hirosaki-u.ac.jp/kouhou/hokengaku/cms/index.cgi/kenkyu/r_housha/185
• [Remarks] 中川リサーチグループ
  • URL: http://www.hs.hirosaki-u.ac.jp/~nakagawa/
• [Remarks] 弘前大学大学院保健学研究科
  • URL: http://www.hs.hirosaki-u.ac.jp/kouhou/hokengaku/cms/index.cgi/kenkyu/r_housha/185
• [Patent(Industrial Property Rights)] 電子スピン共鳴装置 (2013)
  • Inventor(s): 中川公一
  • Industrial Property Rights Holder: 中川公一
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-07-10
  • Acquisition Date: 2014-01-21