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Pacemaking mechanism of human SA node cell using human iPS-derived cardiomyocytes

Research Project

Project/Area Number 24650260
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionUniversity of Fukui (2013)
Kyoto University (2012)

Principal Investigator

TAKEUCHI Ayako  福井大学, 医学部, 特命助教 (00378704)

Research Collaborator HIMENO Yukiko  立命館大学, 日本学術振興会, 特別研究員
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsiPS細胞 / ペースメーカー / 数理モデル解析
Research Abstract

The purpose of the present study was to clarify pacemaking mechanisms of human cardiac pacemaker cells, SA node cells. In order to achieve the goals, I performed cellular physiological experiments using human iPS-derived cardiomyocytes and mathematical simulations. Several combinations of chemicals enhanced proliferation of human iPS-derived cardiomyocytes, with similar electrophysiological characteristics. Theoretical analyses suggested that coupling of mitochondrial and sarcoplasmic reticulum Ca dynamics is important for pacemaking, and that contribution of mitochondrial Ca transporter is mechanism dependent.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (41 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (32 results) (of which Invited: 3 results) Book (4 results) Remarks (3 results)

  • [Journal Article] Identification of chemicals inducing cardiomyocyte proliferation in developmental stage-specific manner with pluripotent stem cells2013

    • Author(s)
      Uosaki H, Magadum A, Seo K, Fukushima H, Takeuchi A, Nakagawa Y, Moyes KW, Narazaki G, Kuwahara K, Laflamme M, Matsuoka S, Nakatsuji N, Nakao K, Kwon C, Kass DA, Engel FB, Yamashita JK
    • Journal Title

      Circ Cardiovasc Genet

      Volume: 6(6) Issue: 6 Pages: 624-33

    • DOI

      10.1161/circgenetics.113.000330

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondria Na+-Ca2+exchange in cardiomyocytes and lymphocytes2013

    • Author(s)
      Kim B, Takeuchi A, Koga O, Hikida M, Matsuoka S
    • Journal Title

      Advances in Experimental Medicine and Biology

      Volume: 961 Pages: 193-201

    • DOI

      10.1007/978-1-4614-4756-6_16

    • ISBN
      9781461447559, 9781461447566
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Mitochondrial Na-Ca exchanger NCLX-mediated mitochondria-sarcoplasmic reticulum Ca crosstalk and cardiomyocyte automaticity2014

    • Author(s)
      Takeuchi A, Matsuoka S
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      シンポジウム、鹿児島
    • Year and Date
      2014-03-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Mitochondrial Na-Ca exchanger NCLX-mediated mitochondria-sarcoplasmic reticulum Ca crosstalk and cardiomyocyte automaticity2014

    • Author(s)
      Takeuchi A, Matsuoka S
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学 (鹿児島市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 洞房結節細胞自動能におけるミトコンドリアNCLXの寄与―シミュレーション解析2013

    • Author(s)
      竹内綾子、松岡達
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎
    • Year and Date
      2013-11-27
    • Related Report
      2013 Final Research Report
  • [Presentation] システム生理学研究による細胞内イオンダイナミクス―細胞生理機能連関の包括的理解2013

    • Author(s)
      竹内綾子、松岡達
    • Organizer
      第7回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2013-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Theoretical analysis of mitochondrial NCX (NCLX)-mediated regulation of cardiac automaticity2013

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of mitochondrial NCX on CXCL12-induced chemotaxix in A20 B lymphocytes2013

    • Author(s)
      Kim B, Takeuchi A, Matsuoka S
    • Organizer
      57th Biophysical Society Annual Meeting
    • Place of Presentation
      フィラデルフィア、アメリカ合衆国
    • Year and Date
      2013-02-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Prolongation of beating rate caused by reduction of NCLX, a mitochondrial Na^+-Ca^<2+> exchanger, in HL-1 cardiomyocytes2013

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      57th Biophysical Society Annual Meeting
    • Place of Presentation
      フィラデルフィア、アメリカ合衆国
    • Year and Date
      2013-02-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Simulation analyses of the role of NCLX, a mitochondrial Na^+-Ca^<2+> exchanger, on the regulation of automaticity in sinoatrial node cells2013

