| Project/Area Number |
24650258
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Mie University |
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Principal Investigator |
HORIUCHI Takashi 三重大学, 工学(系)研究科(研究院), 教授 (10201758)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Keiichi 三重大学, 大学院工学研究科, 准教授 (70252343)
NOMURA Shinsuke 三重大学, 医学部附属病院, 客員教授 (20198625)
ISHIKAWA Eiji 三重大学, 医学部附属病院, 講師 (10362352)
MURATA Tomohiro 三重大学, 医学部附属病院, 助教 (90508532)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 腹膜透析 / 腹膜中皮細胞 / 再生医療 / 細胞分離 / 細胞老化 / 形質変換 / 抗酸化能 / 細胞間結合 |
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| Research Abstract |
It was found that normal peritoneal mesothelial cells, epithelial-mesenchymal transition cells (EMT) and senescence cells are mixed in peritoneal dialysis effluent. Therefore, we separated those cell populations by percoll density centrifugation method and performed characterization of each cell population. As a result, density distributions of peritoneal dialysis effluent derived HPMC(PDE-HPMC) were obtained, indicating that transepithelial resistance of those cells are different. According to these result a strategy which the normal cells can be separated from the cell populations by percoll density centrifugation method was established.
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