| Project/Area Number |
24650249
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
JIMBO Yasuhiko 東京大学, 新領域創成科学研究科, 教授 (20372401)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脳・神経 / 神経変性疾患 / 細胞・組織 / 神経科学 / ナノバイオ / 分子認識 |
|---|
| Research Abstract |
Cell-culture substrates for visualizing generation and propagation of Alzheimer's neuro-degeneration processes were developed. Multiple micro-chambers made of PDMS for cell culture were integrated on a substrate with 64 embedded micro-electrodes. The micro-chambers were connected each other via parallel PDMS micro-tunnels. The height of the tunnel structure was designed as 5 um to avoid migration of cell bodies. Hippocampal neurons were taken from Wistar rat embryos and cultured on the substrates. Immuno-histochemical staining with beta3-tubulin, which was a specific marker for neuronal cytoskeleton, suggested that neurites were successfully guided into the micro-tunnels and reached the neighbor chamber. Ab was applied to one of the micro-chambers and the activity was evaluated by extracellular recording and intracellular Ca transients. The results suggested that excess elevation of Ca concentration was induced by Ab application, which might be one of the causes of neuro-degeneration.
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