    • Author(s)
      Takeuchi A, Matsuoka S
    • Organizer
      2013 Cardiac Physiome Workshop
    • Place of Presentation
      バーハーバー、アメリカ合衆国
    • Related Report
      2013 Final Research Report
  • [Presentation] Theoretical analysis of mitochondrial NCX-mediated regulation of automaticity in sinoatrial node cells2013

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      The 2nd HD Physiology International Symposium : Multi-Level Systems Biology
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Mitochondrial NCX controls directional migration of B lymphocytes2013

    • Author(s)
      Kim B, Takeuchi A, Matsuoka S
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 洞房結節細胞自動能におけるミトコンドリアNCLXの寄与―シミュレーション解析2013

    • Author(s)
      竹内綾子,松岡達
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      生理学研究所 (岡崎市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] システム生理学研究による細胞内イオンダイナミクス―細胞生理機能連関の包括的理解2013

    • Author(s)
      竹内綾子, 松岡達
    • Organizer
      第7回 次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      東北大学片平キャンパス (仙台市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Simulation analyses of the role of NCLX, a mitochondrial Na+-Ca2+ exchanger, on the regulation of automaticity in sinoatrial node cells2013

    • Author(s)
      Takeuchi A, Matsuoka S
    • Organizer
      2013 Cardiac Physiome Workshop
    • Place of Presentation
      Bar Harbor (アメリカ合衆国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Theoretical analysis of mitochondrial NCX-mediated regulation of automaticity in sinoatrial node cells2013

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      The 2nd HD Physiology International Symposium: Multi-Level Systems Biology
    • Place of Presentation
      明治安田生命ビル4階 My Plazaホール (東京都千代田区)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Mitochondrial NCX controls directional migration of B lymphocyte2013

    • Author(s)
      Kim B
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Theoretical analysis of mitochondrial NCX(NCLX)!mediated regulation of cardiac automaticity2013

    • Author(s)
      Takeuchi A
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Role of mitochondrial NCX on CXCL12-induced chemotaxis in A20 B lymphocytes2013

    • Author(s)
      Kim B
    • Organizer
      57th Biophysical Society Annual Meeting
    • Place of Presentation
      フィラデルフィア
    • Related Report
      2012 Research-status Report
  • [Presentation] Prolongation of beating rate caused by reduction of NCLX, a mitochondrial Na+-Ca2+ exchanger, in HL-1 cardiomyocytes2013

    • Author(s)
      Takeuchi A
    • Organizer
      57th Biophysical Society Annual Meeting
    • Place of Presentation
      フィラデルフィア
    • Related Report
      2012 Research-status Report
  • [Presentation] 拍動培養心筋細胞HL-1におけるミトコンドリアNa^+-Ca^<2+>交換輸送体NCLXの役割2012

    • Author(s)
      竹内綾子,金鳳柱、松岡達
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎
    • Year and Date
      2012-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Fine-Tuning of Cardiac Automaticity by Mitochondria Na^+-Ca^<2+> Exchange (NCLX)2012

    • Author(s)
      Matsuoka S, Takeuchi A, Kim B
    • Organizer
      2012 Cardiac Physiome Workshop
    • Place of Presentation
      サンディエゴ、アメリカ合衆国
    • Year and Date
      2012-10-31
    • Related Report
      2013 Final Research Report
  • [Presentation] ミトコンドリアNa-Ca交換輸送体NCLXを介した心筋細胞自動能制御2012

    • Author(s)
      竹内綾子,金鳳柱、松岡達
    • Organizer
      第29回日本心電学会学術集会
    • Place of Presentation
      千葉
    • Year and Date
      2012-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Inhibition of a mitochondrial Na^+-Ca^<2+> exchanger, NCLX, slows beating rate of cardiomyocyte2012

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S
    • Organizer
      第6回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Mechanisms underlying NCLX reduction-mediated slowing of cardiomyocyte beating rate2012

