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Cloning of LDL receptors responding to fluid shear stress

Research Project

Project/Area Number 24650250
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAMOTO Kimiko  東京大学, 医学(系)研究科(研究院), 講師 (00323618)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords流れ剪断応力 / LDLコレステロール / 動脈硬化 / 血管内皮細胞 / 乱流 / 完全長cDNAライブラリー / 発現クローニング
Research Abstract

LDL receptors, which were related to the onset of atherosclerosis caused vascular diseases such as the myocardial infarction or the cerebral infarction, were cloned. Although the pathological findings, which the atherosclerosis frequently appears on the endothelial cells exposed to turbulent fluid shear stress in the vascular bifurcation, were widely known, the details of the molecular mechanism were not fully apparent. Two kinds of clones that the mRNA and protein expression levels were down-regulated in response to laminar shear stress whereas those expression levels were up-regulated in response to turbulent shear stress were identified in human vascular endothelial cells by using a full-length cDNA library. The immunostaining study was confirmed that these two kinds of protein were expressed in human atherosclerotic lesions. These results indicate that the molecules discovered in this study play an important role in the mechanism of the onset of atherosclerosis.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (38 results)

All 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (24 results) (of which Invited: 16 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Endothelial cell and model membranes respond to shear stress by rapidly decreasing the order of their lipid phases.2013

    • Author(s)
      K. Yamamoto and J. Ando
    • Journal Title

      J. Cell Sci.

      Volume: 126 Issue: 5 Pages: 1227-1234

    • DOI

      10.1242/jcs.119628

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Flow detection and calcium signaling in vascular endothelial cells.2013

    • Author(s)
      J. Ando and K. Yamamoto
    • Journal Title

      Cardiovasc. Res.

      Volume: 99 Issue: 2 Pages: 260-268

    • DOI

      10.1093/cvr/cvt084

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor.2013

    • Author(s)
      Y. Abe, Y. Ozaki, J. Kasuya, K. Yamamoto, J. Ando, R. Sudo, M. Ikeda and K. Tanishita
    • Journal Title

      PLoS ONE

      Volume: 8(12) Issue: 12 Pages: 1-12

    • DOI

      10.1371/journal.pone.0082085

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic imaging of flow-induced endothelial cell responses2013

    • Author(s)
      山本希美子、安藤譲二
    • Journal Title

      Japanese Journal of Thrombosis and Hemostasis

      Volume: 24 Issue: 6 Pages: 569-575

    • DOI

      10.2491/jjsth.24.569

    • NAID

      130003393319

    • ISSN
      0915-7441, 1880-8808
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluid shear stress induces differentiation of circulating phenotype endothelial progenitor cells.2012

    • Author(s)
      S. Obi, H. Masuda, T. Shizuno, A. Sato, K. Yamamoto, J. Ando, Y. Abe and T. Asahara
    • Journal Title

      Am. J. Physiol. Cell Physiol.

      Volume: 303 Issue: 6 Pages: 595-606

    • DOI

      10.1152/ajpcell.00133.2012

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Arterial shear stress augments the differentiation of endothelial progenitor cells adhered to VEGF-bound surfaces.2012

    • Author(s)
      Y. Suzuki, K. Yamamoto, J. Ando, K. Matsumoto, T. Matsuda
    • Journal Title

      Biochem. Biopys. Res. Commun.

