Manipulation of high-density lipoprotein structure and function for development of gene delivery carrier

• Project/Area Number: 24650263
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyoto University
• Principal Investigator: MURAKAMI Tatsuya 京都大学, 物質-細胞統合システム拠点, 准教授 (90410737)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 遺伝子キャリア / ドラッグデリバリー / ナノバイオテクノロジー

Research Abstract

Our purpose in this study is to develop nanomaterials capable of delivering therapeutic gene to desired sites, such as cancer tissues, by utilizing natural nanomaterials in our body. High-density lipoprotein (HDL) is a natural nanomaterial that delivers lipids from peripheral tissues to the liver in our body. Due to its physiological functions, HDL have a propensity to accumulate in liver via HDL receptor recognition of apoA-I moiety in HDL. In order to weaken this recognition in the liver and then to redirect HDL to desired sites, we designed 9 apoA-I deletion mutants and succeeded in preparation of 8 HDL mutants. Among them, a few HDL mutants were found to show decreased affinity to the HDL receptor.

Research Products

• [Journal Article] Photothermal ablation of tumor cells using a single-walled carbon nanotube?peptide composite (2014)
  • Author(s): Tanaka, H.; Zhou, S.; Kawakami, S.; Yamashita, F.; Murakami, T.; Umeyama, T.; Imahori, H.; Hashida, M.
  • Journal Title: Journal of Controlled Release Volume: 173 Pages: 59-66
  • NAID: 120005474483
• [Journal Article] Development of anionic bubble lipopolyplexes for efficient and safe gene transfection with ultrasound exposure in mice (2014)
  • Author(s): Hashida, Y.; Tanaka, H.; Zhou, S.; Kawakami, S.; Ymashita, F.; Murakami, T.; Umeyama, T.; Imahori, H.: Hashida, M.
  • Journal Title: Journal of Controlled Release Volume: 176 Issue: 1 Pages: 24
  • DOI: 10.1016/j.jconrel.2013.12.023
  • NAID: 120006986739
  • Peer Reviewed
• [Journal Article] Caboxylated SiO2-coated alpha-Fe nanoparticles: towards a versatile platform for biomedical applications (2013)
  • Author(s): Kohara, K.; Yamamoto, S.; Seinberg, L.; Murakami, T.; Tsujimoto, M.; Ogawa, T.; Kurata, H.; Kageyama, H.; Takano, M.
  • Journal Title: Chemical Communications Volume: 49 Issue: 25 Pages: 2563-2565
  • DOI: 10.1039/c3cc39055a
  • Peer Reviewed
• [Journal Article] Helicity Selective Photoreaction of Single-walled Carbon Nanotubes with Organosulfur Compounds in the Presence of Oxygen (2013)
  • Author(s): Maeda, Y.; Higo, J.; Amagai, Y.; Matsui, J.; Ohkubo, K.; Yoshigoe, Y.; Hashimoto, M.; Eguchi, K.; Yamada, M.; Hasegawa, T.; Sato, Y.; Zhou, J.; Lu, J.; Miyashita, T.; Fukuzumi, S.; Murakami, T.; Tohji, K.; Nagase, S; Akasaka, T.
  • Journal Title: J. Am. Chem. Soc. Volume: 135 Issue: 16 Pages: 6356-6362
  • DOI: 10.1021/ja402199n
  • Peer Reviewed
• [Journal Article] Fabrication of Pure Nanodrugs of Podophyllotoxin Dimer and Their Anticancer Activity (2013)
  • Author(s): Y. Ikuta, Y. Koseki, T. Murakami, M. Ueda, H. Oikawa, H. Kasai
  • Journal Title: Chem. Lett. Volume: 48 Issue: 8 Pages: 900-901
  • DOI: 10.1246/cl.130224
  • NAID: 10031189289