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S.
    • Organizer
      Cardiac Physiome Workshop
    • Place of Presentation
      サンディエゴ、アメリカ合衆国
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of Ca clock -mediated cardiac automaticity by mitochondrial NCX (NCLX)2012

    • Author(s)
      Takeuchi A, Kim B, Matsuoka S.
    • Organizer
      12th Symposium of the European Calcium Society
    • Place of Presentation
      トゥールーズ、フランス
    • Related Report
      2013 Final Research Report
  • [Presentation] 心筋細胞の自動能発生におけるミトコンドリアNa^+-Ca^<2+>交換輸送体NCLXの役割2012

    • Author(s)
      竹内綾子,金鳳柱、松岡達
    • Organizer
      第7回トランスポーター研究会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] Inhibition of a mitochondrial Na+-Ca2+ exchanger, NCLX, slows beating rate of cardiomyocyte2012

    • Author(s)
      Takeuchi A
    • Organizer
      第6回次世代を担う若手医療薬科学シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 拍動培養心筋細胞HL-1におけるミトコンドリアNa+-Ca2+交換輸送体NCLXの役割2012

    • Author(s)
      竹内綾子
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Fine-Tuning of Cardiac Automaticity by Mitochondria Sodium-Calcium Exchange2012

    • Author(s)
      Matsuoka S
    • Organizer
      Cardiac Physiome Workshop
    • Place of Presentation
      サンディエゴ
    • Related Report
      2012 Research-status Report
  • [Presentation] Mechanisms underlying NCLX reduction-mediated slowing of cardiomyocyte beating rate2012

    • Author(s)
      Takeuchi A
    • Organizer
      Cardiac Physiome Workshop
    • Place of Presentation
      サンディエゴ
    • Related Report
      2012 Research-status Report
  • [Presentation] ミトコンドリアNa-Ca交換輸送体NCLXを介した心筋細胞自動能制御2012

    • Author(s)
      竹内綾子
    • Organizer
      第29回 日本心電学会学術集会
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of “Ca clock”-mediated cardiac automaticity by mitochondrial NCX (NCLX)2012

    • Author(s)
      Takeuchi A
    • Organizer
      12th Symposium of the European Calcium Society
    • Place of Presentation
      トゥールーズ
    • Related Report
      2012 Research-status Report
  • [Presentation] 心筋細胞の自動能発生におけるミトコンドリアNa+-Ca2+交換輸送体NCLXの役割2012

    • Author(s)
      竹内綾子
    • Organizer
      第7回トランスポーター研究会
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Book] 心室筋細胞興奮収縮連関. In生理学実習書2013

    • Author(s)
      松岡達、竹内綾子
    • Total Pages
      296
    • Publisher
      日本生理学会教育委員会監修,南江堂
    • Related Report
      2013 Final Research Report
  • [Book] 生理学実習書2013

    • Author(s)
      松岡達、竹内綾子
    • Total Pages
      296
    • Publisher
      南江堂
    • Related Report
      2013 Annual Research Report
  • [Book] Giant Patch and Macro Patch. In Patch Clamp Techniques-From beginning to advanced protocols(edited by Okada Y)(Chapter 14)2012

    • Author(s)
      Matsuoka S, Takeuchi A.
    • Total Pages
      439
    • Publisher
      Springer Protocols, Tokyo
    • Related Report
      2013 Final Research Report
  • [Book] Patch Clamp Techniques-From beginning to advanced protocols-. Chapter 14. Giant Patch and Macro Patch.2012

    • Author(s)
      Matsuoka S
    • Publisher
      Springer Protocols
    • Related Report
      2012 Research-status Report
  • [Remarks] 福井大学医学部統合生理学ホームページ

    • URL

      http://isphysio.med.u-fukui.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks] 福井大学医学部統合生理学ホームページ

    • URL

      http://seiri2.med.lab.u-fukui.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks] 福井大学医学部統合生理学ホームページ

    • URL

      http://isphysio.med.u-fukui.ac.jp/index.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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