      Volume: 423 Issue: 1 Pages: 91-97

    • DOI

      10.1016/j.bbrc.2012.05.088

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differentiation of circulating endothelial progenitor cells induced by shear stress.2012

    • Author(s)
      S. Obi, K. Yamamoto, J. Ando, H. Masuda, T. Asahara.
    • Journal Title

      2012 International Symposium on Micro-NanoMechatronics and Human Science

      Volume: - Pages: 54-58

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Real-time imaging of flow-induced ATP release and Ca2+ signaling in vascular endothelial cells.2012

    • Author(s)
      K. Yamamoto and J. Ando.
    • Journal Title

      Biorheology

      Volume: 49 Pages: 115-116

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Arterial shear stress accelerates the differentiation of endothelial progenitor cells adhered on VEGF-bound surface.2012

    • Author(s)
      Y. Suzuki, K. Yamamoto, J. Ando, K. Matsumoto, and T. Matsuda.
    • Journal Title

      J. Tissue Eng. Regen. Med.

      Volume: 6 Pages: 241-241

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 血管のメカノバイオロジー:血管内皮細胞の血流センシングと循環調節2012

    • Author(s)
      安藤 譲二、山本 希美子
    • Journal Title

      細胞工学

      Volume: 31(6) Pages: 1017-1023

    • Related Report
      2012 Research-status Report
  • [Presentation] 血流刺激による血管前駆細胞の分化誘導2014

    • Author(s)
      山本希美子、安藤譲二
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Dynamic Mechano- transduction in Vascular Cells2013

    • Author(s)
      K. Yamamoto.
    • Organizer
      Seminar in the TEMASEK Life Sciences Laboratory, and 7^<th> International Conference on clinical Hemorheology
    • Place of Presentation
      National University Singapore, Singapore, Istanbul
    • Year and Date
      2013-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Flow detection and calcium signaling in vascular endothelial cells2013

    • Author(s)
      K. Yamamoto.
    • Organizer
      Lecture in the Department of Bioengineering
    • Place of Presentation
      Imperial College London, London
    • Year and Date
      2013-09-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Vascular endothelial cells respond to shear stress by rapidly decreasing the order of their lipid phases2013

    • Author(s)
      K. Yamamoto and Joji Ando
    • Organizer
      35^<th> Annual International Conference of the IEEE Engineering in Medicine and Biology Society in conjunction with 52^<nd> Annual Conference of Japanese Society for Medical and Biological Engineering (JSMBE)
    • Place of Presentation
      Osaka, (Osaka International Convention Center)
    • Year and Date
      2013-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Dynamic Mechanotransduction in Vascular Cells2013

    • Author(s)
      Kimiko Yamamoto
    • Organizer
      Seminar in the TEMASEK Life Sciences Laboratory, National University Singapore
    • Place of Presentation
      Singapore, Singapore,
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Flow detection and calcium signaling in vascular endothelial cells2013

    • Author(s)
      Kimiko Yamamoto
    • Organizer
      Lecture in the Department of Bioengineering. Imperial College London
    • Place of Presentation
      London, UK
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Vascular endothelial cells respond to shear stress by rapidly decreasing the order of their lipid phases2013

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society in conjunction with 52nd Annual Conference of Japanese Society for Medical and Biological Engineering
    • Place of Presentation
      Osaka
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Dynamic cellular mechanotransduction and nano-biomechanics2013

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      8th Aso International Meeting
    • Place of Presentation
      Kumamoto
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 胚性幹細胞のメカノバイオロジー2013

    • Author(s)
      山本希美子、安藤譲二
    • Organizer
      第31回日本ヒト細胞学会学術集会
    • Place of Presentation
      所沢
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 血管内皮細胞における血流刺激のメカノセンシング2013

    • Author(s)
      山本希美子、安藤譲二
    • Organizer
      第13回日本NO学会学術集会
    • Place of Presentation
      那覇
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Vascular Mechanobiology: Flow detection and calcium signaling in endothelial cells2013

    • Author(s)
      山本希美子、安藤譲二
    • Organizer
      第36回日本バイオレオロジー学会年会 日韓合同シンポジウム
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Vascular endothelial cells respond to shear stress by rapidly decreasing the order of their lipid phases2013

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society in conjunction with 52nd Annual Conference of JSMBE
    • Place of Presentation
      Osaka (Osaka International Convention Center)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Dynamic cellular mechanotransduction and nano-biomechanics2013