  • Peer Reviewed
• [Journal Article] Exclusive Photothermal Heat Generation by a Gadolinium Bis(naphthalocyanine) Complex and Inclusion into Modified High-Density Lipoprotein Nanocarriers for Therapeutic Applications (2013)
  • Author(s): Mathew S, Murakami T, Nakatsuji H, Okamoto H, Morone N, Heuser JE, Hashida M, Imahori H.
  • Journal Title: ACS Nano. Volume: 7(10) Issue: 10 Pages: 8908-16
  • DOI: 10.1021/nn403384k
  • Peer Reviewed
• [Journal Article] Photodynamic and photothermal effecrts of semiconducting and metallic-enriched single-walled carbon nanotubes (2012)
  • Author(s): Murakami, T., Nakatsuji, H., Inada, M., Matoba, Y., Umeyama, T., Tsujimoto, M. , Isoda, S., Hashida, M., Imahori, H.
  • Journal Title: Journal of the American Chemical Society Volume: 134 Issue: 43 Pages: 17862-17865
  • DOI: 10.1021/ja3079972
  • Peer Reviewed
• [Journal Article] Phospholipid nanodisc engineering for drug delivery systems (2012)
  • Author(s): Murakami, T.
  • Journal Title: Biotechnology Journal Volume: 7 Issue: 6 Pages: 762-767
  • DOI: 10.1002/biot.201100508
  • Peer Reviewed
• [Journal Article] Creation of pure nanodrugs and their anticancer properties (2012)
  • Author(s): H. Kasai, T. Murakami, Y. Ikuta, Y. Koseki, K. Baba, H. Oikawa, H. Nakanishi, M. Okada, M. Shoji, M. Ueda, H. Imahori, M. Hashida
  • Journal Title: Angew. Chem. Int. Ed Volume: 51 Issue: 41 Pages: 10315-10318
  • DOI: 10.1002/anie.201204596
  • Peer Reviewed
• [Journal Article] Utilization of Photo induced Charge-Separated State of Donor-Acceptor Linked Molecules for Regulation of Cell Membrane Potential and Ion Transport (2012)
  • Author(s): Numata T, Murakami T, Kawashima F, Morone N, Heuser JE, Takano Y, Ohkubo K, Fukuzumi S, Mori Y, Imahori H
  • Journal Title: J Am Chem Soc Volume: 134 Issue: 14 Pages: 6092-6095
  • DOI: 10.1021/ja3007275
  • Peer Reviewed
• [Journal Article] Single-walled carbon nanohorns as drug carriers : adsorption of prednisolone and anti-inflammatory effects on arthritis (2011)
  • Author(s): Nakamura M., Tsuchida K., 他6名
  • Journal Title: Nanotechnology Volume: 22巻 Issue: 46 Pages: 465102-465102
  • DOI: 10.1088/0957-4484/22/46/465102
  • Peer Reviewed
• [Presentation] 細胞接着性金ナノロッドの作製と細胞膜蛋白質の光制御への応用 (2014)
  • Author(s): 中辻博貴・小山祥平・木村佑・高野勇太・沼田朋大・森泰生・今堀博・村上達也
  • Organizer: 日本化学会第94回春季年会
  • Place of Presentation: 名古屋大学
• [Presentation] 細胞接着性金ナノロッドの作製と 細胞膜蛋白質の光制御への応用 (2014)
  • Author(s): 中辻博貴
  • Organizer: 日本化学会第94回春季年会
  • Place of Presentation: 名古屋大学
• [Presentation] 光応答性ナノ材料による細胞工学と難病治療 (2013)
  • Author(s): 村上達也
  • Organizer: 第10回光科学若手研究会
  • Place of Presentation: 京都大学宇治キャンパス
  • Year and Date: 2013-05-11
  • Invited
• [Presentation] ナノテクノロジーを用いた難病治療法の開発に向けて (2013)
  • Author(s): 村上達也
  • Organizer: 福井プロジェクト・グループ主催岡山大学・岡山理科大学・津山工専Tryアングル・インターネット講演会
  • Place of Presentation: 津山工専
  • Year and Date: 2013-04-05
  • Invited
• [Presentation] 生体由来ナノディスクの機能改変と生物医学応用 (2013)
  • Author(s): 村上達也
  • Organizer: 第7回ナノ・バイオメディカル学会大会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2013-01-24
  • Invited
• [Presentation] 近赤外光照射時に高い光線力学効果を発現する単層カーボンナノチューブの探索と応用 (2013)
  • Author(s): 稲田真実、村上達也、中辻博貴、梅山有和、今堀博
  • Organizer: 日本化学会第93春季年会(2013)
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
• [Presentation] 細胞膜透過性高比重リポタンパク質による金ナノロッドの生体適合化と細胞内送達 (2013)
  • Author(s): 中辻博貴、村上達也、諸根信弘、John E. Heuser、橋田充、今堀博
  • Organizer: 日本化学会第93春季年会(2013)
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
• [Presentation] Light-harvesting carbon nanomaterials for cell biology and therapy (2013)
  • Author(s): Murakami, T.
  • Organizer: RSC-iCeMS International Symposium
  • Place of Presentation: Kyoto University, Kyoto
  • Invited
• [Presentation] Enhancement of Photodynamic Effect by Single Chirality Separation of Semiconducting Single-walled Carbon Nanotubes : Application to Photodynamic Therapy (2013)
  • Author(s): Murakami, T.
  • Organizer: CBI学会2013年大会
  • Place of Presentation: タワーホール船堀
• [Presentation] カチオン性ペプチド融合高比重リポタンパク質の開発 (2013)
  • Author(s): Hyungjin Kim、岡本陽己、土田邦博、村上達也
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船堀
• [Presentation] サンドイッチ型ナフタロシアニン錯体内包リポ蛋白質の光照射による熱選択的産生と殺細胞活性 (2013)
  • Author(s): 村上達也、Simon Mathew、中辻博貴、岡本陽己、橋田充、今堀博
  • Place of Presentation: タワーホール船堀
• [Presentation] カーボンナノチューブの光線力学効果と殺細胞活性 (2013)
  • Author(s): 村上達也、中辻博貴、稲田真実、的羽良典、橋田充、今堀博
  • Organizer: 第29回日本DDS学会学術集会
  • Place of Presentation: 京都テルサ
• [Presentation] ナノテクノロジーを用いた難病治療法の開発に向けて (2013)
  • Author(s): 村上達也
  • Organizer: 福井プロジェクト・グループ主催 岡山大学・岡山理科大学・津山工専 Tryアングル・インターネット講演会
  • Place of Presentation: 津山工専
  • Invited
• [Presentation] サンドイッチ型ナフタロシアニン錯体内包リポ蛋白質の光照射による熱選択的産生と殺細胞活性 (2013)
  • Author(s): 村上達也
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船堀
• [Presentation] Enhancement of Photodynamic Effect by Single Chirality Separation of Semiconducting Single-walled Carbon Nanotubes: Application to Photodynamic Therapy (2013)
  • Author(s): 村上達也
  • Organizer: CBI学会2013年大会
  • Place of Presentation: タワーホール船堀
• [Presentation] Biochemical Engineering of Mesoscale Lipid-Protein Composite Biomaterials for Drug Delivery System (2012)
  • Author(s): Murakami, T.
  • Organizer: CLS-iCeMS Joint Symposium
  • Place of Presentation: Tsinghua University, Beijing
  • Invited
• [Presentation] 近赤外光照射時に活性酸素種を高効率生成する単層カーボンナノチューブの探索 : 光線力学的療法への応用を目指して (2012)
  • Author(s): 稲田真実、村上達也、中辻博貴、的羽良典、梅山有和、橋田充、今堀博
  • Organizer: 日本バイオマテリアル学会大会シンポジウム2012
  • Place of Presentation: 仙台国際センター
• [Presentation] ディスク状高比重リポタンパク質と金ナノロッドの融合 : 新しい有機無機複合体材料の作製と機能評価 (2012)
  • Author(s): 中辻博貴、村上達也、諸根信弘、John E. Heuser、橋田充、今堀博
  • Organizer: 日本バイオマテリアル学会大会シンポジウム2012
  • Place of Presentation: 仙台国際センター
• [Presentation] ディスク状高比重リポタンパク質と金ナノロッドの融合：新しい有機無機複合体材料の作製と機能評価 (2012)
  • Author(s): 中辻博貴、村上達也、諸根信弘、John E. Heuser、橋田充、今堀博
  • Organizer: 日本バイオマテリアル学会大会シンポジウム2012
  • Place of Presentation: 仙台国際センター
• [Presentation] 近赤外光照射時に活性酸素種を高効率生成する単層カーボンナノチューブの探索：光線力学的療法への応用を目指して (2012)
  • Author(s): 稲田真実、村上達也、中辻博貴、的羽良典、梅山有和、橋田充、今堀博
  • Organizer: 日本バイオマテリアル学会大会シンポジウム2012
  • Place of Presentation: 仙台国際センター
• [Remarks]
  • URL: http://www.murakami.icems.kyoto-u.ac.jp/
• [Remarks] 京都大学iCeMS村上グループホームページ
  • URL: http://www.murakami.icems.kyoto-u.ac.jp/