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      8th Aso International Meeting (AIM)
    • Place of Presentation
      Kumamoto(Hotel Greenpia Mimamiaso)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 血管内皮P2X4受容体を介した循環機能調節・Endothelial P2X4 receptor-mediated regulation of vascular functions2013

    • Author(s)
      山本希美子、安藤譲二
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡 (福岡国際会議場)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Shear- stress-mediated control of vascular functions through endothelial ATP release and P2X4 ion channels2012

    • Author(s)
      K. Yamamoto and Joji Ando
    • Organizer
      14^<th> International Congress of Biorheology
    • Place of Presentation
      Istanbul
    • Year and Date
      2012-07-04
    • Related Report
      2013 Final Research Report
  • [Presentation] ATP-gated P2X4 ion channel serve as transducers for shear stress mechanotransduction in vascular endothelial cells2012

    • Author(s)
      K. Yamamoto and Joji Ando
    • Organizer
      Purine 2012 in Fukuoka : International Symposium on Purinergic Signaling in New Strategy of Drag Discovery
    • Place of Presentation
      Fukuoka, Centennial Hall Kyushu University School of Medicine
    • Year and Date
      2012-06-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Differentiation of circulating endothelial progenitor cells induced by shear stress2012

    • Author(s)
      Syotaro Obi, Kimiko Yamamoto, Joji Ando, Haruchika Masuda, and Takayuki Asahara,
    • Organizer
      MHS2012
    • Place of Presentation
      名古屋(名古屋大学)
    • Related Report
      2012 Research-status Report
  • [Presentation] Chemiluminescence imaging of flow-induced ATP release at caveolae in vascular endothelial cells2012

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋(名古屋大学)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Vascular cells responses to biomechanical forces2012

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      9th International Conference on Flow Dynamics
    • Place of Presentation
      仙台(Hotel Metropolitan Sendai)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Shear-stress-mediated control of vascular functions through endothelial ATP release and P2X4 ion channels2012

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      14th International Congress of Biorheology and 7th International Conference on clinical Hemorheology
    • Place of Presentation
      トルコ(Istanbul)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] in situ血管内皮前駆細胞捕捉・分化誘導バイオインターフェイスの開発:接着細胞へのシェアストレスの影響2012

    • Author(s)
      鈴木芳典、山本希美子、安藤譲二、松本邦夫、松田武久
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] ATP-gated P2X4 ion channel serve as transducers for shear stress mechanotransduction in vascular endothelial cells2012

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      Purine 2012 in Fukuoka: International Symposium on Purinergic Signaling in New Strategy of Drag Discovery
    • Place of Presentation
      福岡 (Centennial Hall Kyushu University School of Medicine)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Visualization of flow-induced ATP release and triggering of Ca2+ waves at caveolae in vascular endothelial cells2012

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Organizer
      World Congress 2012, Medical Physics and Biomedical Engineering
    • Place of Presentation
      中華人民共和国(Beijing)
    • Related Report
      2012 Research-status Report
  • [Presentation] 血管のメカノバイオロジー:血流刺激に対する内皮細胞応答2012

    • Author(s)
      山本希美子、安藤譲二
    • Organizer
      第51回日本生体医工学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] Endothelial mechanotransduction and its role in the control of vascular functions in Shu Chien and Shi Yongde (eds.)2012

    • Author(s)
      K. Yamamoto and J. Ando.
    • Publisher
      Recent Advances in Mechanobiology, Shanghai Science and Technological Literature Publishing House, Shanghai
    • Related Report
      2013 Final Research Report
  • [Book] Endothelial mechanotransduction and its role in the control of vascular functions2012

    • Author(s)
      Kimiko Yamamoto and Joji Ando
    • Publisher
      Shanghai Science and Technological Literature Publishing House
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/bme/

    • Related Report
      2013 Final Research Report
  • [Remarks] 東京大学 大学院医学系研究科 医用生体工学講座 システム生理学

    • URL

      http://square.umin.ac.jp/bme/

    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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